scholarly journals Some South African Rubiaceae Tree Leaf Extracts Have Antimycobacterial Activity Against Pathogenic and Non-pathogenic Mycobacterium Species

2015 ◽  
Vol 29 (7) ◽  
pp. 1004-1010 ◽  
Author(s):  
Abimbola O. Aro ◽  
Jean P. Dzoyem ◽  
Tiny M. Hlokwe ◽  
Evelyn Madoroba ◽  
Jacobus N. Eloff ◽  
...  
2018 ◽  
Vol 12 (05) ◽  
pp. 726-730 ◽  
Author(s):  
Matheus Ferreira França Teixeira ◽  
◽  
Daniel Teixeira Pinheiro ◽  
Hamilton Carvalho Santos Junior ◽  
Ediane Conceição Alves ◽  
...  

2010 ◽  
Vol 29 (12) ◽  
pp. 1529-1533 ◽  
Author(s):  
S.M. Mahlo ◽  
L.J. McGaw ◽  
J.N. Eloff

2020 ◽  
Author(s):  
◽  
David Serunjogi ◽  
Kizito Muwonge

Background: Plant species such as the guava have been used in Uganda and elsewhere in the world to treat some of the medical conditions associated with bacteria, this is due to the increased number of drug resistant bacteria in the world. In this study, the antimicrobial activity of guava leaf extracts against some of the disease causing bacteria isolated from Kisubi hospital in Uganda is discussed. Methods: The guava leafs were collected from a plantation near Zika forest in Uganda (0°7′27″N 32°31′32″E / 0.12417°N 32.52556°E / 0.12417; 32.52556) and samples were put in a bag and transferred to the university laboratory where they were identified. The extracts were obtained by maceration using distilled water, 30%, 50% and 70 % methanol as the extraction solvents. Antimicrobial susceptibility testing was conducted using the disc diffusion method. Results: Gram-negative Escherichia coli was sensitive to the plant extract and synthetic commercial drugs such as trimethoprim-sulfamethozole, ciprofloxacin, and Gentamicin. Pseudomonas aeruginosa was resistant to all drugs. Streptococcus pneumonia and Staphylococcus aureus were all sensitive to the plant extracts with measurable inhibition zones. Conclusion: The Guava tree leaf crude extracts have antimicrobial activity against drug-resistant bacteria. More studies should be carried out to know the potency and the concentration of different plant origin extracts.


Author(s):  
P. Nivethadevi ◽  
C. Swaminathan ◽  
P. Kannan ◽  
E. Tamilselvi

Background: Blackgram (Vigna mungo L.), the most important highly prized pulse crops is grown throughout India. The productivity can be evaluated through various input management practices including inorganic and organic as well as natural way of cultivation. Methods: Both lab and field investigations were carried out to choose best tree leaf extracts for seed fortification and foliar nutrition in blackgram and also to evaluate performance of Vrikshayurvedic farming practices with seed fortification and foliar spraying of Moringa oleifera leaf extracts and basal nutrition of Delonix regia. Comparison with conventional scientific practices and do-nothing farming practice was also made. Result: Vrikshayurvedic farming (Delonix regia + Moringa oleifera @ 5%) as green leaf manure and foliar spray with tree leaf extract, respectively, registered highest vales for growth and yield parameters. It was at par with conventional scientific practice (RDF @ 25:50:25 NPK kg ha-1 and foliar spray with DAP 2% twice @ 30 and 45 DAS). It is inferred that vrikshayurveda treatments resulted in better plant growth, DMP, seed yield, pod yield equivalent as that of conventional practice and superior to do-nothing practice. It also helps to improve and maintain soil fertility over a long period of time.


2012 ◽  
Vol 2012 ◽  
pp. 1-9 ◽  
Author(s):  
Antoinette Labuschagné ◽  
Ahmed A. Hussein ◽  
Benjamín Rodríguez ◽  
Namrita Lall

Euclea natalensisA.DC.,Knowltonia vesicatoria(L.f) Sims, andPelargonium sidoidesDC. are South African plants traditionally used to treat tuberculosis. Extracts from these plants were used in combination with isoniazid (INH) to investigate the possibility of synergy with respect to antimycobacterial activity. The ethanol extract ofK. vesicatoriawas subjected to fractionation to identify the active compounds. The activity of theKnowltoniaextract remained superior to the fractions with a minimum inhibitory concentration (MIC) of 625.0 μg/mL againstMycobacterium smegmatisand an MIC of 50.00 μg/mL againstM. tuberculosis. TheK. vesicatoriaextract was tested against two different drug-resistant strains ofM. tuberculosis, which resulted in an MIC of 50.00 μg/mL on both strains. The combination ofK. vesicatoriawith INH exhibited the best synergistic antimycobacterial activity with a fractional inhibitory concentration index of 0.25 (a combined concentration of 6.28 μg/mL). A fifty percent inhibitory concentration of this combination against U937 cells was 121.0 μg/mL. Two compounds, stigmasta-5,23-dien-3-ol (1) and 5-(hydroxymethyl)furan-2(5H)-one (2), were isolated fromK. vesicatoriaas the first report of isolation for both compounds from this plant and the first report of antimycobacterial activity. Compound (1) was active against drug-sensitiveM. tuberculosiswith an MIC of 50.00 μg/mL.


2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Rocio Gómez-Cansino ◽  
Clara Inés Espitia-Pinzón ◽  
María Guadalupe Campos-Lara ◽  
Silvia Laura Guzmán-Gutiérrez ◽  
Erika Segura-Salinas ◽  
...  

The extracts of 14 Julianaceae and 5 Clusiaceae species growing in Mexico were testedin vitro(50 µg/mL) againstMycobacterium tuberculosisH37Rv and HIV reverse transcriptase (HIV-RT). The Julianaceae bark and leaf extracts inhibitedM. tuberculosis(>84.67%) and HIV-RT (<49.89%). The Clusiaceae leaves extracts also inhibited both targets (>58.3% and >67.6%), respectively. The IC50values for six selected extracts and their cytotoxicity (50 µg/mL) to human macrophages were then determined.Amphipterygium glaucum,A. molle, andA. simplicifoliumfairly inhibitedM. tuberculosiswith IC50of 1.87–2.35 µg/mL; but their IC50against HIV-RT was 59.25–97.83 µg/mL.Calophyllum brasiliense,Vismia baccifera, andVismia mexicanaeffect onM. tuberculosiswas noteworthy (IC503.02–3.64 µg/mL) and also inhibited RT-HIV (IC5026.24–35.17 µg/mL). These 6 extracts (50 µg/mL) presented low toxicity to macrophages (<23.8%). The HPLC profiles ofA. glaucum,A. molle, andA. simplicifoliumindicated that their antimycobacterial activity cannot be related to masticadienonic, 3α, or 3β-hydromasticadienonic acids, suggesting that other compounds may be responsible for the observed activity or this might be a synergy result. The anti-HIV-RT and antimycobacterial activities induced byC. brasiliensecan be attributed to the content of calanolides A, B, as well as soulatrolide.


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