The Amoebicidal Effect of Ergosterol Peroxide Isolated fromPleurotus ostreatus

2015 ◽  
Vol 29 (12) ◽  
pp. 1982-1986 ◽  
Author(s):  
Thuluz Meza-Menchaca ◽  
Jorge Suárez-Medellín ◽  
Christian Del Ángel-Piña ◽  
Ángel Trigos
Keyword(s):  
Ergosterol Peroxide ◽  
Amoebicidal Effect

scholarly journals Nicotinamide Effectively Suppresses Fusarium Head Blight in Wheat Plants

2021 ◽  
Vol 22 (6) ◽  
pp. 2968
Author(s):  
Yasir Sidiq ◽  
Masataka Nakano ◽  
Yumi Mori ◽  
Takashi Yaeno ◽  
Makoto Kimura ◽  
...  
Keyword(s):  
Fusarium Head Blight ◽  
Fungal Pathogen ◽  
Antifungal Compounds ◽  
Pyridine Nucleotides ◽  
Fungal Metabolites ◽  
Dna Hypomethylation ◽  
Metabolome Analysis ◽  
Head Blight ◽  
Ergosterol Peroxide ◽  
Nicotinamide Mononucleotide

Pyridine nucleotides such as a nicotinamide adenine dinucleotide (NAD) are known as plant defense activators. We previously reported that nicotinamide mononucleotide (NMN) enhanced disease resistance against fungal pathogen Fusarium graminearum in barley and Arabidopsis. In this study, we reveal that the pretreatment of nicotinamide (NIM), which does not contain nucleotides, effectively suppresses disease development of Fusarium Head Blight (FHB) in wheat plants. Correspondingly, deoxynivalenol (DON) mycotoxin accumulation was also significantly decreased by NIM pretreatment. A metabolome analysis showed that several antioxidant and antifungal compounds such as trigonelline were significantly accumulated in the NIM-pretreated spikes after inoculation of F. graminearum. In addition, some metabolites involved in the DNA hypomethylation were accumulated in the NIM-pretreated spikes. On the other hand, fungal metabolites DON and ergosterol peroxide were significantly reduced by the NIM pretreatment. Since NIM is relative stable and inexpensive compared with NMN and NAD, it may be more useful for the control of symptoms of FHB and DON accumulation in wheat and other crops.

STEROL AND FATTY ACID COMPONENTS OF MYCELIUM OF FUSARIUM OXYSPORUM

1967 ◽  
Vol 13 (10) ◽  
pp. 1351-1355 ◽  
Author(s):  
A. N. Starratt ◽  
C. Madhosingh
Keyword(s):  
Fatty Acids ◽  
Liquid Chromatography ◽  
Fatty Acid ◽  
Fusarium Oxysporum ◽  
Culture Medium ◽  
Chloroform Extract ◽  
Gas Liquid Chromatography ◽  
Ergosterol Peroxide ◽  
Oleic And Linoleic Acids

A chloroform extract of the mycelium of Fusarium oxysporum Schl. em. Sny. et Hans, was analyzed for sterols; three major components were identified as ergosterol, ergosterol peroxide, and cerevisterol. The ratio of ergosterol to ergosterol peroxide formed was variable. Palmitic, stearic, oleic, and linoleic acids were identified by gas–liquid chromatography as the principal fatty acids of this organism.Fusaric acid, a phytotoxin previously obtained by other investigators from a number of forms of F. oxysporum, was isolated from the culture medium.

