scholarly journals Compatibility between the endoparasitoid Hyposoter didymator and the entomopathogenic fungus Metarhizium brunneum : a laboratory simulation for the simultaneous use to control Spodoptera littoralis

2019 ◽  
Vol 76 (3) ◽  
pp. 1060-1070 ◽  
Author(s):  
Pedro Miranda‐Fuentes ◽  
Enrique Quesada‐Moraga ◽  
Hani K Aldebis ◽  
Meelad Yousef‐Naef
Keyword(s):  
Entomopathogenic Fungus ◽  
Spodoptera Littoralis ◽  
Laboratory Simulation ◽  
Metarhizium Brunneum ◽  
Simultaneous Use ◽  
Hyposoter Didymator

scholarly journals Telomere Length De Novo Assembly of all 7 Chromosomes and Mitogenome Sequencing of the Model Entomopathogenic Fungus, Metarhizium Brunneum, by Means of a Novel Assembly Pipeline

2020 ◽  
Author(s):  
Zack Saud ◽  
Alexandra M. Kortsinoglou ◽  
Vassili N. Kouvelis ◽  
Tariq M. Butt
Keyword(s):  
Entomopathogenic Fungus ◽  
De Novo ◽  
Gene Prediction ◽  
Fungal Species ◽  
Orthologous Protein ◽  
Metarhizium Brunneum ◽  
Sequencing Technologies ◽  
Protein Clusters ◽  
Assembly Pipeline ◽  
Generation Sequencing

Abstract More accurate and complete reference genomes have improved understanding of gene function, biology, and evolutionary mechanisms. Hybrid genome assembly approaches leverage benefits of both long, relatively error-prone reads from third-generation sequencing technologies and short, accurate reads from second-generation sequencing technologies, to produce more accurate and contiguous de novo genome assemblies in comparison to using either technology independently. In this study, we present a novel hybrid assembly pipeline that allowed for both mitogenome de novo assembly and telomere length de novo assembly of all 7 chromosomes of the model entomopathogenic fungus, Metarhizium brunneum. The improved assembly allowed for better ab initio gene prediction and a more BUSCO complete proteome set has been generated in comparison to the eight current NCBI reference Metarhizium spp. genomes. Remarkably, we note that including the mitogenome in ab initio gene prediction training improved overall gene prediction. The assembly was further validated by comparing contig assembly agreement across various assemblers, assessing the assembly performance of each tool. Genomic synteny and orthologous protein clusters were compared between Metarhizium brunneum and three other Hypocreales species with complete genomes, identifying core proteins, and listing orthologous protein clusters shared uniquely between the two entomopathogenic fungal species, so as to further facilitate the understanding of molecular mechanisms underpinning fungal-insect pathogenesis. The novel assembly pipeline may be used for other haploid fungal species, facilitating the need to produce high-quality reference fungal genomes, leading to better understanding of fungal genomic evolution, chromosome structuring and gene regulation.

scholarly journals Volatiles of the entomopathogenic fungus, Metarhizium brunneum, attract and kill plant parasitic nematodes

2021 ◽  
Vol 152 ◽  
pp. 104472
Author(s):  
Salim Khoja ◽  
Khalifa M. Eltayef ◽  
Ian Baxter ◽  
Arben Myrta ◽  
James C. Bull ◽  
...  
Keyword(s):  
Entomopathogenic Fungus ◽  
Parasitic Nematodes ◽  
Plant Parasitic Nematodes ◽  
Metarhizium Brunneum ◽  
Attract And Kill ◽  
Plant Parasitic

Redefining the dose of the entomopathogenic fungus Metarhizium brunneum (Ascomycota, Hypocreales) to increase Fe bioavailability and promote plant growth in calcareous and sandy soils

2017 ◽  
Vol 418 (1-2) ◽  
pp. 387-404 ◽  
Author(s):  
Silvia Raya–Díaz ◽  
Enrique Quesada–Moraga ◽  
Vidal Barrón ◽  
María Carmen del Campillo ◽  
Antonio Rafael Sánchez–Rodríguez
Keyword(s):  
Plant Growth ◽  
Entomopathogenic Fungus ◽  
Sandy Soils ◽  
Metarhizium Brunneum ◽  
Promote Plant Growth

Hydrophobin genes of the entomopathogenic fungus, Metarhizium brunneum, are differentially expressed and corresponding mutants are decreased in virulence

2012 ◽  
Vol 58 (2) ◽  
pp. 79-92 ◽  
Author(s):  
Ali Sevim ◽  
Bruno G. G. Donzelli ◽  
Dongliang Wu ◽  
Zihni Demirbag ◽  
Donna M. Gibson ◽  
...  
Keyword(s):  
Entomopathogenic Fungus ◽  
Differentially Expressed ◽  
Metarhizium Brunneum
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