Suppression of Bta11975 , an α‐glucosidase , by RNA interference reduces transmission of Tomato chlorosis virus by Bemisia tabaci

2021 ◽  
Author(s):  
Ding Yi Hui Lu ◽  
Hao Yue ◽  
Li Ping Huang ◽  
De Yong Zhang ◽  
Zhan Hong Zhang ◽  
...  
Keyword(s):  
Rna Interference ◽  
Bemisia Tabaci ◽  
Tomato Chlorosis Virus

Host Range Studies for Tomato chlorosis virus, and Cucumber vein yellowing virus Transmitted by Bemisia  tabaci (Gennadius)

2006 ◽  
Vol 114 (3) ◽  
pp. 265-273 ◽  
Author(s):  
Jane Morris ◽  
Elspeth Steel ◽  
Penny Smith ◽  
Neil Boonham ◽  
Nicola Spence ◽  
...  
Keyword(s):  
Host Range ◽  
Bemisia Tabaci ◽  
Tomato Chlorosis Virus ◽  
Yellowing Virus

scholarly journals Two Deoxythymidine Triphosphate Synthesis-Related Genes Regulate Obligate Symbiont Density and Reproduction in the Whitefly Bemisia tabaci MED

2021 ◽  
Vol 11 ◽  
Author(s):  
Zezhong Yang ◽  
Cheng Gong ◽  
Yuan Hu ◽  
Jie Zhong ◽  
Jixing Xia ◽  
...  
Keyword(s):  
Amino Acid ◽  
Rna Interference ◽  
Bemisia Tabaci ◽  
Essential Amino Acid ◽  
Cellular Growth ◽  
Capacity Analysis ◽  
Host Insect ◽  
Obligate Symbiont ◽  
Pyrimidine Pathway

Deoxythymidine triphosphate (dTTP) is essential for DNA synthesis and cellular growth in all organisms. Here, genetic capacity analysis of the pyrimidine pathway in insects and their symbionts revealed that dTTP is a kind of metabolic input in several host insect/obligate symbiont symbiosis systems, including Bemisia tabaci MED/Candidatus Portiera aleyrodidarum (hereafter Portiera). As such, the roles of dTTP on both sides of the symbiosis system were investigated in B. tabaci MED/Portiera. Dietary RNA interference (RNAi) showed that suppressing dTTP production significantly reduced the density of Portiera, significantly repressed the expression levels of horizontally transferred essential amino acid (EAA) synthesis-related genes, and significantly decreased the reproduction of B. tabaci MED adults as well as the hatchability of their offspring. Our results revealed the regulatory role of dTTP in B. tabaci MED/Portiera and showed that dTTP synthesis-related genes could be potential targets for controlling B. tabaci as well as other sucking pests.

Inaccessibility to double‐stranded RNAs in plastids restricts RNA interference in Bemisia tabaci (whitefly)

2020 ◽  
Vol 76 (9) ◽  
pp. 3168-3176 ◽  
Author(s):  
Yi Dong ◽  
Yong Yang ◽  
Zican Wang ◽  
Mengting Wu ◽  
Jinqiu Fu ◽  
...  
Keyword(s):  
Rna Interference ◽  
Bemisia Tabaci

scholarly journals RNAi-Mediated Management of Whitefly Bemisia tabaci by Oral Delivery of Double-stranded RNAs

Proceedings ◽  
2019 ◽  
Vol 36 (1) ◽  
pp. 11
Author(s):  
Jain ◽  
Robinson ◽  
Mitter
Keyword(s):  
Rna Interference ◽  
Gene Silencing ◽  
Bemisia Tabaci ◽  
Oral Delivery ◽  
Target Genes ◽  
Insect Pests ◽  
Control Strategies ◽  
Plant Viruses ◽  
Potential Game ◽  
Viable Approach

