Effect of berberine and Berberis aetnensis C. Presl. alkaloid extract on glutamate-evoked tissue transglutaminase up-regulation in astroglial cell cultures

2010 ◽  
Vol 25 (6) ◽  
pp. 816-820 ◽  
Author(s):  
Agata Campisi ◽  
Rosaria Acquaviva ◽  
Silvana Mastrojeni ◽  
Giuseppina Raciti ◽  
Angelo Vanella ◽  
...  
Keyword(s):  
Cell Cultures ◽  
Tissue Transglutaminase ◽  
Astroglial Cell ◽  
Astroglial Cell Cultures

scholarly journals Antioxidant Properties ofBerberis aetnensisC. Presl (Berberidaceae) Roots Extract and Protective Effects on Astroglial Cell Cultures

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Agata Campisi ◽  
Rosaria Acquaviva ◽  
Roberta Bonfanti ◽  
Giuseppina Raciti ◽  
Andrea Amodeo ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Cell Cultures ◽  
Superoxide Anion ◽  
Tissue Transglutaminase ◽  
Antioxidant Properties ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Astroglial Cell ◽  
Free System ◽  
Astroglial Cell Cultures

Berberis aetnensisC. Presl (Berberidaceae) is a bushy-spiny shrub common on Mount Etna (Sicily). We demonstrated that the alkaloid extract of roots ofB. aetnensisC. Presl contains prevalently berberine and berbamine, possesses antimicrobial properties, and was able to counteract the upregulation evoked by glutamate of tissue transglutaminase in primary rat astroglial cell cultures. Until now, there are no reports regarding antioxidant properties ofB. aetnensisC. Presl collected in Sicily. Air-dried, powdered roots ofB. aetnensisC. Presl were extracted, identified, and quantified by HPLC. We assessed in cellular free system its effect on superoxide anion, radicals scavenging activity of antioxidants against free radicals like the 1,1-diphenyl-2-picrylhydrazyl radical, and the inhibition of xanthine oxidase activity. In primary rat astroglial cell cultures, exposed to glutamate, we evaluated the effect of the extract on glutathione levels and on intracellular production of reactive oxygen species generated by glutamate. The alkaloid extract ofB. aetnensisC. Presl inhibited superoxide anion, restored to control values, the decrease of GSH levels, and the production of reactive oxygen species. Potent antioxidant activities of the alkaloid extract of roots ofB. aetnensisC. Presl may be one of the mechanisms by which the extract is effective against health disorders associated to oxidative stress.

JAK/STAT signaling pathway mediates cytokine-induced iNOS expression in primary astroglial cell cultures

2001 ◽  
Vol 65 (5) ◽  
pp. 417-424 ◽  
Author(s):  
P. Dell'Albani ◽  
R. Santangelo ◽  
L. Torrisi ◽  
V.G. Nicoletti ◽  
J. de Vellis ◽  
...  
Keyword(s):  
Signaling Pathway ◽  
Cell Cultures ◽  
Inos Expression ◽  
Astroglial Cell ◽  
Stat Signaling ◽  
Astroglial Cell Cultures

Thyroid hormone action: induction of morphological changes and protein secretion in astroglial cell cultures

1991 ◽  
Vol 58 (1) ◽  
pp. 43-49 ◽  
Author(s):  
Jean-Michel Gavaret ◽  
Daniele Toru-Delbauffe ◽  
Denise Baghdassarian-Chalaye ◽  
Martine Pomerance ◽  
Michel Pierre
Keyword(s):  
Thyroid Hormone ◽  
Cell Cultures ◽  
Protein Secretion ◽  
Morphological Changes ◽  
Hormone Action ◽  
Astroglial Cell ◽  
Thyroid Hormone Action ◽  
Astroglial Cell Cultures

Alpha-Lipoic Acid Modulates GFAP, Vimentin, Nestin, Cyclin D1 and MAP-Kinase Espression in Astroglial Cell Cultures

2010 ◽  
Vol 35 (12) ◽  
pp. 2070-2077 ◽  
Author(s):  
V. Bramanti ◽  
D. Tomassoni ◽  
D. Bronzi ◽  
S. Grasso ◽  
M. Currò ◽  
...  
Keyword(s):  
Map Kinase ◽  
Cyclin D1 ◽  
Cell Cultures ◽  
Lipoic Acid ◽  
Alpha Lipoic Acid ◽  
Astroglial Cell ◽  
Astroglial Cell Cultures
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