Discovery of a species‐specific novel antifungal compound against Fusarium graminearum through an integrated molecular modeling strategy

2020 ◽  
Vol 76 (12) ◽  
pp. 3990-3999
Author(s):  
Weitao Fu ◽  
Ningjie Wu ◽  
Di Ke ◽  
Yun Chen ◽  
Tianming Xu ◽  
...  
Keyword(s):  
Molecular Modeling ◽  
Fusarium Graminearum ◽  
Antifungal Compound ◽  
Species Specific ◽  
Modeling Strategy

scholarly journals Trichothecene Genotypes of Fusarium graminearum Populations Isolated from Winter Wheat Crops in Serbia

Toxins ◽  
2018 ◽  
Vol 10 (11) ◽  
pp. 460 ◽  
Author(s):  
Vesna Krnjaja ◽  
Slavica Stanković ◽  
Ana Obradović ◽  
Tanja Petrović ◽  
Violeta Mandić ◽  
...  
Keyword(s):  
Fusarium Graminearum ◽  
Gene Clusters ◽  
Sensu Stricto ◽  
Molecular Techniques ◽  
Weather Data ◽  
Morphological Observation ◽  
Specific Primer ◽  
Head Blight ◽  
As Species ◽  
Species Specific

Fusarium graminearum as the main causal agent of Fusarium head blight (FHB) and its ability to produce trichothecenes was investigated by molecular techniques. A total of 37 strains isolated from the wheat, harvested in Serbia in 2005, 2008 and 2015, and previously designated by morphological observation as F. graminearum, were used for trichothecene genotypes characterization. The strains were identified using the species-specific primer set FG16R/FG16F while genotypic characterization was done using specific TRI13 and TRI3 sequences of the trichothecene gene clusters. The PCR assays identified all strains as species of F. graminearum sensu stricto with the DON/15-ADON genotype. The quantification of the mycotoxin (DON) was performed using the biochemical assay. The high levels of DON (>20,000 µg kg−1) were recorded in all of the strains from 2005, four strains from 2008 and two strains from 2015. Weather data of the investigated seasons, showed that the optimal temperature, frequent rains and high relative humidity (RH) was very favourable for the development of F. graminearum, affecting the DON biosynthesis.

Quantification of Fusarium graminearum in infected wheat by species specific real-time PCR applying a TaqMan Probe

2004 ◽  
Vol 59 (1) ◽  
pp. 141-146 ◽  
Author(s):  
G.H. Reischer ◽  
M. Lemmens ◽  
A. Farnleitner ◽  
A. Adler ◽  
R.L. Mach
Keyword(s):  
Real Time ◽  
Fusarium Graminearum ◽  
Real Time Pcr ◽  
Taqman Probe ◽  
Species Specific

scholarly journals Fitness traits of deoxynivalenol and nivalenol-producing Fusarium graminearum species complex strains from wheat

2017 ◽  
Author(s):  
C.P. Nicolli ◽  
F.J. Machado ◽  
P. Spolti ◽  
E.M. Del Ponte
Keyword(s):  
Fusarium Graminearum ◽  
Mycelial Growth ◽  
Species Complex ◽  
Head Blight ◽  
Fitness Traits ◽  
Fusarium Graminearum Species Complex ◽  
Important Trait ◽  
South Of Brazil ◽  
Species Specific ◽  
Moderately Resistant

AbstractFusarium graminearum of the 15-acetyl(A)deoxynivalenol(D0N) chemotype is the main cause of Fusarium head blight (FHB) of wheat in south of Brazil. However, 3-ADON and nivalenol(NIV) chemotypes have been found in other members of the species complex causing FHB in wheat. To improve our understanding of the pathogen ecology, we assessed a range of fitness-related traits in a sample of 30 strains representatives of 15-ADON (F. graminearum), 3-ADON (F. cortaderiae and F. austroamericanum) and NIV (F. meridionale and F. cortaderiae). These included: perithecia formation on three cereal-based substrates, mycelial growth at two suboptimal temperatures, sporulation and germination, pathogenicity towards a susceptible and a moderately resistant cultivar and sensitivity to tebuconazole. The most important trait favoring F. graminearum was its 2x higher sexual fertility (> 40% PPI = perithecia production index) than the other species (< 30% PPI); PPI varied among substrates (maize > rice > wheat). In addition, sensitivity to tebuconazole appeared lower in F. graminearum which had the only strain with EC50 > 1 ppm. In the pathogenicity assays, the DON-producers were generally more aggressive (1.5 to 2x higher final severity) towards the two cultivars, with 3-ADON or 15-ADON leading to higher area under the severity curve than the NIV strains in the susceptible and moderately resistant cv., respectively. There was significant variation among strains of a same species with regards asexual fertility (mycelial growth, macroconidia production and germination), which suggest a strain-rather than a species-specific differences. These results contribute new knowledge to improve our understanding of the pathogen-related traits that may explain the dominance of certain members of the species complex in specific wheat agroecosystems.

scholarly journals First Report of the Nivalenol Chemotype of Fusarium graminearum Causing Head Blight of Wheat in the Grand Duchy of Luxembourg

