MicroRNA and dsRNA targeting chitin synthase A reveal a great potential for pest management of the hemipteran insect Nilaparvata lugens

2017 ◽  
Vol 73 (7) ◽  
pp. 1529-1537 ◽  
Author(s):  
Tengchao Li ◽  
Jie Chen ◽  
Xiaobin Fan ◽  
Weiwen Chen ◽  
Wenqing Zhang
Keyword(s):  
Pest Management ◽  
Nilaparvata Lugens ◽  
Chitin Synthase ◽  
Hemipteran Insect

scholarly journals Identification of a female determinant gene for the sexual determination of a hemipteran insect, the brown planthopper

2019 ◽  
Author(s):  
Ji-Chong Zhuo ◽  
Hou-Hong Zhang ◽  
Yu-Cheng Xie ◽  
Han-Jing Li ◽  
Qing-Ling Hu ◽  
...  
Keyword(s):  
Sex Determination ◽  
Brown Planthopper ◽  
Nilaparvata Lugens ◽  
Female Development ◽  
Upstream Gene ◽  
Sexual Determination ◽  
Specific Alternative ◽  
Hemipteran Insect ◽  
Female Determinant

AbstractThe sex determination mechanism for hemipteran species remains poorly understood. During the sex determination of the brown planthopper (BPH), Nilaparvata lugens, one species of Hemiptera, the functions of doublesex (Nldsx) and NlTra-2 (NlTra-2) genes were identified in our previous studies. Here, we identify an upstream gene for Nldsx in the sex determination cascade, NlFmd, which acts as female determinant gene for N. lugens. The sex-specific transcript of NlFmd (NlFmd-F) encodes an arginine/serine-, and proline-rich protein that is essential for female development. The knockdown of NlFmd resulted in the development of pseudomales, with sex-specific alternative Nldsx processing, and maternal RNA interference (RNAi) against NlFmd generates male-only progeny. Moreover, homologous genes for NlFmd have also been identified in two rice planthopper species, the white-backed planthopper (WBPH, Sogotalla furcifera) and the small brown planthopper (SBPH, Laodelphax striatellus), and these genes appear to be involved in the sex determination cascades for these species. Our data suggest that the sex determination cascade in Delphacidae is conserved.

Characterization and comparative profiling of the small RNA transcriptomes in the Hemipteran insect Nilaparvata lugens

Gene ◽  
2016 ◽  
Vol 595 (1) ◽  
pp. 83-91 ◽  
Author(s):  
Wenjun Zha ◽  
Lei Zhou ◽  
Sanhe Li ◽  
Kai Liu ◽  
Guocai Yang ◽  
...  
Keyword(s):  
Small Rna ◽  
Nilaparvata Lugens ◽  
Hemipteran Insect

scholarly journals Populasi Hama Wereng Batang Coklat (Nilaparvata lugens Stal.), Keragaman Musuh Alami Predator Serta Parasitoidnya Pada Lahan Sawah Di Dataran Rendah Kabupaten Indramayu

Agrologia ◽  
2017 ◽  
Vol 6 (1) ◽  
Author(s):  
Martua Suhunan Sianipar ◽  
Andang Purnama ◽  
Entun Santosa ◽  
R.C. Hidayat Soesilohadi ◽  
Wahyu Daradjat Natawigena ◽  
...  
Keyword(s):  
Integrated Pest Management ◽  
Pest Management ◽  
Diversity Index ◽  
Population Decline ◽  
Nilaparvata Lugens ◽  
Uniform Field ◽  
Wiener Diversity Index ◽  
Rice Pest ◽  
Nilaparvata Lugens Stål ◽  
Brown Plant Hopper

Population of brown plant hopper (Nilaparvata lugens Stal) is one of the major rice pest that is threatening rice production in Indramayu. Integrated pest management is needed to control the population of brown plant hopper (BPH). To construct effective strategy of Integrated Pest Management, a survey to obtain information about the population of BPH and the diversity of its natural enemies predator and parasitoid is required. The result of the survey showed that fluctuation of BPH population was evidence. BPH occurs from the 1st week of observation and the population was rising until the 5th week of observation and hence the population declined until the 12th week of observation. This population decline was caused by BPH’s live cycle followed the growth phase of rice plant. Correlation between climate factors and fluctuation of BPH population was not significant. Based on Shanon-Wiener diversity index analysis, the diversity of natural predators of BPH was medium with the value of H’ of 1,83 due to  uniform field condition

Characterization of putative soluble and membrane-bound trehalases in a hemipteran insect, Nilaparvata lugens

2009 ◽  
Vol 55 (11) ◽  
pp. 997-1002 ◽  
Author(s):  
Jianhua Gu ◽  
Ying Shao ◽  
Chengwei Zhang ◽  
Zewen Liu ◽  
Yongjun Zhang
Keyword(s):  
Nilaparvata Lugens ◽  
Membrane Bound ◽  
Hemipteran Insect

scholarly journals An MD-2-related lipid-recognition protein is required for insect reproduction and integument development

Open Biology ◽  
2021 ◽  
Vol 11 (12) ◽  
Author(s):  
Wei Wang ◽  
Ya Ma ◽  
Rui-Rui Yang ◽  
Xu Cheng ◽  
Hai-Jian Huang ◽  
...  
Keyword(s):  
Fat Body ◽  
Brown Planthopper ◽  
Nilaparvata Lugens ◽  
Egg Laying ◽  
Female Reproductive Success ◽  
Adult Transition ◽  
Recognition Protein ◽  
Hemipteran Insect ◽  
Insect Reproduction ◽  
Myeloid Differentiation Factor

The myeloid differentiation factor 2 (MD-2)-related lipid-recognition protein is involved in immune responses through recognizing bacteria lipopolysaccharide in mammals, arthropods and plants. However, the physiological roles of MD-2 in other biological processes are largely unknown. Here, we identified three homologue MD-2 genes ( NlML1 , NlML2 and NlML3 ) by searching the genome and transcriptome databases of the brown planthopper Nilaparvata lugens , a hemipteran insect species. Temporospatial analysis showed that the NlML1 gene was highly expressed in the fat body but much less so in the other tissues, while the NlML2 and NlML3 genes were highly expressed in the testis or digestive tract. RNA interference-mediated depletion of the NlML1 gene significantly downregulated the transcription of numerous integument protein genes. The NlML1 knockdown led to moulting failure and mortality at the nymph–adult transition phase, impaired egg laying and hatching, and reduced 20-hydroxyecdysone (20E) production in the nymphs. 20E could rescue the deficient moulting phenotypes derived from ds NlML1 RNAi. These novel findings indicate that NlML1 is required for nymphal moulting and female reproductive success as it plays an important role in regulating 20E synthesis, lipid and chitin metabolisms in N. lugens , thus contributing to our understanding of developmental and reproductive mechanisms in insects.

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