Gymnemic Acid Stimulates In Vitro Splenic Lymphocyte Proliferation

2015 ◽  
Vol 30 (2) ◽  
pp. 341-344 ◽  
Author(s):  
Vineet Kumar Singh ◽  
Padmanabh Dwivedi ◽  
B. R. Chaudhary ◽  
Ramesh Singh
Author(s):  
Ying-Wu Mei ◽  
Zhan-Qing Yang ◽  
Wei Wang ◽  
De-Guang Song ◽  
Xu-Ming Deng ◽  
...  

Background:Up to now, many “immunoactive” brain areas have been identified, such a hypothalamic nuclei, brain reward system; but the nucleus ambiguous (Amb), a nucleus nervi vagis of medulla oblongata, was less well studied in neuroimmunomodulation.Methods:In order to obtain more profound comprehension and more knowledge on Amb, we studied the effect of acute electrical stimulation of Amb on thymus and spleen activity in rat. A stimulator was applied to stimulate the Amb of the anaesthetic rats using the parameter at 100μAx5ms x100 Hz every 1s for 1 min. The levels of TGF-β and thymosin-β4 mRNA in thymus, the release of IL-2 and IL-6 at splenocyte in vitro and splenic lymphocyte proliferation were measured at hour 0.5,1,2,3 following the electrical stimulation.Results:The results showed that concanavalin A (Con A)-induced splenic lymphocyte proliferation and the release of IL-2 and IL-6 were all significantly enhanced at 0.5, 1, and 2 h following effective Amb stimulation as compared to in the control group. However, as compared to in the control group, the levels of TGF-β and thymosin-β4 mRNA in the thymus were both remarkably reduced at 0.5, 1, and 2 h following effective Amb stimulation.Conclusions:These findings reveal that the Amb participates in the modulation of animal immune functions.


1993 ◽  
Vol 66 (3) ◽  
pp. 201-211 ◽  
Author(s):  
Balu H. Athreya ◽  
Jonathan Pletcher ◽  
Francesco Zulian ◽  
David B. Weiner ◽  
William V. Williams

1981 ◽  
Vol 153 (4) ◽  
pp. 871-882 ◽  
Author(s):  
H Y Tse ◽  
J J Mond ◽  
W E Paul

For the purpose of examining more closely the interaction between T and B lymphocytes, we have developed an in vitro T lymphocyte-dependent B lymphocyte proliferation assay. Proliferation of B lymphocytes in response to antigen was found to depend on the presence of primed T lymphocytes; the B lymphocytes could be derived from nonprimed animals. It appears that these B cells were nonspecifically recruited to proliferate. This nonspecific recruitment, however, was found to be Ir-gene restricted in that B lymphocytes from B10.S mice, which are genetic nonresponders to the polymer Glu60-Ala30-Tyr10 (GAT), could not be stimulated by GAT-primed (responder X nonresponder) F1 T cells. The apparent lack of antigen specificity in the face of Ir gene-restricted T-B interaction may have important implications in our understanding of the recognition unit(s) on T lymphocytes.


2006 ◽  
Vol 164 ◽  
pp. S110-S111
Author(s):  
Maria Barca ◽  
Anne Marie Ciobanu ◽  
Dan Balalau ◽  
Daniela Luiza Baconi ◽  
Mihaela Ilie ◽  
...  

2000 ◽  
Vol 37 (6) ◽  
pp. 885-892 ◽  
Author(s):  
George B. Schoeler ◽  
Stephen A. Manweiler ◽  
Douglas K. Bergman ◽  
Stephen K. Wikel

1990 ◽  
Vol 24 (2) ◽  
pp. 61-66 ◽  
Author(s):  
Rainer Rupprecht ◽  
Norbert Wodarz ◽  
Johannes Kornhuber ◽  
Bernhard Schmitz ◽  
Klaus Wild ◽  
...  

1997 ◽  
Vol 112 (3) ◽  
pp. 212-217 ◽  
Author(s):  
Karin Cederbrant ◽  
Per Hultman ◽  
Jan A. Marcusson ◽  
Lita Tibbling

1997 ◽  
Vol 28 ◽  
pp. S114
Author(s):  
Shiki Okamoto ◽  
Hiroshi Kitamura ◽  
Kazuhiro Kimura ◽  
Masayuki Saito

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