Tribenuron‐methyl‐resistant Descurainia sophia L. exhibits negative cross‐resistance to imazethapyr conferred by a Pro197Ser mutation in acetolactate synthase ( ALS ) and reduced metabolism

2021 ◽  
Author(s):  
Yufang Xu ◽  
Shuang Li ◽  
Lubo Hao ◽  
Xuefeng Li ◽  
Mingqi Zheng
Keyword(s):  
Acetolactate Synthase ◽  
Cross Resistance ◽  
Descurainia Sophia ◽  
Tribenuron Methyl

scholarly journals Effects of a novel combination of two mutated acetolactate synthase (ALS) isozymes on resistance to ALS-inhibiting herbicides in Flixweed (Descurainia Sophia)

Weed Science ◽  
2021 ◽  
pp. 1-36
Author(s):  
Yufang Xu ◽  
Lina Xu ◽  
Jing Shen ◽  
Xuefeng Li ◽  
Mingqi Zheng
Keyword(s):  
Resistance Management ◽  
Acetolactate Synthase ◽  
Expression Level ◽  
Cross Resistance ◽  
Resistance Mutations ◽  
Relative Expression ◽  
Chemical Structures ◽  
Descurainia Sophia ◽  
Relative Expression Level ◽  
Tribenuron Methyl

Abstract Flixweed [Descurainia sophia (L.) Webb ex Prantl] is a notorious broadleaf weed that is widely distributed in winter wheat growing areas of China, and has evolved resistance to tribenuron-methyl mainly due to target-site resistance (TSR) mutations in acetolactate synthase (ALS). In the current research, two ALS genes were identified in tribenuron-methyl-susceptible (TS) or -resistant (TR) D. sophia. Resistance mutations of Asp-376-Glu and Pro-197-Ala were identified on ALS1 and ALS2 isozymes in TR D. sophia, respectively. The TR D. sophia evolved 10836.3-fold resistance to tribenuron-methyl and displayed cross-resistance to multiple ALS-inhibiting herbicides with different chemical structures. Dose response experiments and ALS activity assay indicated that two mutated ALS isozymes contributed differentially in resistance to tribenuron-methyl, flucetosulfuron and pyribenzoxim. In addition, the relative expression level of ALS1 gene was 2.2- and 1.6-fold higher than ALS2 genes in TR D. sophia on the 1st and 7th days after tribenuron-methyl treatment (DAT), respectively. In contrast, the relative expression level of ALS1 and ALS2 in TS D. sophia is similar. This is the first research that explored different roles of ALS isozymes in resistance to ALS-inhibiting herbicides, which might provide a new perspective for the weed resistance management.

Continuous Use of Tribenuron-Methyl Selected for Cross-Resistance to Acetolactate Synthase–inhibiting Herbicides in Wild Mustard (Sinapis arvensis)

Weed Science ◽  
2018 ◽  
Vol 66 (4) ◽  
pp. 424-432 ◽  
Author(s):  
Javid Gherekhloo ◽  
Zahra M. Hatami ◽  
Ricardo Alcántara-de la Cruz ◽  
Hamid R. Sadeghipour ◽  
Rafael De Prado
Keyword(s):  
Winter Wheat ◽  
Acetolactate Synthase ◽  
Dry Weight ◽  
Resistance Mechanisms ◽  
Cross Resistance ◽  
Resistance Level ◽  
Sinapis Arvensis ◽  
Wild Mustard ◽  
Golestan Province ◽  
Tribenuron Methyl

AbstractWild mustard (Sinapis arvensis L.) is a weed that frequently infests winter wheat (Triticum aestivum L.) fields in Golestan province, Iran. Tribenuron-methyl (TM) has been used recurrently to control this species, thus selecting for resistant S. arvensis populations. The objectives were: (1) to determine the resistance level to TM of 14 putatively resistant (PR) S. arvensis populations, collected from winter wheat fields in Golestan province, Iran, in comparison to one susceptible (S) population; and (2) to characterize the resistance mechanisms and the potential evolution of cross-resistance to other classes of acetolactate synthase (ALS)-inhibiting herbicides in three populations (AL-3, G-5, and Ag-Sr) confirmed as being resistant (R) to TM. The TM doses required to reduce the dry weight of the PR populations by 50% were between 2.2 and 16.8 times higher than those needed for S plants. The ALS enzyme activity assays revealed that the AL-3, G-5, and Ag-Sr populations evolved cross-resistance to the candidate ALS-inhibiting herbicides from the sulfonylureas (SU), triazolopyrimidines (TP), pyrimidinyl-thiobenzoates (PTB), sulfonyl-aminocarbonyl-triazolinone (SCT), and imidazolinones (IMI) classes. No differences in absorption, translocation, or metabolism of [14C]TM between R and S plants were observed, suggesting that these non-target mechanisms were not responsible for the resistance. The ALS gene of the R populations contained the Trp-574-Leu mutation, conferring cross-resistance to the SU, SCT, PTB, TP, and IMI classes. The Trp-574-Leu mutation in the ALS gene conferred cross-resistance to ALS-inhibiting herbicides in S. arvensis from winter wheat fields in Golestan province. This is the first TM resistance case confirmed in this species in Iran.

scholarly journals Characterization of resistance and fitness cost of Descurainia sophia L. populations from Henan and Xinjiang, China

