scholarly journals Structural characterization of triple transmembrane domain containing fragments of a yeast G protein-coupled receptor in an organic : aqueous environment by solution-state NMR spectroscopy

2015 ◽  
Vol 21 (3) ◽  
pp. 212-222 ◽  
Author(s):  
Katrina E. Fracchiolla ◽  
Leah S. Cohen ◽  
Boris Arshava ◽  
Martin Poms ◽  
Oliver Zerbe ◽  
...  
Keyword(s):  
Nmr Spectroscopy ◽  
G Protein ◽  
Structural Characterization ◽  
Transmembrane Domain ◽  
Aqueous Environment ◽  
G Protein Coupled Receptor ◽  
Solution State ◽  
G Protein Coupled

scholarly journals Characterization of Two Fly LGR (Leucine-Rich Repeat-Containing, G Protein-Coupled Receptor) Proteins Homologous to Vertebrate Glycoprotein Hormone Receptors: Constitutive Activation of Wild-Type Fly LGR1 But Not LGR2 in Transfected Mammalian Cells**This study was supported by NIH Grant HD-23273. The GenBank submission number for fly LGR2 is AF274591.

Endocrinology ◽  
2000 ◽  
Vol 141 (11) ◽  
pp. 4081-4090 ◽  
Author(s):  
Shinya Nishi ◽  
Sheau Yu Hsu ◽  
Karen Zell ◽  
Aaron J. W. Hsueh
Keyword(s):  
G Protein ◽  
Hormone Receptors ◽  
Coupling Mechanism ◽  
Leucine Rich Repeat ◽  
G Protein Coupled Receptor ◽  
Glycoprotein Hormone ◽  
Glycoprotein Hormone Receptors ◽  
Signaling Mechanisms ◽  
G Protein Coupled

Abstract The receptors for lutropin (LH), FSH, and TSH belong to the large G protein-coupled receptor (GPCR) superfamily and are unique in having a large N-terminal extracellular (ecto-) domain important for interactions with the large glycoprotein hormone ligands. Recent studies indicated the evolution of a large family of the leucine-rich repeat-containing, G protein-coupled receptors (LGRs) with at least seven members in mammals. Based on the sequences of mammalian glycoprotein hormone receptors, we have identified a new LGR in Drosophila melanogaster and named it as fly LGR2 to distinguish it from the previously reported fly LH/FSH/TSH receptor (renamed as fly LGR1). Genomic analysis indicated the presence of 10 exons in fly LGR2 as compared with 16 exons in fly LGR1. The deduced fly LGR2 complementary DNA (cDNA) showed 43 and 64% similarity to the fly LGR1 in the ectodomain and transmembrane region, respectively. Comparison of 12 LGRs from diverse species indicated that these proteins can be divided into three subfamilies and fly LGR1 and LGR2 belong to different subfamilies. Potential signaling mechanisms were tested in human 293T cells overexpressing the fly receptors. Of interest, fly LGR1, but not LGR2, showed constitutive activity as reflected by elevated basal cAMP production in transfected cells. The basal activity of fly LGR1 was further augmented following point mutations of key residues in the intracellular loop 3 or transmembrane VI, similar to those found in patients with familial male precocious puberty. The present study reports the cloning of fly LGR2 and indicates that the G protein-coupling mechanism is conserved in fly LGR1 as compared with the mammalian glycoprotein hormone receptors. The characterization of fly receptors with features similar to mammalian glycoprotein hormone receptors allows a better understanding of the evolution of this unique group of GPCRs and future elucidation of their ligand signaling mechanisms.

scholarly journals Identification and characterization of a G protein-coupled receptor homolog encoded by murine cytomegalovirus.

1997 ◽  
Vol 71 (2) ◽  
pp. 1521-1529 ◽  
Author(s):  
N J Davis-Poynter ◽  
D M Lynch ◽  
H Vally ◽  
G R Shellam ◽  
W D Rawlinson ◽  
...  
Keyword(s):  
G Protein ◽  
Murine Cytomegalovirus ◽  
G Protein Coupled Receptor ◽  
G Protein Coupled ◽  
Identification And Characterization

Characterization of a G Protein-Coupled Receptor for Nicotinic Acid

2001 ◽  
Vol 59 (2) ◽  
pp. 349-357 ◽  
Author(s):  
Anna Lorenzen ◽  
Christina Stannek ◽  
Heidrun Lang ◽  
Viktor Andrianov ◽  
Ivars Kalvinsh ◽  
...  
Keyword(s):  
Nicotinic Acid ◽  
G Protein ◽  
G Protein Coupled Receptor ◽  
G Protein Coupled

Characterization of G-Protein Coupled Receptor Modulators Using Homogeneous cAMP Assays

2012 ◽  
pp. 171-180 ◽  
Author(s):  
Daniel L. Bassoni ◽  
Qumber Jafri ◽  
Sunitha Sastry ◽  
Mahesh Mathrubutham ◽  
Tom S. Wehrman
Keyword(s):  
G Protein ◽  
G Protein Coupled Receptor ◽  
Receptor Modulators ◽  
G Protein Coupled

Effects ofN- andC-terminal addition of oligolysines or native loop residues on the biophysical properties of transmembrane domain peptides from a G-protein coupled receptor

2006 ◽  
Vol 12 (12) ◽  
pp. 808-822 ◽  
Author(s):  
Patricia Cano-Sanchez ◽  
Beatrice Severino ◽  
V. V. Sureshbabu ◽  
Joe Russo ◽  
Tatsuya Inui ◽  
...  
Keyword(s):  
G Protein ◽  
Transmembrane Domain ◽  
Biophysical Properties ◽  
G Protein Coupled Receptor ◽  
G Protein Coupled

scholarly journals Expression and biophysical analysis of two double-transmembrane domain-containing fragments from a yeast G protein-coupled receptor

Biopolymers ◽  
2008 ◽  
Vol 90 (2) ◽  
pp. 117-130 ◽  
Author(s):  
Leah S. Cohen ◽  
Boris Arshava ◽  
Racha Estephan ◽  
Jacqueline Englander ◽  
Heejung Kim ◽  
...  
Keyword(s):  
G Protein ◽  
Transmembrane Domain ◽  
G Protein Coupled Receptor ◽  
Biophysical Analysis ◽  
G Protein Coupled
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