Antimicrobial activity and secondary structure of a novel peptide derived from ovalbumin

2020 ◽  
Vol 26 (10) ◽  
Author(s):  
Ao Tan ◽  
Rio Suzuki ◽  
Chikako Yokoyama ◽  
Shigekazu Yano ◽  
Hiroyuki Konno
Keyword(s):  
Antimicrobial Activity ◽  
Secondary Structure

Dermaseptins from Phyllomedusa oreades and Phyllomedusa distincta: Secondary structure, antimicrobial activity, and mammalian cell toxicity

2008 ◽  
Vol 151 (3) ◽  
pp. 336-343 ◽  
Author(s):  
José Roberto S.A. Leite ◽  
Guilherme D. Brand ◽  
Luciano P. Silva ◽  
Selma A.S. Kückelhaus ◽  
Wilian R.C. Bento ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Secondary Structure ◽  
Mammalian Cell ◽  
Cell Toxicity ◽  
Mammalian Cell Toxicity

scholarly journals Effect of intramolecular disulfide bond of bovine lactoferricin on its molecular structure and antibacterial activity against Trueperella pyogenes separated from cow milk with mastitis

2020 ◽  
Vol 16 (1) ◽  
Author(s):  
Jie Pei ◽  
Lin Xiong ◽  
Min Chu ◽  
Xian Guo ◽  
Ping Yan
Keyword(s):  
Molecular Structure ◽  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Secondary Structure ◽  
Disulfide Bond ◽  
Random Coil ◽  
Cow Milk ◽  
E Coli ◽  
Trueperella Pyogenes ◽  
Intramolecular Disulfide Bond

Abstract Background Lactoferricin (Lfcin) is an antimicrobial activity center of lactoferrin, produced by hydrolysis from the N-terminal of lactoferrin. It was hypothesized that the intramolecular disulfide bond in Lfcin could affect its antibacterial function through influencing its molecular structure. To prove this hypothesis, bovine Lfcin (bLfcin) and its two derivatives, bLfcin with an intramolecular disulfate bond (bLfcin DB) and bLfcin with a mutation C36G (bLfcin C36G), were synthesized, purified, and identified. The circular dichroism spectra of the peptides were detected in solutions with different ionic and hydrophobic strength. The antibacterial activity of the peptides against Trueperella pyogenes, separated from cow milk with mastitis, were determined. Results The secondary structure of bLfcin DB showed more β-turn and less random coil than the other peptides in H2O, similar ratios of secondary structures with bLfcin and bLfcin C36G under ionic conditions, and close percentages of secondary structure with bLfcin under hydrophobic conditions. The synthetic peptides exhibited strong antimicrobial activity against T. pyogenes isolates, T. pyogenes ATCC 19,411, and E. coli ATCC 25,922. The antimicrobial activities of the three peptides were greater against T. pyogenes than against E. coli, and bLfcin DB exhibited higher antibacterial activity compared with its derivatives. Conclusions The intramolecular disulfide bond could change the molecular structure of bLfcin under alternative ionic strengths and hydrophobic effects, and the formation of the disulfide bond is beneficial to executing the antibacterial function of bLfcin.

Effects and mechanisms of the secondary structure on the antimicrobial activity and specificity of antimicrobial peptides

2015 ◽  
Vol 21 (7) ◽  
pp. 561-568 ◽  
Author(s):  
Xuan-thanh Mai ◽  
Jinfeng Huang ◽  
Juanjuan Tan ◽  
Yibing Huang ◽  
Yuxin Chen
Keyword(s):  
Antimicrobial Activity ◽  
Secondary Structure ◽  
Antimicrobial Peptides

scholarly journals MUC7 20-Mer: Investigation of Antimicrobial Activity, Secondary Structure, and Possible Mechanism of Antifungal Action