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Dongzhi Li ◽  
Lanfen Xie ◽  
Pei Zhang ◽  
Runqiang Liu ◽  
Mingwang Shi ◽  
...  
Keyword(s):  
Fitness Cost ◽  
Cross Resistance ◽  
Homozygous Mutation ◽  
Susceptible Population ◽  
Relative Growth Rates ◽  
Wheat Field ◽  
Production Region ◽  
Descurainia Sophia ◽  
Mutation Type ◽  
Tribenuron Methyl

AbstractDescurainia sophia L. is a notorious weed in winter wheat field and has serious resistance to tribenuron-methyl. Xinjiang is a main wheat production region in China with no information on D. sophia resistance to tribenuron-methyl. Here, resistance levels of D. sophia populations to tribenuron-methyl from Xinjiang and Henan were investigated. In addition, homozygous mutation subpopulations of high resistant D. sophia populations from Xinjiang and Henan were generated and then cross-resistance and fitness cost were determined. Results showed that 5 out of 31 populations from Xinjiang developed resistance to tribenuron-methyl, including two high resistant populations (X30 and X31). While 10 out of 11 populations from Henan showed resistance to tribenuron-methyl, including three high resistant populations (H5, H6 and H7). X30 and X31 shared the same mutation type of Pro197Thr in ALS1, while the mutation type of ALS1 in H5, H6 and H7 were Pro197Ser, Pro197His and Pro197Ala, respectively. The homozygous mutation subpopulations (SX30, SX31, SH5, SH6, SH7) showed cross-resistance to flucarbazone-sodium, bensulfuron methyl and flumetsulam. Under monoculture condition, relative growth rates of SX30, SX31 were higher than susceptible population (SX13), while that in SH5, SH6, SH7 were almost same with SX13. When mix planted with SX13, SX30 and SX31 displayed weaker competitiveness than SX13, while SH5, SH6, SH7 showed stronger competitiveness than SX13. The results suggested that D. sophia from Xinjiang had low resistance frequency to tribenuron-methyl and the high resistant populations had fitness costs.

Target-Site Resistance Mechanisms to Tribenuron-methyl and Cross-resistance Patterns to ALS-inhibiting Herbicides of Catchweed Bedstraw (Galium aparine) with Different ALS Mutations

Weed Science ◽  
2018 ◽  
Vol 67 (2) ◽  
pp. 183-188 ◽  
Author(s):  
Wei Deng ◽  
Yingjie Di ◽  
Jingxuan Cai ◽  
Yueyang Chen ◽  
Shuzhong Yuan
Keyword(s):  
Acetolactate Synthase ◽  
Resistance Mechanisms ◽  
Target Site ◽  
Cross Resistance ◽  
Susceptible Population ◽  
Resistance Evolution ◽  
Galium Aparine ◽  
Resistance Patterns ◽  
Target Site Resistance ◽  
Tribenuron Methyl

AbstractCatchweed bedstraw (Galium aparine L.) is a problematic dicot weed that occurs in major winter wheat (Triticum aestivum L.) fields in China. Tribenuron-methyl has been widely used to control broadleaf weeds since 1988 in China. However, overuse has led to the resistance evolution of G. aparine to tribenuron-methyl. In this study, 20 G. aparine populations collected from Shandong and Henan provinces were used to determine tribenuron-methyl resistance and target-site resistance mechanisms. In dose–response experiments, 12 G. aparine populations showed different resistance levels (2.92 to 842.41-fold) to tribenuron-methyl compared with the susceptible population. Five different acetolactate synthase (ALS) mutations (Pro-197-Leu, Pro-197-Ser, Pro-197-His, Asp-376-Glu, and Trp-574-Leu) were detected in different resistant populations. Individuals heterozygous for Pro-197-Ser and Trp-574-Leu mutations were also observed in a resistant population (HN6). In addition, pHB4 (Pro-197-Ser), pHB7 (Pro-197-His), pHB8 (Pro-197-Leu), pHB5 (Asp-376-Glu), and pHB3 (Trp-574-Leu) subpopulations individually homozygous for specific ALS mutations were generated to evaluate the cross-resistance to ALS-inhibiting herbicides. The pHB4, pHB7, pHB8, pHB5, and pHB3 subpopulations all were resistant to sulfonylurea, pyrazosulfuron-ethyl, triazolopyrimidine, flumetsulam, sulfonylamino-carbonyl-triazolinone, flucarbazone-sodium, pyrimidinyl thiobenzoate, pyribenzoxim, and the imidazolinone imazethapyr. These results indicated the diversity of the resistance-conferring ALS mutations in G. aparine, and all these mutations resulted in broad cross-resistance to five kinds of ALS-inhibiting herbicides.

Molecular basis of resistance to tribenuron-methyl in Descurainia Sophia (L.) populations from China

2012 ◽  
Vol 104 (1) ◽  
pp. 77-81 ◽  
Author(s):  
Xue jing Han ◽  
Yi Dong ◽  
Xiao na Sun ◽  
Xue feng Li ◽  
Ming qi Zheng
Keyword(s):  
Molecular Basis ◽  
Descurainia Sophia ◽  
Tribenuron Methyl
Sign in / Sign up

Export Citation Format

Share Document