Inonotus obliquus aqueous extract suppresses carbon tetrachloride induced hepatic injury through modulation of antioxidant enzyme system and anti-inflammatory mechanism

2021 ◽  
Vol 08 ◽  
Author(s):  
Pir Mohammad Ishfaq ◽  
Anjali Mishra ◽  
Shivani Mishra ◽  
Zaved Ahmad ◽  
Shovanlal Gayen ◽  
...  
Keyword(s):  
Docking Studies ◽  
Liver Inflammation ◽  
Inhibitory Mechanism ◽  
Inonotus Obliquus ◽  
Ergosterol Peroxide ◽  
Anticancer Effects ◽  
Growth Inhibitory ◽  
Inflammatory Mechanism ◽  
Cox 2 ◽  
Anti Inflammatory

Background: Chaga mushroom [Inonotus obliquus] is an edible macrofungus used in traditional and folk medicine for treatment of various gastrointestinal disorders. It has shown potent anti-inflammatory, antioxidant and anticancer effects in several experimental studies including our anti-inflammatory and anticancer effects in colorectal cancer and intestinal inflammation. Whole extract or purified compound ergosterol peroxide from chaga mushroom showed anti-inflammatory mechanism via suppression of NF-κB/iNOS-COX-2 and growth inhibitory mechanism via regulation of apoptosis activation and β-catenin suppression. The emergence of diverse inflammatory and carcinogenic agents like carbon tetrachloride [CCl4] is a potent hepatotoxic chemical that caused liver damage by inducing lipid peroxidation and other oxidative damages. Aims: The study was aimed to analyze the biochemical, cellular and molecular mechanism of CCl4 induced chronic liver inflammation and carcinoma and to analyze the effect of the extract of chaga mushroom on liver inflammation and cancer by virtue of anti-inflammatory mechanisms. Method: Physiological, histological and immunohistochemical the physiological functions and cellular functions. Biochemical assays for assessing enzymatic changes in tissues. Molecular simulation and docking studies were performed for proposing the molecular interaction. Results: CCl4-exposed mice exhibited a significant decrease in the body weight followed by altered histopathological signatures in the liver. Supplementation of IOAE showed that treatment restored towards normal structure of the tissues with large round nuclei in most of the cells. CCl4 caused a steep elevation in the levels of SGOT and SGPT to 2.32- and 1.8-fold as compared to control. The LDH level was increased to 447 IU/L in CCl4 treated mice as compared to control [236 IU/L]. Analysis of the oxidant enzyme pathway showed that CCl4 reduced the GSH level to 16.5 μM as compared to control [52 μM], and induced the catalase enzyme activity to 259 U/mL as compared to control [124 U/L]. These physiological and biochemical alterations were restored towards normal levels by IOAE administration. Immunohistochemical staining for caspase-3 and p53 showed that CCl4 notably increased their expressions which were subsequently suppressed by administration of IOAE. The molecular simulation and docking studies using ergosterol peroxide from chaga mushroom with iNOS, COX-2 and TNF-α showed binding energy of -10.5, -8.9 and -9.1 Kcal/mol, respectively. These proteins interacting with ergosterol peroxide suggests an inhibitory effect on these critical proinflammatory signaling proteins. Conclusions: The results point out that IOAE is able to prevent damage of hepatic cells caused by CCl4 in mouse models through anti-inflammatory and growth inhibitory mechanism which can be utilized in natural prevention of the liver toxicity.

8-Isocyanoamphilecta-11(20),15-diene, a new antimalarial isonitrile diterpene from the sponge Ciocalapata sp.

2009 ◽  
Vol 87 (5) ◽  
pp. 612-618 ◽  
Author(s):  
Chatchai Wattanapiromsakul ◽  
Naphatson Chanthathamrongsiri ◽  
Somchai Bussarawit ◽  
Supreeya Yuenyongsawad ◽  
Anuchit Plubrukarn ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Cell Lines ◽  
Nmr Spectra ◽  
Human Fibroblasts ◽  
Fibroblast Cell ◽  
The Other ◽  
Ergosterol Peroxide ◽  
Other Hand ◽  
Mcf 7 ◽  
Fibroblast Cell Lines

