scholarly journals Sampling pollen beetle ( Brassicogethes aeneus ) pressure in oilseed rape: which method is best?

2021 ◽  
Author(s):  
Gaëtan Seimandi‐Corda ◽  
Todd Jenkins ◽  
Samantha M Cook
Keyword(s):  
Oilseed Rape ◽  
Pollen Beetle ◽  
Brassicogethes Aeneus

scholarly journals RNAi efficacy is enhanced by chronic dsRNA feeding in pollen beetle

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Jonathan Willow ◽  
Liina Soonvald ◽  
Silva Sulg ◽  
Riina Kaasik ◽  
Ana Isabel Silva ◽  
...  
Keyword(s):  
Rna Interference ◽  
Pest Management ◽  
Oilseed Rape ◽  
Growth Stages ◽  
Short Term ◽  
Pollen Beetle ◽  
Brassicogethes Aeneus ◽  
Crop Growth Stages ◽  
Insecticidal Compounds ◽  
Significant Mortality

AbstractDouble-stranded RNAs (dsRNAs) represent a promising class of biosafe insecticidal compounds. We examined the ability to induce RNA interference (RNAi) in the pollen beetle Brassicogethes aeneus via anther feeding, and compared short-term (3 d) to chronic (17 d) feeding of various concentrations of dsRNA targeting αCOP (dsαCOP). In short-term dsαCOP feeding, only the highest concentration resulted in significant reductions in B. aeneus survival; whereas in chronic dsαCOP feeding, all three concentrations resulted in significant mortality. Chronic dsαCOP feeding also resulted in significantly greater mortality compared to short-term feeding of equivalent dsαCOP concentrations. Our results have implications for the economics and development of dsRNA spray approaches for managing crop pests, in that multiple lower-concentration dsRNA spray treatments across crop growth stages may result in greater pest management efficacy, compared to single treatments using higher dsRNA concentrations. Furthermore, our results highlight the need for research into the development of RNAi cultivars for oilseed rape protection, given the enhanced RNAi efficacy resulting from chronic, compared to short-term, dsRNA feeding in B. aeneus.

scholarly journals Characterization of the pollen beetle, Brassicogethes aeneus, dispersal from woodlands to winter oilseed rape fields

PLoS ONE ◽  
2017 ◽  
Vol 12 (8) ◽  
pp. e0183878 ◽  
Author(s):  
Amandine Suzanne Juhel ◽  
Corentin Mario Barbu ◽  
Pierre Franck ◽  
Jean Roger-Estrade ◽  
Arnaud Butier ◽  
...  
Keyword(s):  
Oilseed Rape ◽  
Winter Oilseed Rape ◽  
Pollen Beetle ◽  
Brassicogethes Aeneus

scholarly journals Spatio-temporal Distributions and Associations of Cabbage Stem Weevil (Ceutorhynchus Pallidactylus Marsham, 1802) and Pollen Beetle (Brassicogethes Aeneus Fabricius, 1775) in winter oilseed rape

2017 ◽  
Vol 65 (3) ◽  
pp. 839-847
Author(s):  
Vojtěch Hlavjenka ◽  
Marek Seidenglanz ◽  
Jaroslav Šafář
Keyword(s):  
Oilseed Rape ◽  
Cumulative Distribution ◽  
Winter Oilseed Rape ◽  
High Tendency ◽  
Pollen Beetle ◽  
Brassicogethes Aeneus ◽  
Males And Females ◽  
Spatio Temporal ◽  
The Individual ◽  
Ceutorhynchus Pallidactylus

From 2013 to 2015 the within-field spatio-temporal distributions and interactions between Ceutorhynchus pallidactylus and Brassicogethes aeneus in winter oilseed rape crops were assessed using SADIE analysis (Spatial Analysis by Distance IndicEs) and Quick association analysis. A significant tendency for aggregation was recorded in C. pallidactylus males in the crop in both 2013 and 2015. Females showed a high tendency towards aggregation only in 2013. Both sexes were significantly associated in crops in 2013 and 2015. B. aeneus adults showed a high tendency towards aggregation in all three years. From the three seasons the cumulative distributions (based on the sums of insect catches in traps for the individual dates) of males and females of C. pallidactylus were significantly associated with the cumulative distribution of B. aeneus adults only in 2013. Contrary to this, an almost significant dissociation between cumulative distributions of C. pallidactylus males and B. aeneus adults was recorded in 2014.

scholarly journals Attractiveness of oilseed rape cultivars to Brassicogethes aeneus and Ceutorhynchus obstrictus as a potential control strategy  

2021 ◽  
Author(s):  
Tomáš Hovorka ◽  
Pavel Saska ◽  
Jitka Stará ◽  
František Kocourek
Keyword(s):  
Oilseed Rape ◽  
Control Strategy ◽  
Negative Impact ◽  
Pollen Beetle ◽  
Brassicogethes Aeneus ◽  
Potential Control ◽  
Ceutorhynchus Obstrictus ◽  
Cabbage Seed Weevil ◽  
Cabbage Seed ◽  
The One