The whitefly, Bemisia tabaci (Hemiptera: Aleyrodidae) is a significant global pest of economically important vegetable, fibre, and ornamental crops. Whiteflies directly damage the plants by piercing and sucking essential nutrients, indirectly through honeydew secretion and by transmitting more than 200 plant viruses that cause millions of dollars in produce losses per year. Whitefly management is mostly reliant on the heavy use of chemical insecticides. However, this ultimately leads to increasing resistance development, detrimental effects on beneficial insects and biomagnification of ecologically harmful chemicals in the environment. Responding to consumer demands for more selective, less toxic, non-GM insect control strategies, RNA interference (RNAi) has emerged as a potential game-changing solution. The RNA interference (RNAi) is a homology-dependent mechanism of gene silencing that represents a feasible and sustainable technology for the management of insect pests. In the present study, twenty-two whitefly genes were selected based on their essential function in the insect and tested in artificial diet bioassays for mortality and gene silencing efficacy. The nine most effective dsRNA constructs showed moderate-to-high whitefly mortality as compared to negative controls six days post-feeding. qPCR analysis further demonstrated significant knockdown of target gene mRNA expression. Additionally, uptake and spread of fluorescently labelled dsRNA was evident beyond the midgut of the whitefly supporting the systemic spreading of RNAi effectors. Taken together, the oral delivery of dsRNA shows effective RNAi mediated gene silencing of target genes and offers a viable approach for the development of dsRNA biopesticides against hemipteran pest.

Genome‐wide identification and analysis of genes associated with RNA interference in Bemisia tabaci

2019 ◽  
Vol 75 (11) ◽  
pp. 3005-3014 ◽  
Author(s):  
Lixia Tian ◽  
Yang Zeng ◽  
Wen Xie ◽  
Qingjun Wu ◽  
Shaoli Wang ◽  
...  
Keyword(s):  
Rna Interference ◽  
Bemisia Tabaci ◽  
Genome Wide

scholarly journals RNA interference-mediated tolerance to whitefly (Bemisia tabaci) in genetically engineered tomato

2021 ◽  
Author(s):  
Carolina Senhorinho Ramalho Pizetta ◽  
William Rafael Ribeiro ◽  
Amanda Lopes Ferreira ◽  
Matheus da Costa Moura ◽  
Kenny Bonfim ◽  
...  
Keyword(s):  
Rna Interference ◽  
Transgenic Plants ◽  
Bemisia Tabaci ◽  
Insect Pest ◽  
Genetically Engineered ◽  
Horticultural Crops ◽  
Transgenic Tomato Plants ◽  
Atpase Gene ◽  
Transgenic Lines ◽  
Polyphagous Insect

Abstract Whitefly (Bemisia tabaci) is a polyphagous insect that causes huge damage in several horticultural crops, including tomato, by sucking nutrients from the phloem and transmitting viruses. Whiteflies are particularly difficult to manage and the use of chemicals remains the common practice, which causes the development of insecticidal resistance. Thus, there is considerable interest in the introduction of whitefly resistance by classical and molecular breeding. Here, we explored the concept of using an RNA interference construct to silence a v-ATPase gene in whiteflies interacting with transgenic tomato plants that express siRNA molecules corresponding to a fragment from the B. tabaci vATPase. PCR analyses revealed the presence of both ΔATPase and nptII transgenes in all transgenic lines. siRNA expressing lines were challenged against whitefly and revealed a mortality rate of 57.1% in transgenic line 4.4.1, while in the control the mortality was 7.6%. Mortality of 2nd instar nymphs was higher on the transgenic plants and the development of 3rd instar nymphs was slightly longer than on the control plants. Although the attraction of insects was not significantly different between treatments, the number of eggs laid by the insects on the transgenic plants was significantly lower, compared to the controls. RT-qPCR revealed a decreased expression level of endogenous v-ATPase gene in whiteflies feeding on transgenic plants. No unexpected effect was observed on the non-target insects Myzus persicae or Tuta absoluta. Results presented here may form the foundation for the generation of elite tomato varieties resistant to whitefly, a devastating insect pest.

scholarly journals Improved efficacy of neonicotinoid in tablet formulation on the control of tomato chlorosis virus by controlling the vector Bemisia tabaci