2003 ◽  
Vol 47 (2) ◽  
pp. 643-652 ◽  
Author(s):  
Libuse A. Bobek ◽  
Hongsa Situ
Keyword(s):  
Antimicrobial Activity ◽  
Secondary Structure ◽  
Structure Prediction ◽  
Secondary Structure Prediction ◽  
Divalent Cations ◽  
Fungal Cell ◽  
Histatin 5 ◽  
Salivary Mucin ◽  
Antifungal Action ◽  
Cellular Metabolic Activity

ABSTRACT This study was aimed at examining the spectrum of antimicrobial activity of MUC7 20-mer (N-LAHQKPFIRKSYKCLHKRCR-C; residues 32 to 51 of MUC7, the low-molecular-weight human salivary mucin, comprised of 357 residues) and comparing its antifungal properties to those of salivary histatin 5 (Hsn-5). We also examined the secondary structure of the 20-mer and the possible mechanism of its antifungal action. Our results showed that MUC7 20-mer displays potent killing activity against a variety of fungi and both gram-positive and gram-negative bacteria at micromolar concentrations. Time-dependent killing of Candida albicans and Cryptococcus neoformans by MUC7 20-mer and Hsn-5 indicated differences in killing rates between MUC7 20-mer and Hsn-5. The secondary structure prediction showed that MUC7 20-mer adopts an amphiphilic helix with distinguishable hydrophilic and hydrophobic faces (a characteristic that is associated with antimicrobial activity). In comparison to that of Hsn-5, the fungicidal activity of MUC7 20-mer against C. albicans seems to be independent of fungal cellular metabolic activity, as evidenced by its killing potency at a low temperature (4°C) and in the presence of inhibitors of oxidative phosphorylation in the mitochondrial system. Fluorescence microscopy showed the ability of MUC7 20-mer to cross the fungal cell membrane and to accumulate inside the cells. The internalization of MUC7 20-mer was inhibited by divalent cations. Confocal microscopy of cells doubly labeled with MUC7 20-mer and a mitochondrion-specific dye indicated that mitochondria are not the target of MUC7 20-mer for either C. albicans or C. neoformans.

scholarly journals Isolation and Characterization of an Endophytic Fungus Colletotrichum coccodes Producing Tyrosol From Houttuynia cordata Thunb. Using ITS2 RNA Secondary Structure and Molecular Docking Study

2021 ◽  
Vol 9 ◽  
Author(s):  
Rajreepa Talukdar ◽  
Srichandan Padhi ◽  
Amit K. Rai ◽  
Marco Masi ◽  
Antonio Evidente ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Molecular Docking ◽  
Secondary Structure ◽  
Endophytic Fungus ◽  
Rna Secondary Structure ◽  
Plant Pathogens ◽  
Colletotrichum Coccodes ◽  
Fungal Culture ◽  
Houttuynia Cordata ◽  
Isolation And Characterization

An endophytic fungus isolated from healthy leaf tissues of Houttuynia cordata Thunb., an ethnomedicinal plant of North East India, showed a considerable amount of antimicrobial activity. The ethyl acetate extract of the fungal culture filtrates displayed promising antimicrobial activity against a panel of clinically significant pathogens including Candida albicans, Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa. Bioassay guided purification of the organic extract using column and thin layer chromatography yielded a pure homogenous compound which was identified using spectroscopic methods (essentially by 1H NMR and MS) as tyrosol, a well-known phenylethanoid present in several natural sources. Besides, molecular docking studies against tyrosyl tRNA synthetases (TyrRS) of S. aureus (PDB ID: 1JIL) and E. coli (PDB ID: 1VBM), and CYP45014α-lanosterol demethylase (CYP51) of C. albicans (PDB ID: 5FSA) revealed tyrosol has a strong binding affinity with the enzyme active site residues. The fungus was identified as Colletotrichum sp. and characterized by its genomic ITS rDNA and ITS2 sequences. Phylogenetic analyses showed clustering of our isolate with Colletotrichum coccodes. Species of Colletotrichum are also reported to be plant pathogens. Therefore, to confirm the endophytic lifestyle of the isolate, ITS2 RNA secondary structure study was undertaken. The result indicated our isolate exhibited differences in the folding pattern as well as in motif structures when compared to those of pathogenic C. coccodes. The findings indicated that endophytic fungi harboring H. cordata could be explored as a potent source of antimicrobial agents.

