In vitromodels for metabolic studies of small peptide hormones in sport drug testing

2014 ◽  
Vol 21 (1) ◽  
pp. 1-9 ◽  
Author(s):  
Simone Esposito ◽  
Koen Deventer ◽  
Lore Geldof ◽  
Peter Van Eenoo
Keyword(s):  
Drug Testing ◽  
Peptide Hormones ◽  
Small Peptide ◽  
Metabolic Studies ◽  
Sport Drug Testing

Chimeric strategies for the rational design of bioactive analogs of small peptide hormones

1997 ◽  
Vol 11 (7) ◽  
pp. 582-591 ◽  
Author(s):  
John Howl ◽  
ülo Langel ◽  
Stuart R. Hawtin ◽  
Andres Valkna ◽  
Nicola J. Yarwood ◽  
...  
Keyword(s):  
Rational Design ◽  
Peptide Hormones ◽  
Small Peptide

scholarly journals Plant Protein O-Arabinosylation

2021 ◽  
Vol 12 ◽  
Author(s):  
Bent Larsen Petersen ◽  
Cora A. MacAlister ◽  
Peter Ulvskov
Keyword(s):  
Cell Wall ◽  
Stress Responses ◽  
Secretory Pathway ◽  
Peptide Hormones ◽  
Common Denominator ◽  
Small Peptide ◽  
Structural Cell ◽  
Additional Layer ◽  
Wide Range ◽  
Diverse Groups

A wide range of proteins with diverse functions in development, defense, and stress responses areO-arabinosylated at hydroxyprolines (Hyps) within distinct amino acid motifs of continuous stretches of Hyps, as found in the structural cell wall extensins, or at non-continuous Hyps as, for example, found in small peptide hormones and a variety of plasma membrane proteins involved in signaling. PlantO-glycosylation relies on hydroxylation of Prolines to Hyps in the protein backbone, mediated by prolyl-4-hydroxylase (P4H) which is followed byO-glycosylation of the Hyp C4-OH group by either galactosyltransferases (GalTs) or arabinofuranosyltranferases (ArafTs) yielding either Hyp-galactosylation or Hyp-arabinosylation. A subset of the P4H enzymes with putative preference to hydroxylation of continuous prolines and presumably all ArafT enzymes needed for synthesis of the substituted arabinose chains of one to four arabinose units, have been identified and functionally characterized. Truncated root-hair phenotype is one common denominator of mutants of Hyp formation and Hyp-arabinosylation glycogenes, which act on diverse groups ofO-glycosylated proteins, e.g., the small peptide hormones and cell wall extensins. Dissection of different substrate derived effects may not be regularly feasible and thus complicate translation from genotype to phenotype. Recently, lack of proper arabinosylation on arabinosylated proteins has been shown to influence their transport/fate in the secretory pathway, hinting to an additional layer of functionality ofO-arabinosylation. Here, we provide an update on the prevalence and types ofO-arabinosylated proteins and the enzymatic machinery responsible for their modifications.

scholarly journals Detection of Small Peptide Hormones for Anti-Doping Purpose via a Molecularly Imprinted Polymer-Based SPR Assay

Proceedings ◽  
2020 ◽  
Vol 60 (1) ◽  
pp. 56
Author(s):  
Francesca Torrini ◽  
Pasquale Palladino ◽  
Simona Scarano ◽  
Maria Minunni
Keyword(s):  
Molecularly Imprinted Polymer ◽  
Bioanalytical Methods ◽  
Low Cost ◽  
Peptide Hormones ◽  
Imprinted Polymer ◽  
Small Peptide ◽  
Molecularly Imprinted

Currently, there is a lack of low-cost, prompt and robust bioanalytical methods to detect smallpeptide hormones (e.g., gonadorelin, buserelin, leuprorelin, etc.) in the routine anti-doping protocol. [...]

Metabolism of steroids and sport drug testing

Bioanalysis ◽  
2020 ◽  
Vol 12 (9) ◽  
pp. 561-563
Author(s):  
Biljana Jancic Stojanovic ◽  
Lorenz Göschl ◽  
Guro Forsdahl ◽  
Gmeiner Günter
Keyword(s):  
Drug Testing ◽  
Sport Drug Testing
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