A new isonitrile diterpene of the amphilectane family, 8-isocyanoamphilecta-11(20),15-diene (4), was isolated from the sponge Ciocalapata sp., along with three known isonitriles, 8,15-diisocyano-11(20)-amphilectene (1), 7-isocyanoamphilecta-11(20),15-diene (2), and 8-isocyanoamphilecta-11(20),14-diene (3), and two steroidal peroxides, ergosterol peroxide (5) and 5α,9α-epidioxy-8α,14α-epoxy-(22E)-ergosta-6,22-dien-3β-ol (6). The structure of the new isonitrile was elucidated spectroscopically. In addition, anomalous multiplicities in the NMR spectra of some isolated isonitriles were observed and are reported here. The four isonitriles were strongly active against Plasmodium falciparum K1 with IC50 in a range of 0.09–1.07 μmol/L. Except for 1, which was cytotoxic against both MCF-7 and fibroblast cell lines, the other three diterpenes showed no significant cytotoxicity against either targeted cell lines. On the other hand, the steroidal peroxides 5 and 6, which were less active in the antimalarial bioassay (IC50 values of 6.28 and 7.13 µmol/L, respectively), were strongly cytotoxic against MCF-7 (IC50 values of 0.025 and 0.003 µmol/L, respectively), with very little toxicity against human fibroblasts.

scholarly journals Transformation of Ergosterol Peroxide to Cytotoxic Substances by Rat Intestinal Bacteria

2008 ◽  
Vol 31 (5) ◽  
pp. 949-954 ◽  
Author(s):  
Joo-Sang Lee ◽  
Chao-Mei Ma ◽  
Dong-Ki Park ◽  
Yasuharu Yoshimi ◽  
Minoru Hatanaka ◽  
...  
Keyword(s):  
Intestinal Bacteria ◽  
Ergosterol Peroxide

Abstract 1869: Mechanistic evaluation of ergosterol peroxide onin vitrobreast cancer models

2019 ◽  
Author(s):  
Camille L. Santiago ◽  
Fatima Rivas ◽  
Taotao Ling ◽  
Ivette J. Suarez-Arroyo ◽  
Gabriela Ortiz ◽  
...  
Keyword(s):  
Ergosterol Peroxide ◽  
Cancer Models

scholarly journals Ergosterol Peroxide from the Medicinal Mushroom Ganoderma lucidum Inhibits Differentiation and Lipid Accumulation of 3T3-L1 Adipocytes

2020 ◽  
Vol 21 (2) ◽  
pp. 460 ◽  
Author(s):  
Yong-Un Jeong ◽  
Young-Jin Park
Keyword(s):  
Transcription Factors ◽  
Fatty Acid ◽  
Fatty Acid Synthase ◽  
Metabolic Diseases ◽  
Binding Protein ◽  
Regulatory Element ◽  
Peroxisome Proliferator ◽  
Mrna Levels ◽  
Medicinal Mushrooms ◽  
Ergosterol Peroxide

Ergosterol peroxide is a natural compound of the steroid family found in many fungi, and it possesses antioxidant, anti-inflammatory, anticancer and antiviral activities. The anti-obesity activity of several edible and medicinal mushrooms has been reported, but the effect of mushroom-derived ergosterol peroxide on obesity has not been studied. Therefore, we analyzed the effect of ergosterol peroxide on the inhibition of triglyceride synthesis at protein and mRNA levels and differentiation of 3T3-L1 adipocytes. Ergosterol peroxide inhibited lipid droplet synthesis of differentiated 3T3-L1 cells, expression of peroxisome proliferator-activated receptor gamma (PPARγ) and CCAT/enhancer-binding protein alpha (C/EBPα), the major transcription factors of differentiation, and also the expression of sterol regulatory element-binding protein-1c (SREBP-1c), which promotes the activity of PPARγ, resulting in inhibition of differentiation. It further inhibited the expression of fatty acid synthase (FAS), fatty acid translocase (FAT), and acetyl-coenzyme A carboxylase (ACC), which are lipogenic factors. In addition, it inhibited the phosphorylation of mitogen-activated protein kinases (MAPKs) involved in cell proliferation and activation of early differentiation transcription factors in the mitotic clonal expansion (MCE) stage. As a result, ergosterol peroxide significantly inhibited the synthesis of triglycerides and differentiation of 3T3-L1 cells, and is, therefore, a possibile prophylactic and therapeutic agent for obesity and related metabolic diseases.

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