Antiviral activity of titanium dioxide nanostructures as a control strategy for broad bean strain virus in faba bean

2018 ◽  
Vol 75 (3) ◽  
pp. 828-834 ◽  
Author(s):  
Mohsen M Elsharkawy ◽  
Aly Derbalah
1976 ◽  
Vol 56 (3) ◽  
pp. 451-453 ◽  
Author(s):  
B. D. FRAZER ◽  
D. RAWORTH ◽  
T. GOSSARD

Eleven cultivars of faba beans and one of broad bean (Vicia faba L.) were bioassayed for resistance to pea aphids (Acyrthosiphon pisum (Harris)) by determining the fecundity, survival and developmental rate of the aphid on each cultivar. None of the cultivars tested, including the three licensed for production in Canada, possess any significant resistance, although they differ in susceptibility.


Plant Disease ◽  
2010 ◽  
Vol 94 (8) ◽  
pp. 1068-1068 ◽  
Author(s):  
S. G. Kumari ◽  
A. Najar ◽  
N. Attar ◽  
M. H. Loh ◽  
H.-J. Vetten

Chickpea plants with severe yellowing and tip wilting were observed in the Cap-Bon Region of Tunisia in 2006. The viral-like symptoms resulted in yield loss of approximately 25% in some fields. A total of 110 symptomatic chickpea plants was collected from nine chickpea fields and tested at the Virology Laboratory of ICARDA, Syria for eight legume viruses using tissue-blot immunoassay (TBIA) (3). Polyclonal antisera produced at the ICARDA Virology Laboratory were used to test for Chickpea chlorotic dwarf virus (genus Mastrevirus, family Geminiviridae), Broad bean stain virus (genus Comovirus, family Secoviridae), Broad bean mottle virus (genus Bromovirus, family Bromoviridae), and Bean yellow mosaic virus and Pea seed borne mosaic virus (genus Potyvirus, family Potyviridae). Antiserum to Beet mosaic virus (BtMV; genus Potyvirus, family Potyviridae) (AS-0143) was provided by the German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig, Germany). In addition, three monoclonal antibodies (MAb) were used to detect Faba bean necrotic yellows virus (FBNYV; genus Nanovirus, family Nanoviridae) (MAb 3-2E9) (1), potyviruses (PVAS-769 [MAb PTY 3 Potyvirus Group] American Type Culture Collection, Manassas, VA), and luteoviruses (MAb B-2-5G4) (2). Twenty-two of the plants tested positive with MAb PTY 3 and BtMV antisera, 56 samples reacted with MAb B-2-5G4, and eight plants with the FBNYV MAb, whereas 24 plants tested negative with all antisera. Because reactions with the BtMV antiserum were unexpected, detection of BtMV was confirmed by reverse transcription-(RT)-PCR assays using BtMV-specific primers (LN26 and LN27) (4), which produced an amplicon of expected size (1,050 bp) from all plants that reacted with BtMV antiserum but not from plants that were serologically negative. Leaf tissue from a BtMV-infected plant was ground in 0.01 M potassium phosphate buffer, pH 7.2 (1:20, wt/vol), mixed with 0.5% celite, and used for mechanical inoculation of chickpea seedlings (cv. Beja 4). In addition, adults of three legume aphid species (Aphis craccivora, A. fabae, and Acyrthosiphon pisum) were starved for 1 h before feeding on BtMV-infected chickpea leaves for an acquisition access period of 5 min. Fifteen aphids of each species were placed on each chickpea plant, allowed to feed for 24 h, and then sprayed with an insecticide. Tip wilting symptoms appeared on plants 15 to 20 days after mechanical and aphid inoculations but not on plants used as negative control treatments (inoculated mechanically with healthy leaf tissue or with aphids that had fed on noninfected chickpea plants). Use of BtMV antiserum for TBIA analysis of inoculated plants revealed systemic BtMV infections in 35 of 92 plants inoculated mechanically and 15 of 75 plants inoculated with viruliferous A. fabae only. To our knowledge, this is the first record of BtMV infecting chickpea in Tunisia. References: (1) A. Franz et al. Ann. Appl. Biol. 128:255, 1996. (2) L. Katul. Characterization by serology and molecular biology of bean leaf roll virus and faba bean necrotic yellows virus. Ph.D. thesis. University of Gottingen, Germany, 1992. (3) K. M. Makkouk and A. Comeau. Eur. J. Plant Pathol. 100:71, 1994. (4) L. G. Nemchinov et al. Arch. Virol. 149:1201, 2004.


