Inheritance of Bacillus thuringiensis Cry2Ab2 protein resistance in Helicoverpa zea (Lepidoptera: Noctuidae)

2020 ◽  
Vol 76 (11) ◽  
pp. 3676-3684 ◽  
Author(s):  
Fei Yang ◽  
Graham P Head ◽  
Paula A Price ◽  
José C Santiago González ◽  
David L Kerns
Keyword(s):  
Bacillus Thuringiensis ◽  
Helicoverpa Zea ◽  
Protein Resistance ◽  
Lepidoptera Noctuidae

Field Evaluation of Transgenic Corn Containing a Bacillus thuringiensis Berliner Insecticidal Protein Gene Against Helicoverpa zea (Lepidoptera: Noctuidae)

1996 ◽  
Vol 31 (3) ◽  
pp. 340-346 ◽  
Author(s):  
Steven R. Sims ◽  
Jay C. Pershing ◽  
Barbara J. Reich
Keyword(s):  
Bacillus Thuringiensis ◽  
Helicoverpa Zea ◽  
Negative Relationship ◽  
Field Evaluation ◽  
Corn Earworm ◽  
Insecticidal Protein ◽  
Transgenic Corn ◽  
First Instar ◽  
Transgenic Lines ◽  
Lepidoptera Noctuidae

Twelve independently transformed lines of transgenic corn (Zea mays L.) expressing the CryIA(b) insecticidal protein from Bacillus thuringiensis var. kurstaki were field tested to evaluate their resistance to the corn earworm, Helicoverpa zea (Boddie). Silks of the primary (=top) ears of transgenic [CryIA(b) positive] and isoline control plants [no CryIA(b) protein] were artificially infested with first-instar H. zea larvae and the length of ear penetration was measured after 19 d. Eight of the 12 lines had significantly less ear damage than their respective isoline controls; 3 transgenic lines reduced H. zea feeding damage by > 75% and stunted surviving H. zea larvae. Concentration of the CryIA(b) protein (μg/g fresh weight) in silks of the transgenic lines, determined using ELISA, ranged from 0.0 to 1.28 μg/g. Within transgenic lines, there was a weak (P < 0.06) negative relationship between the concentration of CryIA(b) protein in fresh silks and the length of H. zea ear penetration.

Bollworm (Lepidoptera: Noctuidae) Development and Movement on Bacillus thuringiensis-treated Cotton Leaves

2001 ◽  
Vol 36 (1) ◽  
pp. 23-33 ◽  
Author(s):  
Muhammad Ashfaq ◽  
S. Y. Young ◽  
R. W. McNew
Keyword(s):  
Bacillus Thuringiensis ◽  
Helicoverpa Zea ◽  
Leaf Surface ◽  
Larval Mortality ◽  
Lethal Dose ◽  
Crop Protection ◽  
Pupal Weight ◽  
Larval Weight ◽  
Lepidoptera Noctuidae ◽  
Transfer Times

Larval mortality, survivorship and movement of the bollworm, Helicoverpa zea (Boddie), on and away from Bacillus thuringiensis-treated cotton leaves were investigated in the laboratory. Bacillus thuringiensis (Costar®, Novartis Crop Protection, Inc; Greensboro, NC) was applied to the upper surface of cotton leaves via a spray table in five concentrations, i.e., 0.0 (control), 0.14, 0.29, 0.58, and 1.15 kg/ha. Three-day-old H. zea larvae were released on treated leaves, kept at 30°C, and transferred to artificial diet after 12, 24, and 48 h. Significantly higher numbers of larvae were found on the lower than upper leaf surface after all three transfer times, but the numbers surviving in all treatments were similar to the control. Larval movement from the leaf to cup surface was significantly higher in B. thuringiensis-treated leaves than in the control. The mortality of larvae that were transferred from leaves to diet at 7 d after treatment was significantly higher in all treatments than in the control and highest at the highest rates (0.58 and 1.15 kg/ha). Data on survival of larvae at different locations suggest that for the first 24 h, the increase in the percentage of larvae on the inner cup surface in B. thuringiensis treatments was not due to larvae that had consumed a lethal dose, but an attempt to avoid the B. thuringiensis on the upper leaf surface. Highest larval weight was recorded in the control for all transfer times. The length of the larval period increased with the B. thuringiensis rate at the 12-h transfer. The pupal weight was reduced at the highest B. thuringiensis rates at the 48-h transfer.

Antagonism between Formulations of Different Bacillus thuringiensis Subspecies in Heliothis virescens and Helicoverpa zea (Lepidoptera: Noctuidae)

1998 ◽  
Vol 33 (2) ◽  
pp. 129-135 ◽  
Author(s):  
A. O. Ameen ◽  
J. R. Fuxa ◽  
A. R. Richter
Keyword(s):  
Bacillus Thuringiensis ◽  
Heliothis Virescens ◽  
Helicoverpa Zea ◽  
Insect Species ◽  
Median Lethal Concentrations ◽  
Lepidoptera Noctuidae

Interactions between formulations of the aizawai and kurstaki subspecies of Bacillus thuringiensis Berliner were evaluated by bioassay in Heliothis virescens (F.) and Helicoverpa zea (Boddie). In preliminary experiments, a formulation of subspecies aizawai, Xentari AS®, had significantly (P < 0.05) higher median lethal concentrations (LC50s) in both insect species than formulations based on subspecies kurstaki. Helicoverpa zea was significantly (P < 0.05) more susceptible than H. virescens to one formulation of subspecies kurstaki (Dipel ES®), but the two insects did not differ in susceptibility to Xentari AS® or to a second formulation of subspecies kurstaki (Dipel 6AF®). In H. virescens, Xentari AS® was additive with Dipel 6AF® and significantly (P< 0.05) antagonistic with Dipel ES® and with a third formulation of subspecies kurstaki, Dipel 48A®. In H. zea, Xentari AS® was significantly antagonistic with all three formulations of subspecies kurstaki. This suggests that certain toxin combinations from B. thuringiensis subspecies might not be effective for managing H. virescens and H. zea populations.

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