scholarly journals Cytochrome P450s CYP380C6 and CYP380C9 in green peach aphid facilitate its adaptation to indole glucosinolate‐mediated plant defense

2020 ◽  
Vol 77 (1) ◽  
pp. 148-158
Author(s):  
Rui Ji ◽  
Jiaxin Lei ◽  
Ivy W Chen ◽  
Wen Sang ◽  
Shiying Yang ◽  
...  
Keyword(s):  
Plant Defense ◽  
Green Peach Aphid ◽  
Cytochrome P450s ◽  
Indole Glucosinolate

scholarly journals The Arabidopsis PAD4 Lipase-Like Domain Is Sufficient for Resistance to Green Peach Aphid

2020 ◽  
Vol 33 (2) ◽  
pp. 328-335 ◽  
Author(s):  
Joram A. Dongus ◽  
Deepak D. Bhandari ◽  
Monika Patel ◽  
Lani Archer ◽  
Lucas Dijkgraaf ◽  
...  
Keyword(s):  
Plant Defense ◽  
Insect Pests ◽  
Green Peach Aphid ◽  
Aphid Resistance ◽  
Molecular Evidence ◽  
Immune Functions ◽  
Transgenic Expression ◽  
Domain Specific ◽  
Pathogenic Microbes

Plants have evolved mechanisms to protect themselves against pathogenic microbes and insect pests. In Arabidopsis, the immune regulator PAD4 functions with its cognate partner EDS1 to limit pathogen growth. PAD4, independently of EDS1, reduces infestation by green peach aphid (GPA). How PAD4 regulates these defense outputs is unclear. By expressing the N-terminal PAD4 lipase-like domain (PAD4LLD) without its C-terminal EDS1-PAD4 (EP) domain, we interrogated PAD4 functions in plant defense. Here, we show that transgenic expression of PAD4LLD in Arabidopsis is sufficient for limiting GPA infestation but not for conferring basal and effector-triggered pathogen immunity. This suggests that the C-terminal PAD4 EP domain is necessary for EDS1-dependent immune functions but is dispensable for aphid resistance. Moreover, PAD4LLD is not sufficient to interact with EDS1, indicating the PAD4-EP domain is required for stable heterodimerization. These data provide molecular evidence that PAD4 has domain-specific functions.

scholarly journals Disruption of Ethylene Responses by Turnip mosaic virus Mediates Suppression of Plant Defense against the Green Peach Aphid Vector

2015 ◽  
Vol 169 (1) ◽  
pp. 209-218 ◽  
Author(s):  
Clare L. Casteel ◽  
Manori De Alwis ◽  
Aurélie Bak ◽  
Haili Dong ◽  
Steven A. Whitham ◽  
...  
Keyword(s):  
Plant Defense ◽  
Mosaic Virus ◽  
Green Peach Aphid ◽  
Turnip Mosaic Virus ◽  
Aphid Vector

Identification of indole glucosinolate breakdown products with antifeedant effects on Myzus persicae (green peach aphid)

2008 ◽  
Vol 54 (6) ◽  
pp. 1015-1026 ◽  
Author(s):  
Jae Hak Kim ◽  
Byong Won Lee ◽  
Frank C. Schroeder ◽  
Georg Jander
Keyword(s):  
Myzus Persicae ◽  
Green Peach Aphid ◽  
Indole Glucosinolate

scholarly journals Arabidopsis PAD4 lipase-like domain is a minimal functional unit in resistance to green peach aphid

2019 ◽  
Author(s):  
Joram A. Dongus ◽  
Deepak D. Bhandari ◽  
Monika Patel ◽  
Lani Archer ◽  
Lucas Dijkgraaf ◽  
...  
Keyword(s):  
Plant Defense ◽  
Functional Unit ◽  
Green Peach Aphid ◽  
Molecular Evidence ◽  
Immune Functions ◽  
Transgenic Expression ◽  
Beneficial Microbes ◽  
Domain Specific ◽  
Pathogenic Microbes

AbstractPlants have evolved mechanisms to attract beneficial microbes and insects while protecting themselves against pathogenic microbes and pests. In Arabidopsis, the immune regulator PAD4 functions with its cognate partner EDS1 to limit pathogen growth. PAD4, independently of EDS1, reduces infestation by Green Peach Aphid (GPA). How PAD4 regulates these defense outputs is unclear. By expressing the N-terminal PAD4-lipase-like domain (LLD) without its C-terminal ‘EDS1-PAD4’ (EP) domain, we interrogated PAD4 functions in plant defense. Here we show that transgenic expression of PAD4LLD in Arabidopsis is sufficient for limiting GPA infestation, but not for conferring basal and effector-triggered pathogen immunity. This suggests that the C-terminal PAD4-EP domain is necessary for EDS1-dependent immune functions. Moreover, PAD4LLD is not sufficient to interact with EDS1, indicating the PAD4-EP domain is required for heterodimerisation. These data provide molecular evidence that PAD4 has domain specific functions.

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