2020 ◽  
Vol 2 (1) ◽  
Author(s):  
Lei Li ◽  
Zhihong Hu ◽  
Tan Dai ◽  
Panqing Liu ◽  
Chen Chen ◽  
...  
Keyword(s):  
Bemisia Tabaci ◽  
Environmental Contamination ◽  
Foliar Spray ◽  
Plant Viruses ◽  
Tablet Formulation ◽  
Soil Treatment ◽  
In Vitro Test ◽  
Sustained Release Tablets ◽  
Tomato Chlorosis Virus

AbstractNeonicotinoid insecticides are used for preventing insects from transmitting plant viruses. This group of chemicals are easily taken up by plants and translocated to different tissues and are applicable for soil treatment in sustained-release tablets, which greatly reduces environmental contamination compared to foliar spray. The goal of this study was to examine the efficacy of thiamethoxam, acetamiprid and nitenpyram in tablet formulation against whitefly, Bemisia tabaci, the vector of tomato chlorosis virus (ToCV) under laboratory and greenhouse conditions. Effective 50% lethal concentration (LC50) of thiamethoxam, acetamiprid, and nitenpyram on B. tabaci were 2.18, 0.46 and 0.18 μg/g plant tissue, respectively. In vitro test showed that tablets of 10 mg nitenpyram applied in seedbed with thiamethoxam transplanting treatment showed 80.9% control of B. tabaci on tomato and 75.5% of ToCV after 32 days of transplanting. Therefore, neonicotinoid tablets were an effective strategy of soil treatment in controlling viruliferous insects and ToCV with reduced environmental contamination.

Natural Infection Rate of Known Tomato Chlorosis Virus-Susceptible Hosts and the Influence of the Host Plant on the Virus Relationship With Bemisia tabaci MEAM1

Plant Disease ◽  
2020 ◽  
Author(s):  
Arnaldo Esquivel-Fariña ◽  
Jorge Alberto Marques Rezende ◽  
William M. Wintermantel ◽  
Laura Jenkins Hladky ◽  
Daiana Bampi
Keyword(s):  
Infection Rate ◽  
Bemisia Tabaci ◽  
Host Plants ◽  
Natural Infection ◽  
Sao Paulo ◽  
Cultivated Plants ◽  
São Paulo ◽  
Susceptible Host ◽  
External Sources ◽  
Tomato Chlorosis Virus

Tomato chlorosis virus (ToCV; genus Crinivirus, family Closteroviridae) was identified in tomato crops in the state of São Paulo, Brazil in 2006. Management strategies to control external sources of inoculum are necessary, because chemical control of the whitefly vector Bemisia tabaci MEAM1 has not efficiently prevented virus infections and no commercial tomato cultivars or hybrids are resistant to this crinivirus. We first evaluated the natural infection rate of some known wild and cultivated ToCV-susceptible hosts and their attractiveness for B. tabaci MEAM1 oviposition. Physalis angulata was the most susceptible to natural infection in all six exposures in 2018 and 2019. No plants of Capsicum annuum (cv. Dahra) or Chenopodium album became infected. Solanum melongena (cv. Napoli) had only two infected plants of 60 exposed. C. annuum and C. album were the least preferred, and Nicotiana tabacum and S. melongena were the most preferred for whitefly oviposition. In addition, from 2016 to 2019, we surveyed different tomato crops and the surrounding vegetation to identify ToCV in weeds and cultivated plants in the region of Sumaré, São Paulo state. Only Solanum americanum, vila vila (S. sisymbriifolium) and C. album were found naturally infected, with incidences of 18%, 20% and 1.4%, respectively. Finally, we estimated the ToCV titer (isolates ToCV-FL, USA and ToCV-SP, Brazil) by RT-qPCR in different ToCV-susceptible host plants and evaluated the relationship between virus acquisition and transmission by B. tabaci MEAM1. The results clearly showed significant differences in ToCV concentrations in the tissues of ToCV-susceptible host plants, which appeared to be influenced by the virus isolate. The concentration of the virus in plant tissues, in turn, directly influenced the ToCV-B. tabaci MEAM1 relationship and subsequent transmission to tomato plants. To minimize or prevent the damage from the tomato yellowing disease through management of external sources of ToCV, it is necessary to correctly identify the potentially important ToCV-susceptible hosts in the vicinity of new plantings.

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