Plant Disease ◽  
2009 ◽  
Vol 93 (11) ◽  
pp. 1217-1217 ◽  
Author(s):  
M. Pasquali ◽  
F. Giraud ◽  
C. Brochot ◽  
L. Hoffmann ◽  
T. Bohn
Keyword(s):  
Fusarium Graminearum ◽  
Aerial Mycelium ◽  
Triticum Aestivum L ◽  
Negative Control ◽  
Head Blight ◽  
First Report ◽  
Low Levels ◽  
Species Specific ◽  
Control Water ◽  
Positive Controls

Head blight caused by Fusarium graminearum is one of the major diseases of wheat (Triticum aestivum L.) in Luxembourg (2) and there is concern for mycotoxins in diseased grain. Isolates of F. graminearum have been assigned to chemotypes based on the particular toxins produced. Ten wheat fields representing different topoclimatological areas of Luxembourg were surveyed in 2007 and 2008 to determine the frequency and distribution of chemotypes. Partially blighted wheat heads were collected, and diseased grains were plated on Fusarium-selective agar (dichloran-chloramphenicol-peptone) for 12 days at 22 ± 2°C with a 12-h light period. Monoconidial isolates of F. graminearum (79 in 2007 and 85 in 2008) were obtained by conidia dilution on 2% water agar and needle selection under a microscope. F. graminearum isolates showed rapid growth on potato dextrose agar, dense aerial mycelium with red pigment deposits in the plate, macroconidia with five to six defined septa, and a basal cell with the typical foot shape. Microconidia were absent. To confirm species identification, a PCR reaction was carried out using the F. graminearum species-specific primers Fg16F (5′-CTCCGGATATGTTGCGTCAA-3′) and Fg16R (5′-GGTAGGTATCCGACATGGCAA-3′) according to Demeke et al. (1). Chemotype of each isolate was determined according to Ward et al. (4). In particular, PCR primer 12CON (5′ CATGAGCATGGTGATGTC-3′) coupled with primer 12NF (5′-TCTCCTCGTTGTATCTGG-3′) and primer 3CON (5′-TGGCAAAGACTGGTTCAC-3′) coupled with primer 3NA (5′-GTGCACAGAATATACGAGC-3′) identified the nivalenol chemotype, primer 12CON coupled with primer 12-15F (5′-TACAGCGGTCGCAACTTC-3′) and primer 3CON coupled with primer 3D15A (5′-ACTGACCCAAGCTGCCATC-3′) identified the 15-acetylated deoxynivalenol (DON) chemotype, while primer 12CON coupled with primer 12-3F (5′-CTTTGGCAAGCCCGTGCA-3′) and primer 3CON coupled with primer 3D3A (5′-CGCATTGGCTAACACATG-3′) identified 3-acetylated DON chemotype. Reactions were repeated two times and positive controls (provided by Kerry O'Donnell, NRRL collection, Peoria, IL) and a negative control (water) were used in each reaction. Frequency of the nivalenol chemotype was found to be 2.5% in 2007 and 1% in 2008. Interestingly, the nivalenol chemotype was absent in southern Luxembourg. According to this finding, nivalenol was likely to be present at low levels in grain from Reisdorf and Echternach in 2007 (central Luxembourg) and in 2008 from grain of Troisvierges (northern Luxembourg). The remaining isolates in both years belonged to the 15-acetylated DON chemotype and the 3-acetylated DON chemotype was not detected. Compared with a previous report from the Netherlands (3), the nivalenol chemotype in Luxembourg is less frequent and widespread. To our knowledge, this is the first report of the nivalenol chemotype of F. graminearum causing head blight on wheat in Luxembourg. References:(1) T. Demeke et al. Int. J. Food Microbiol. 103:271, 2005. (2) F. Giraud et al. Plant Dis. 92:1587, 2008. (3) C. Waalwijk et al. Eur. J. Plant Pathol. 109:743, 2003. (4) T. J. Ward et al. Fung. Genet. Biol. 45:473, 2008.

Identification and Genetic Division of Fusarium graminearum and Fusarium asiaticum by Species-Specific SCAR Markers

2013 ◽  
Vol 162 (2) ◽  
pp. 81-88 ◽  
Author(s):  
Xu Zhang ◽  
Hong-xiang Ma ◽  
Yong-jin Zhou ◽  
Jin-cheng Xing ◽  
Jian-hua Chen ◽  
...  
Keyword(s):  
Fusarium Graminearum ◽  
Scar Markers ◽  
Fusarium Asiaticum ◽  
Species Specific ◽  
Genetic Division

An in silico approach to discover the best molecular modeling strategy for designing novelCDK4 inhibitors

2019 ◽  
Vol 93 (4) ◽  
pp. 556-569 ◽  
Author(s):  
Divya Vasanthakumari ◽  
Pushpa Vadakkethil Lalithabhai ◽  
Manoj Kanthimathi Bahuleyan
Keyword(s):  
Molecular Modeling ◽  
In Silico ◽  
Modeling Strategy
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