In vitro nematicidal activity of natural and semisynthetic cadinenes from Heterotheca inuloides against the plant‐parasitic nematode Nacobbus aberrans (Tylenchida: Pratylenchidae)

2019 ◽  
Vol 75 (6) ◽  
pp. 1734-1742
Author(s):  
José Luis Rodríguez‐Chávez ◽  
Francisco Franco‐Navarro ◽  
Guillermo Delgado
Keyword(s):  
Parasitic Nematode ◽  
Plant Parasitic Nematode ◽  
Nematicidal Activity ◽  
Nacobbus Aberrans ◽  
Plant Parasitic

scholarly journals In vitro investigations on the biological control of Xiphinema index with Trichoderma species

2013 ◽  
Vol 50 (2) ◽  
pp. 132-137 ◽  
Author(s):  
Á. Daragó ◽  
M. Szabó ◽  
K. Hrács ◽  
A. Takács ◽  
P. Nagy
Keyword(s):  
Biological Control ◽  
Parasitic Nematode ◽  
Plant Parasitic Nematode ◽  
Trichoderma Spp ◽  
Trichoderma Species ◽  
Xiphinema Index ◽  
Promising Tool ◽  
Innate Ability ◽  
Plant Parasitic

AbstractThe application of Trichoderma spp. for the suppression of plant-parasitic nematode populations is a promising tool in biological control. Sixteen strains of six Trichoderma species (T. atroviride, T. harzianum, T. rossicum, T. tomentosum, T. virens and T. asperellum) were tested in vitro in order to identify the most appropriate strains to control the dagger nematode Xiphinema index. Mortality assays revealed that the strains of the widely investigated T. harzianum species have caused significant reduction of X. index populations, although T. harzianum strains were not the most efficient among all the tested fungi. Certain T. virens and T. atroviride strains and T. rossicum have triggered faster and higher mortality. Generally, our data indicate that Trichoderma species have innate ability to decrease X. index population. Furthermore, as we had difficulties with maintaining X. index in vitro, we successfully used a newly developed method to keep X. index specimens viable during the experiments.

Effects of exogenous amino acid applications on the plant-parasitic nematode Heterodera schachtii

Nematology ◽  
2018 ◽  
Vol 20 (8) ◽  
pp. 713-727 ◽  
Author(s):  
Roman Christopher Blümel ◽  
Daniel F. Fischer ◽  
Florian M.W. Grundler
Keyword(s):  
Amino Acid ◽  
Host Plant ◽  
Parasitic Nematode ◽  
Plant Parasitic Nematode ◽  
Heterodera Schachtii ◽  
Parasitic Nematodes ◽  
Direct Effects ◽  
Derivatives Of ◽  
Plant Parasitic

Summary Amino acid (AA) applications have been reported to affect plant-parasitic nematodes. Here, we analysed the effects of methionine (Met), lysine (Lys), threonine (Thr), isoleucine (Ile), homoserine (Hom) and tryptophan (Trp) on the sedentary plant-parasitic nematode, Heterodera schachtii, under in vitro conditions. No AA showed direct effects on the activity of infective second-stage juveniles (J2) of H. schachtii. Soaking J2 in Lys for 24 h increased the number of developing females and reduced the number of males. Thr treatments reduced the total number of nematodes developing in the host plant. The strongest effects were observed when AA were added to the nutrient medium in a monoxenic Arabidopsis thaliana culture. Ile, Met or Thr clearly reduced the number of female nematodes developing in the host plant. These AA are direct metabolic derivatives of Hom. Direct effects on pre-infective J2 can be differentiated from effects that may involve the host plant.

scholarly journals The role of Caenorhabditis elegans sex-determination homologs, Mi-sdc-1 and Mi-tra-1 in Meloidogyne incognita

2021 ◽  
Author(s):  
Anil Baniya ◽  
Soumi Joseph ◽  
Larry Duncan ◽  
William Crow ◽  
Tesfamariam Mengistu
Keyword(s):  
Caenorhabditis Elegans ◽  
Sex Determination ◽  
Meloidogyne Incognita ◽  
Egg Production ◽  
Target Genes ◽  
Parasitic Nematode ◽  
Plant Parasitic Nematode ◽  
Root Knot Nematode ◽  
Model Free ◽  
Plant Parasitic

AbstractSex determination is a key developmental event in all organisms. The pathway that regulates sexual fate has been well characterized at the molecular level in the model free-living nematode Caenorhabditis elegans. This study aims to gain a preliminary understanding of sex-determining pathways in a plant-parasitic nematode Meloidogyne incognita, and the extent to which the roles of the sex determination genes are conserved in a hermaphrodite species, C. elegans, and plant-parasitic nematode species, M. incognita. In this study, we targeted two sex-determining orthologues, sdc-1 and tra-1 from M. incognita using RNA interference (RNAi). RNAi was performed by soaking second-stage juveniles of M. incognita in a solution containing dsRNA of either Mi-tra-1or Mi-sdc-1 or both. To determine the effect of RNAi of the target genes, the juveniles treated with the dsRNA were inoculated onto a susceptible cultivar of cowpea grown in a nutrient pouch at 28 °C for 5 weeks. The development of the nematodes was analyzed at different time points during the growth period and compared to untreated controls. Our results showed that neither Mi-sdc-1 nor Mi-tra-1 have a significant role in regulating sexual fate in M. incognita. However, the silencing of Mi-sdc-1 significantly delayed maturity to adult females but did not affect egg production in mature females. In contrast, the downregulation of Mi-tra-1 transcript resulted in a significant reduction in egg production in both single and combinatorial RNAi-treated nematodes. Our results indicate that M. incognita may have adopted a divergent function for Mi-sdc-1 and Mi-tra-1distinct from Caenorhabditis spp. However, Mi-tra-1 might have an essential role in female fecundity in M. incognita and is a promising dsRNA target for root-knot nematode (RKN) management using host-delivered RNAi.

Sign in / Sign up

Export Citation Format

Share Document