The abundances of two pests, pollen beetle (Brassicogethes aeneus (Fabricius, 1775)) and cabbage seed weevil (Ceutorhynchus obstrictus (Marsham, 1802)), were measured before flowering and in the full bloom of oilseed rape cultivars with different phenologies (two yellow-flowering: DK Exssence (the earliest), DK Sensei (the latest) and one white-flowering in time between yellow-flowering cultivars), and the differences in their abundance in the selected cultivars were determined in plot experiments during 2015–2018. No significant differences in pollen beetle and cabbage seed weevil occurrence were observed between the cultivars in the pre-flowering period, but during flowering, the two yellow-flowering cultivars were more attractive than the white-flowering cultivar for both pests. In the white-flowering cultivar, 57% and 69% reductions in the pollen beetle and cabbage seed weevil populations, respectively, were found relative to the two with yellow flowers. Thus, the use of white-flowering cultivar (less attractive, later flowering) as the main crop and the yellow-flowering cultivar (more attractive, earlier flowering) at field edges, with the width of the one-track line, could serve as a strategy to manage oilseed rape pests during flowering. This control strategy which combines more and less attractive oilseed rape cultivars may contribute to a reduction in the use of pesticides and their negative impact on the environment.  

scholarly journals First Evidence of Bud Feeding-Induced RNAi in a Crop Pest via Exogenous Application of dsRNA

Insects ◽  
2020 ◽  
Vol 11 (11) ◽  
pp. 769
Author(s):  
Jonathan Willow ◽  
Liina Soonvald ◽  
Silva Sulg ◽  
Riina Kaasik ◽  
Ana Isabel Silva ◽  
...  
Keyword(s):  
Nucleotide Sequence ◽  
Gene Silencing ◽  
Oilseed Rape ◽  
Dietary Exposure ◽  
Exposure Period ◽  
Sequence Specificity ◽  
Pollen Beetle ◽  
Brassicogethes Aeneus ◽  
Model Control ◽  
Potential Strategy

Spray-induced gene silencing (SIGS) is a potential strategy for agricultural pest management, whereby nucleotide sequence-specific double-stranded RNA (dsRNA) can be sprayed onto a crop; the desired effect being a consumption of dsRNA by the target pest, and subsequent gene silencing-induced mortality. Nucleotide sequence-specificity is the basis for dsRNA’s perceived biosafety. A biosafe approach to pollen beetle (Brassicogethes aeneus) management in oilseed rape (Brassica napus) agroecosystems is needed. We examined the potential for SIGS in B. aeneus, via bud feeding, a field-relevant dsRNA exposure route. Oilseed rape buds were uniformly treated with dsRNA designed to target αCOP in B. aeneus. Our model control dsRNA (dsGFP) remained detectable on buds throughout the entire 3 d exposure period. When applied at 5 µg/µL, dsαCOP induced significant αCOP silencing 3 d after dietary exposure to buds treated with this dsαCOP concentration. We also observed a trend of increased αCOP silencing with increasing concentrations of dsαCOP at both 3 and 6 d. Furthermore, we observed a marginally significant and significant reduction in B. aeneus survival at 10 and 15 d, respectively. Our results suggest potential for developing a SIGS approach to B. aeneus management—though further experiments are needed to more fully understand this potential.

scholarly journals The effect of insecticide application by dropleg sprayers on pollen beetle parasitism in oilseed rape

BioControl ◽  
2021 ◽  
Author(s):  
Johannes Hausmann ◽  
Udo Heimbach ◽  
Michael Rostás ◽  
Meike Brandes
Keyword(s):  
Oilseed Rape ◽  
Insect Pests ◽  
Biological Control Agent ◽  
Control Agent ◽  
Total Catch ◽  
Pollen Beetle ◽  
Brassicogethes Aeneus ◽  
Larval Parasitoid ◽  
Parasitism Rates ◽  
The Impact

AbstractDropleg sprayers apply pesticides below the flower horizon of oilseed rape plants and thus reduce unwanted side effects on pollinating insects. Whether this technique benefits parasitoids of seed and pollen feeding insect pests has not been studied earlier. To answer this question, we first assessed the vertical distribution of pests and parasitoids using a portable aspirator. In addition, parasitism rates of pollen beetle, Brassicogethes aeneus Fabricius (Coleoptera: Nitidulidae), by the larval parasitoid Tersilochus heterocerus Thomson (Hymenoptera: Ichneumonidae) were compared in conventional and dropleg sprayed fields over four years (2016–2019), using the neonicotinoids thiacloprid and acetamiprid. Our results show that seed and pollen feeders were mainly found in the flowering canopy, while the predominant location of parasitoids was species-specific. Among pollen beetle parasitoids, Phradis interstitialis Thomson (Hymenoptera: Ichneumonidae) was more abundant below flowering canopy (63% of total catch), whereas T. heterocerus was mainly caught in the flowering canopy (84% of total catch). In the spraying experiments, average parasitism rates of pollen beetles by T. heterocerus ranged between 55 and 82% in the untreated controls. In the dropleg spray treatments, parasitism rates did not differ significantly from control levels, with the exception of thiacloprid application in 2019. In contrast, conventional spray applications resulted in a reduction of parasitism rates by up to 37% compared to the control for at least one of the insecticides in three out of four years. The impact of conventional application differed between years, which may be explained by the temporal coincidence between spray application and the immigration of parasitoids into the crop. We conclude that dropleg spraying exerts lower non-target effects on the main biological control agent of pollen beetle.

Evaluating the effect of seven plant essential oils on pollen beetle (Brassicogethes aeneus) survival and mobility

2020 ◽  
Vol 134 ◽  
pp. 105181
Author(s):  
Jonathan Willow ◽  
Silva Sulg ◽  
Eve Kaurilind ◽  
Ana Isabel Silva ◽  
Riina Kaasik ◽  
...  
Keyword(s):  
Essential Oils ◽  
Pollen Beetle ◽  
Plant Essential Oils ◽  
Brassicogethes Aeneus
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