Seven-year monitoring of pyrethroid resistance in the pollen beetle (Brassicogethes aeneus F.) during implementation of insect resistance management

2017 ◽  
Vol 74 (1) ◽  
pp. 200-209 ◽  
Author(s):  
Jitka Stará ◽  
František Kocourek
Keyword(s):  
Insect Resistance ◽  
Pyrethroid Resistance ◽  
Resistance Management ◽  
Pollen Beetle ◽  
Insect Resistance Management ◽  
Brassicogethes Aeneus

scholarly journals ‘What I cannot create, I do not understand’: functionally validated synergism of metabolic and target site insecticide resistance

2020 ◽  
Vol 287 (1927) ◽  
pp. 20200838 ◽  
Author(s):  
George-Rafael Samantsidis ◽  
Rafaela Panteleri ◽  
Shane Denecke ◽  
Stella Kounadi ◽  
Iason Christou ◽  
...  
Keyword(s):  
Insecticide Resistance ◽  
Crop Production ◽  
Pyrethroid Resistance ◽  
Malaria Incidence ◽  
Resistance Management ◽  
Underlying Mechanism ◽  
Target Site ◽  
Pollen Beetle ◽  
Brassicogethes Aeneus ◽  
Target Site Resistance

The putative synergistic action of target-site mutations and enhanced detoxification in pyrethroid resistance in insects has been hypothesized as a major evolutionary mechanism responsible for dramatic consequences in malaria incidence and crop production. Combining genetic transformation and CRISPR/Cas9 genome modification, we generated transgenic Drosophila lines expressing pyrethroid metabolizing P450 enzymes in a genetic background along with engineered mutations in the voltage-gated sodium channel ( para ) known to confer target-site resistance. Genotypes expressing the yellow fever mosquito Aedes aegypti Cyp9J28 while also bearing the para V1016G mutation displayed substantially greater resistance ratio (RR) against deltamethrin than the product of each individual mechanism (RR combined : 19.85 > RR Cyp9J28 : 1.77 × RR V1016G : 3.00). Genotypes expressing Brassicogethes aeneus pollen beetle Cyp6BQ23 and also bearing the para L1014F ( kdr ) mutation, displayed an almost multiplicative RR (RR combined : 75.19 ≥ RR Cyp6BQ23 : 5.74 × RR L1014F : 12.74). Reduced pyrethroid affinity at the target site, delaying saturation while simultaneously extending the duration of P450-driven detoxification, is proposed as a possible underlying mechanism. Combinations of target site and P450 resistance loci might be unfavourable in field populations in the absence of insecticide selection, as they exert some fitness disadvantage in development time and fecundity. These are major considerations from the insecticide resistance management viewpoint in both public health and agriculture.

scholarly journals Potential shortfall of pyramided transgenic cotton for insect resistance management

2013 ◽  
Vol 110 (15) ◽  
pp. 5806-5811 ◽  
Author(s):  
T. Brevault ◽  
S. Heuberger ◽  
M. Zhang ◽  
C. Ellers-Kirk ◽  
X. Ni ◽  
...  
Keyword(s):  
Insect Resistance ◽  
Resistance Management ◽  
Transgenic Cotton ◽  
Insect Resistance Management
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