Plant Disease ◽  
2000 ◽  
Vol 84 (9) ◽  
pp. 1046-1046 ◽  
Author(s):  
Asma Najar ◽  
Khaled M. Makkouk ◽  
Safaa G. Kumari

A survey was conducted in April 2000 to identify viruses infecting faba bean (Vicia faba L.) in seven regions (Beja, Bizerte, Cap-bon, Jendouba, Kairouan, Siliana, and Zaghouan) of Tunisia. Around 4,000 random samples were collected from 34 fields (100 to 200 samples per field). Samples were tested by tissue-blot immunoassay procedure (2) at the Virology Laboratory of INRAT for the presence of 14 different viruses. All antisera used were specific, including those for Faba bean necrotic yellows virus (FBNYV) (1) and Beet western yellows virus (BWYV) (ATCC-PVAS-647). Broad bean mottle, a beetle-transmitted and seedborne virus, was the most abundant with an average incidence of 2.3%. The highest disease incidence occurred with BWYV, which was in 20 and FBNYV in 4 of 98 random samples from a field in the Siliana region; and with BWYV in 7 and BBMV in 21 of 120 samples from a field in the Jendouba region. Other viruses such as broad bean stain, cucumber mosaic, and bean leaf roll were detected rarely (<1%). This is the first record of FBNYV and BWYV infecting faba bean in Tunisia. BWYV was detected in 1.2% of the samples tested collected from 20 fields comprising all seven regions. FBNYV was detected in 0.9% of the samples tested collected from 13 fields comprising all except the Capbon region. References: (1) A. Franz et al. Ann. Appl. Biol. 128:255, 1996. (2) K. M. Makkouk and A. Comeau. Eur. J. Plant Pathol. 100:71, 1994.


Pulses ◽  
2020 ◽  
pp. 27-54
Author(s):  
Lamia L’Hocine ◽  
Delphine Martineau-Côté ◽  
Allaoua Achouri ◽  
Janitha P. D. Wanasundara ◽  
Gayani W. Loku Hetti Arachchige
Keyword(s):  

2016 ◽  
Vol 4 (2) ◽  
pp. 107 ◽  
Author(s):  
Fouad Meradsi ◽  
Malik Laamari

The Black bean aphid Aphis fabae Scopoli (Homoptera: Aphididae) is a serious pest of faba bean, Vicia faba L. (Fabaceae) in Algeria. The use of resistant cultivars may reduce the impact of this pest on broad bean production and at the same time reduce environmental risks and control costs. The level of resistance of fourteen cultivars of broad bean, Vicia faba was screened in the field against the main aphid pest of faba bean, the Black bean aphid, Aphis fabae Scopoli during January to April 2011 in Biskra region (in the east of Algeria). Among the cultivars tested, six cultivars (1, 9, 12, 13, 14 and 15) were the most resistant in the three counts, while three cultivars (4, 6 and 11) were showed a high susceptibility against this aphid at least once. The remainder of cultivars (3, 5, 7, 8 and 10) revealed an unsettled level of resistance. The relationship between the level of resistance and morphological characters of cultivars indicated that the resistant cultivars have a longer leaflet than the high susceptible cultivars. In the other hand, the statistical analysis showed that no significant differences on the plant height and the leaflet width in the tow category of cultivars (resistant and high susceptible).   


Author(s):  
K. Subramanya Sastry ◽  
Bikash Mandal ◽  
John Hammond ◽  
S. W. Scott ◽  
R. W. Briddon
Keyword(s):  

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