Ferulate Protects the Epithelial Barrier by Maintaining Tight Junction Protein Expression and Preventing Apoptosis in Tert -Butyl Hydroperoxide-Induced Caco-2 Cells

2012 ◽  
Vol 27 (3) ◽  
pp. 362-367 ◽  
Author(s):  
Hyun Jung Kim ◽  
Eun Kyeong Lee ◽  
Min Hi Park ◽  
Young Mi Ha ◽  
Kyung Jin Jung ◽  
...  
Keyword(s):  
Protein Expression ◽  
Tight Junction ◽  
Tight Junction Protein ◽  
Epithelial Barrier ◽  
Butyl Hydroperoxide ◽  
Tert Butyl ◽  
Tight Junction Protein Expression ◽  
Tert Butyl Hydroperoxide ◽  
Junction Protein

Probiotic mixture VSL#3 protects the epithelial barrier by maintaining tight junction protein expression and preventing apoptosis in a murine model of colitis

2009 ◽  
Vol 296 (5) ◽  
pp. G1140-G1149 ◽  
Author(s):  
Rudolf Mennigen ◽  
Kerstin Nolte ◽  
Emile Rijcken ◽  
Markus Utech ◽  
Bettina Loeffler ◽  
...  
Keyword(s):  
Protein Expression ◽  
Tight Junction ◽  
Tight Junction Protein ◽  
Epithelial Barrier ◽  
Epithelial Permeability ◽  
Acute Colitis ◽  
Pronounced Increase ◽  
Tight Junction Protein Expression ◽  
Junction Protein ◽  
Healthy Control

Changes in epithelial tight junction protein expression and apoptosis increase epithelial permeability in inflammatory bowel diseases. The effect of the probiotic mixture VSL#3 on the epithelial barrier was studied in dextran sodium sulfate (DSS)-induced colitis in mice. Acute colitis was induced in BALB/c mice (3.5% DSS for 7 days). Mice were treated with either 15 mg VSL#3 or placebo via gastric tube once daily during induction of colitis. Inflammation was assessed by clinical and histological scores. Colonic permeability to Evans blue was measured in vivo. Tight junction protein expression and epithelial apoptotic ratio were studied by immunofluorescence and Western blot. VSL#3 treatment reduced inflammation (histological colitis scores: healthy control 0.94 ± 0.28, DSS + placebo 14.64 ± 2.55, DSS + VSL#3 8.43 ± 1.82; P = 0.011). A pronounced increase in epithelial permeability in acute colitis was completely prevented by VSL#3 therapy [healthy control 0.4 ± 0.07 (extinction/g), DSS + placebo 5.75 ± 1.67, DSS + VSL#3 0.26 ± 0.08; P = 0.003]. In acute colitis, decreased expression and redistribution of the tight junction proteins occludin, zonula occludens-1, and claudin-1, -3, -4, and -5 were observed, whereas VSL#3 therapy prevented these changes. VSL#3 completely prevented the increase of epithelial apoptotic ratio in acute colitis [healthy control 1.58 ± 0.01 (apoptotic cells/1,000 epithelial cells), DSS + placebo 13.33 ± 1.29, DSS + VSL#3 1.72 ± 0.1; P = 0.012]. Probiotic therapy protects the epithelial barrier in acute colitis by preventing 1) decreased tight junction protein expression and 2) increased apoptotic ratio.

Changes in tight junction protein expression in intrinsic aging and photoaging in human skin in vivo

2016 ◽  
Vol 84 (1) ◽  
pp. 99-101 ◽  
Author(s):  
Seon-Pil Jin ◽  
Sang Bum Han ◽  
Yeon Kyung Kim ◽  
Elizabeth Eunkyung Park ◽  
Eun Jin Doh ◽  
...  
Keyword(s):  
Protein Expression ◽  
Tight Junction ◽  
Human Skin ◽  
Tight Junction Protein ◽  
Tight Junction Protein Expression ◽  
Junction Protein

Granny Smith apple procyanidin extract upregulates tight junction protein expression and modulates oxidative stress and inflammation in lipopolysaccharide-induced Caco-2 cells

2018 ◽  
Vol 9 (6) ◽  
pp. 3321-3329 ◽  
Author(s):  
H. Wu ◽  
T. Luo ◽  
Y. M. Li ◽  
Z. P. Gao ◽  
K. Q. Zhang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Protein Expression ◽  
Tight Junction ◽  
Tight Junction Protein ◽  
Tight Junction Protein Expression ◽  
Junction Protein

Granny Smith apple procyanidin extracts upregulate tight junction protein expression, probably acting via the modulation of oxidative stress and inflammation in lipopolysaccharide-induced Caco-2 cells.

Effects of di(2-ethylhexyl) phthalate on gap and tight junction protein expression in the testis of prepubertal rats

2009 ◽  
Vol 72 (11) ◽  
pp. 868-877 ◽  
Author(s):  
Cristian M. Sobarzo ◽  
Livia Lustig ◽  
Roberto Ponzio ◽  
María Olga Suescun ◽  
Berta Denduchis
Keyword(s):  
Protein Expression ◽  
Tight Junction ◽  
Tight Junction Protein ◽  
Tight Junction Protein Expression ◽  
Junction Protein ◽  
Prepubertal Rats ◽  
Ethylhexyl Phthalate
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