Comprehensive insights into host‐pathogen interaction between brown planthopper and a fungal entomopathogen by dual RNA sequencing

2021 ◽  
Author(s):  
Zheng‐Liang Wang ◽  
Hai‐Bo Pan ◽  
Mu‐Yu Li ◽  
Wei Wu ◽  
Xiao‐Ping Yu
Keyword(s):  
Rna Sequencing ◽  
Brown Planthopper ◽  
Host Pathogen Interaction ◽  
Host Pathogen ◽  
Fungal Entomopathogen

scholarly journals Assessing Host-Pathogen Interaction Networks via RNA-Seq Profiling: A Systems Biology Approach

2021 ◽  
Author(s):  
Sudhesh Dev Sareshma ◽  
Bhassu Subha
Keyword(s):  
Rna Sequencing ◽  
High Throughput Sequencing ◽  
Ribosome Profiling ◽  
Host Pathogen Interaction ◽  
Host Pathogen ◽  
Experimental Approaches ◽  
The Common ◽  
Next Generation Sequencing Ngs ◽  
Potential Use ◽  
Generation Sequencing

RNA sequencing is a valuable tool brought about by advances in next generation sequencing (NGS) technology. Initially used for transcriptome mapping, it has grown to become one of the ‘gold standards’ for studying molecular changes that occur in niche environments or within and across infections. It employs high-throughput sequencing with many advantages over previous methods. In this chapter, we review the experimental approaches of RNA sequencing from isolating samples all the way to data analysis methods. We focus on a number of NGS platforms that offer RNA sequencing with each having their own strengths and drawbacks. The focus will also be on how RNA sequencing has led to developments in the field of host-pathogen interactions using the dual RNA sequencing technique. Besides dual RNA sequencing, this review also explores the application of other RNA sequencing techniques such as single cell RNA sequencing as well as the potential use of newer techniques like ‘spatialomics’ and ribosome-profiling in host-pathogen interaction studies. Finally, we examine the common challenges faced when using RNA sequencing and possible ways to overcome these challenges.

scholarly journals The Role of Macrophages in the Host’s Defense against Sporothrix schenckii

Pathogens ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 905
Author(s):  
Estela Ruiz-Baca ◽  
Armando Pérez-Torres ◽  
Yolanda Romo-Lozano ◽  
Daniel Cervantes-García ◽  
Carlos A. Alba-Fierro ◽  
...  
Keyword(s):  
Immune Responses ◽  
Macrophage Polarization ◽  
Sporothrix Schenckii ◽  
Macrophage Cell ◽  
Cell Recruitment ◽  
Host Pathogen Interaction ◽  
Interaction Processes ◽  
Host Pathogen ◽  
Molecular Patterns

The role of immune cells associated with sporotrichosis caused by Sporothrix schenckii is not yet fully clarified. Macrophages through pattern recognition receptors (PRRs) can recognize pathogen-associated molecular patterns (PAMPs) of Sporothrix, engulf it, activate respiratory burst, and secrete pro-inflammatory or anti-inflammatory biological mediators to control infection. It is important to consider that the characteristics associated with S. schenckii and/or the host may influence macrophage polarization (M1/M2), cell recruitment, and the type of immune response (1, 2, and 17). Currently, with the use of new monocyte-macrophage cell lines, it is possible to evaluate different host–pathogen interaction processes, which allows for the proposal of new mechanisms in human sporotrichosis. Therefore, in order to contribute to the understanding of these host–pathogen interactions, the aim of this review is to summarize and discuss the immune responses induced by macrophage-S. schenckii interactions, as well as the PRRs and PAMPs involved during the recognition of S. schenckii that favor the immune evasion by the fungus.

scholarly journals Host–Pathogen Interaction in Invasive Salmonellosis

2012 ◽  
Vol 8 (10) ◽  
pp. e1002933 ◽  
Author(s):  
Hanna K. de Jong ◽  
Chris M. Parry ◽  
Tom van der Poll ◽  
W. Joost Wiersinga
Keyword(s):  
Host Pathogen Interaction ◽  
Host Pathogen

scholarly journals Transcriptional response ofNautella italicaR11 towards its macroalgal host uncovers new mechanisms of host-pathogen interaction

2017 ◽  
Vol 27 (8) ◽  
pp. 1820-1832 ◽  
Author(s):  
Jennifer Hudson ◽  
Melissa Gardiner ◽  
Nandan Deshpande ◽  
Suhelen Egan
Keyword(s):  
Transcriptional Response ◽  
Host Pathogen Interaction ◽  
Host Pathogen
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