Analysis of RPA190 revealed multiple positively selected mutations associated with metalaxyl resistance in Phytophthora infestans

2018 ◽  
Vol 74 (8) ◽  
pp. 1916-1924 ◽  
Author(s):  
Fengping Chen ◽  
Qian Zhou ◽  
Jing Xi ◽  
Dong-liang Li ◽  
Guido Schnabel ◽  
...  
Keyword(s):  
Phytophthora Infestans ◽  
Metalaxyl Resistance

The structure of mating type, virulence, metalaxyl resistance of Phytophthora infestans in a long-term phenotypic study in distinct location in Eastern Estonia

2012 ◽  
Vol 119 (2) ◽  
pp. 45-52 ◽  
Author(s):  
Eve Runno-Paurson ◽  
Asko Hannukkala ◽  
Stanislav Trdan ◽  
Ingrid Williams ◽  
Mati Koppel ◽  
...  
Keyword(s):  
Phytophthora Infestans ◽  
Mating Type ◽  
Metalaxyl Resistance

Metalaxyl resistance, mating type and pathogenicity of Phytophthora infestans in Uganda

2001 ◽  
Vol 20 (5) ◽  
pp. 379-388 ◽  
Author(s):  
J. Mukalazi ◽  
E. Adipala ◽  
T. Sengooba ◽  
J.J. Hakiza ◽  
M. Olanya ◽  
...  
Keyword(s):  
Phytophthora Infestans ◽  
Mating Type ◽  
Metalaxyl Resistance

scholarly journals Characterization of Isolates of Phytophthora infestans from Southern and Southeastern Brazil from 1998 to 2000

Plant Disease ◽  
2003 ◽  
Vol 87 (8) ◽  
pp. 896-900 ◽  
Author(s):  
Ailton Reis ◽  
Christine D. Smart ◽  
William E. Fry ◽  
Luiz A. Maffia ◽  
Eduardo S. G. Mizubuti
Keyword(s):  
Restriction Fragment Length Polymorphism ◽  
Phytophthora Infestans ◽  
Mating Type ◽  
Fragment Length Polymorphism ◽  
Southeastern Brazil ◽  
Mating Types ◽  
Clonal Lineage ◽  
Metalaxyl Resistance ◽  
Restriction Fragment Length

The population of Phytophthora infestans in Brazil was first characterized 12 years ago. In this research, isolates of P. infestans from potato (n = 184) and tomato (n = 267) collected in southern and southeastern Brazil were characterized to provide more detailed analysis of the current structure of the population. All 451 isolates were analyzed for mating type, and subsets of the isolates were analyzed for allozymes, restriction fragment length polymorphism fingerprint, mtDNA haplotypes, and metalaxyl resistance. Tomato isolates were all of A1 mating type, mtDNA Ib, and US-1 genotype or some variant within this clonal lineage. Of the potato isolates, 82% were A2 mating type, mtDNA IIa, BR-1 genotype, which is a new lineage of P. infestans. All A2 isolates were found on potato, whereas 91% of the A1 isolates were from tomato. A1 and A2 isolates were never found in the same field. The frequency of resistance to metalaxyl was higher in isolates from tomato (55%) than in isolates from potato (38%). After more than a decade of coexistence of isolates of the A1 and A2 mating types, the population was highly clonal, dominated by the BR-1 and US-1 clonal lineages.

scholarly journals Metalaxyl Resistance in Phytophthora infestans: Assessing Role of RPA190 Gene and Diversity Within Clonal Lineages

2015 ◽  
Vol 105 (12) ◽  
pp. 1594-1600 ◽  
Author(s):  
Michael E. H. Matson ◽  
Ian M. Small ◽  
William E. Fry ◽  
Howard S. Judelson
Keyword(s):  
Phytophthora Infestans ◽  
Nucleotide Polymorphism ◽  
High Resolution Melt ◽  
Gene Encoding ◽  
Single Nucleotide ◽  
Metalaxyl Resistance ◽  
The Us ◽  
Polymerase I ◽  
Late Blight Pathogen

Prior work has shown that the inheritance of resistance to metalaxyl, an oomycete-specific fungicide, is complex and may involve multiple genes. Recent research indicated that a single nucleotide polymorphism (SNP) in the gene encoding RPA190, the largest subunit of RNA polymerase I, confers resistance to metalaxyl (or mefenoxam) in some isolates of the potato late blight pathogen Phytophthora infestans. Using both DNA sequencing and high resolution melt assays for distinguishing RPA190 alleles, we show here that the SNP is absent from certain resistant isolates of P. infestans from North America, Europe, and Mexico. The SNP is present in some members of the US-23 and US-24 clonal lineages, but these tend to be fairly sensitive to the fungicide based on artificial media and field test data. Diversity in the level of sensitivity, RPA190 genotype, and RPA190 copy number was observed in these lineages but were uncorrelated. Controlled laboratory crosses demonstrated that RPA190 did not cosegregate with metalaxyl resistance from a Mexican and British isolate. We conclude that while metalaxyl may be used to control many contemporary strains of P. infestans, an assay based on RPA190 will not be sufficient to diagnose the sensitivity levels of isolates.

scholarly journals The structure of mating type, metalaxyl resistance and virulence of Phytophthora infestans isolates collected from Latvia

2015 ◽  
Vol 102 (3) ◽  
pp. 335-342 ◽  
Author(s):  
Alice Aav ◽  
Ilze Skrabule ◽  
Gunita Bimšteine ◽  
Tanel Kaart ◽  
Ingrid H. Williams ◽  
...  
Keyword(s):  
Phytophthora Infestans ◽  
Mating Type ◽  
Metalaxyl Resistance

Mating type, Metalaxyl Resistance and Aggressiveness of Phytophthora infestans (Mont.) de Bary in Morocco

2002 ◽  
Vol 150 (4-5) ◽  
pp. 289-291 ◽  
Author(s):  
A. HAMMI ◽  
Y. MSATEF ◽  
A. BENNANI ◽  
A. E. L. ISMAILI ◽  
M. N. SERRHINI
Keyword(s):  
Phytophthora Infestans ◽  
Mating Type ◽  
Metalaxyl Resistance
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