scholarly journals An acidic model pro-peptide affects the secondary structure, membrane interactions and antimicrobial activity of a crotalicidin fragment

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Nelson G. O. Júnior ◽  
Marlon H. Cardoso ◽  
Elizabete S. Cândido ◽  
Daniëlle van den Broek ◽  
Niek de Lange ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Secondary Structure ◽  
Membrane Interactions

Role and modulation of the secondary structure of antimicrobial peptides to improve selectivity

2020 ◽  
Vol 8 (24) ◽  
pp. 6858-6866
Author(s):  
Yangbin Liang ◽  
Xinshuang Zhang ◽  
Yueling Yuan ◽  
Yan Bao ◽  
Menghua Xiong
Keyword(s):  
Antimicrobial Activity ◽  
Secondary Structure ◽  
Antimicrobial Peptides

Helix is a two-edged sword for AMPs, and conformational modulation of AMPs can control the balance between antimicrobial activity and toxicity.

Effect of high pressure on the antimicrobial activity and secondary structure of the bacteriocin nisin

2018 ◽  
Vol 47 ◽  
pp. 9-15 ◽  
Author(s):  
Chloé Modugno ◽  
Camille Loupiac ◽  
Antoine Bernard ◽  
Audrey Jossier ◽  
Fabrice Neiers ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
High Pressure ◽  
Secondary Structure

A Comparative Study of the Antimicrobial and Structural Properties of Short Peptides and Lipopeptides Containing a Repetitive Motif KLFK

2019 ◽  
Vol 26 (3) ◽  
pp. 192-203
Author(s):  
María Verónica Húmpola ◽  
María Carolina Rey ◽  
Pablo Gabriel Spontón ◽  
Arturo Carlos Simonetta ◽  
Georgina Guadalupe Tonarelli
Keyword(s):  
Antimicrobial Activity ◽  
Secondary Structure ◽  
Mammalian Cells ◽  
Therapeutic Index ◽  
Dilution Method ◽  
Hemolysis Assay ◽  
Improved Stability ◽  
Repetitive Motif ◽  
Bacteria And Fungi ◽  
Α Helix

Background:In the last years, Antimicrobial Peptides (AMPs) and lipopeptides have received attention as promising candidates to treat infections caused by resistant microorganisms. </P><P> Objective: The main objective of this study was to investigate the effect of repetitive KLFK motifs and the attachment of aliphatic acids to the N-terminus of (KLFK)n peptides on therapeutic properties.Methods:Minimal inhibitory concentration against Gram (+) and (-) bacteria and yeast of synthetic compounds were determined by broth microtiter dilution method, and the toxicity was evaluated by hemolysis assay. Membrane-peptide interaction studies were performed with model phospholipid membranes mimicking those of bacterial and mammalian cells by Fluorescence Spectroscopy. The secondary structure in solution and membranes was determined by Circular Dichroism.Results:Our results showed that the resulting compounds have inhibitory activity against bacteria and fungi. The (KLFK)3 peptide showed the highest therapeutic index against bacterial and yeast strains, and the (KLFK)2 peptide conjugated with octanoic acid was the most active against yeasts. All the lipopeptides containing long-chain fatty acids (C14 or longer) were highly hemolytic at low concentrations. The antimicrobial activity of (KLFK)2 and (KLFK)3 lipopeptides was mainly associated with improved stability of the amphipathic secondary structure, which showed high contributions of α-helix in dipalmitoylphosphatidylglycerol (DPPG) vesicles.Conclusion:The repetition of the KLFK sequence and the conjugation with lipid tails allowed obtained compounds with high antimicrobial activity and low toxicity, becoming good candidates for treating infectious diseases.

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