An entomopathogenic bacterium strain,Bacillus thuringiensis, as a biological control agent against the red palm weevil,Rhynchophorus ferrugineus(Coleoptera: Curculionidae)

2017 ◽  
Vol 73 (7) ◽  
pp. 1494-1502 ◽  
Author(s):  
Yu-chen Pu ◽  
Tian-ling Ma ◽  
You-ming Hou ◽  
Ming Sun
Keyword(s):  
Biological Control ◽  
Bacillus Thuringiensis ◽  
Biological Control Agent ◽  
Control Agent ◽  
Rhynchophorus Ferrugineus ◽  
Red Palm Weevil ◽  
Palm Weevil ◽  
Bacterium Strain

Pathogenicity of an Indigenous Strain of the Entomopathogenic Fungus Metarhizium anisopliae (Hypocreales: Clavicipitaceae) (MET-GRA4 Strain) as a Potential Biological Control Agent Against the Red Palm Weevil (Coleoptera: Dryophthoridae)

2019 ◽  
Author(s):  
I Ishak ◽  
L C Ng ◽  
M Haris-Hussain ◽  
J Jalinas ◽  
A B Idris ◽  
...  
Keyword(s):  
Biological Control ◽  
Dna Sequences ◽  
Metarhizium Anisopliae ◽  
Insect Pests ◽  
Biological Control Agent ◽  
Control Agent ◽  
Red Palm Weevil ◽  
Palm Weevil ◽  
Lethal Time

Abstract Metarhizium anisopliae Metchnikoff (Hypocreales: Clavicipitaceae) is a fungal pathogen that causes disease in various insect pests, and it can be exploited and developed as a biological control agent to combat the red palm weevil, Rhynchophorus ferrugineus Olivier (Coleoptera: Dryophthoridae). The study on indigenous isolates is crucial especially for development of bioinsecticides in the future. The M. anisopliae strain called MET-GRA4 was tested for pathogenicity against adult red palm weevil and treated in vitro with different spore viabilities. The isolates exhibited pathogenicity with 100% mortality 21 d postinfection. The median lethal time (LT50) for 85% viable spores was 8.6 d, while 39% viable spores had an LT50 value of 21.37 d, with 92 and 16.6% mycosis, respectively. The species MET-GRA4 strain was molecularly characterized using ITS1 and ITS4 from pure culture (Isolate A), mass-produced spores (Isolate B), and infected red palm weevil cadavers (Isolate C). The DNA sequences obtained matched M. anisopliae sequences, with 99% similarity. This new isolate of M. anisopliae has potential as a targeted bioinsecticide for management of red palm weevil.

Evaluation of a formulation of Bacillus thuringiensis against waxmoths in stored honeycombs

1991 ◽  
Vol 31 (5) ◽  
pp. 709 ◽  
Author(s):  
SC McKillup ◽  
DG Brown
Keyword(s):  
Biological Control ◽  
Apis Mellifera ◽  
Bacillus Thuringiensis ◽  
Field Experiment ◽  
Biological Control Agent ◽  
Control Agent ◽  
Control Methods ◽  
Commercial Formulation ◽  
Significant Damage ◽  
The Cost

Waxmoths cause significant damage to stored honeycombs of the Western honeybee Apis mellifera in Australia. A field experiment was designed to evaluate the effectiveness of a commercial formulation (Certan) of the biological control agent Bacillus thuringiensis in preventing this damage.Treatment applied at the manufacturer's recommended rate of 855 units per cm2 of honeycomb almost completely prevented damage, while untreated combs showed an average of 76% damage. The cost and practicality of applying the formulation of B. thuringiensis are discussed, together with the recommendation that new control methods for waxmoths should be researched.

scholarly journals Evaluating the efficacy of Trichoderma harzianum and Bacillus thuringiensis on induce the plant growth and resistance of local variety patchouli under covered and uncovered seedlings methods

2022 ◽  
Vol 951 (1) ◽  
pp. 012106
Author(s):  
R Sriwati ◽  
T Chamzurni ◽  
F Razi ◽  
Syaifullah ◽  
Yunita ◽  
...  
Keyword(s):  
Biological Control ◽  
Bacillus Thuringiensis ◽  
Plant Growth ◽  
Biological Control Agent ◽  
Dose Range ◽  
Morphological Characters ◽  
Control Agent ◽  
Plant Diseases ◽  
Biological Control Agents ◽  
Pests And Diseases

Abstract To increase plant resistance from an early age, it is necessary to introduce biological control agents from groups of fungi and bacteria. This study aims to determine the effect of Trichoderma harziaunum and Bacillus thuringiensis Aceh isolates in increasing the superiority of Aceh patchouli plants that are resistant to pests and plant diseases. The study used non-factorial RAL method with cover and uncovered seedling methods. Both series were treated with the same biological control agent, the control without any treatment, the treatment of T. harzianum and B. thuringiensis while the observations were made when the seedling covered was opened. Observations included plant morphological characters, plant growth development and peroxidase enzymes. The results showed that morphologically the original patchouli growing in Lhoong district had similar morphological characters to the Lhokseumawe variety. The application of biological control agents of the T. harzianum and B. thuringiensis groups was more effective in increasing plant growth in the closed seedling treatment compared to the uncovered seedling. T. harzianum gave the best effect at a dose range of 1-1.5 while B. thuringensis showed a better effect at a concentration of 10-15 ml. Both treatments increased the growth of patchouli seedlings as indicated by the better plant height and number of shoots. Furthermore, higher peroxidation enzymes were found in the closed seedling treatment with 1.5 g T harzianum and 15 ml B. thuringiensis. The high peroxidase enzyme as an indicator of the more resistant plants have been induced to pests and diseases. From the screen house experiment, T. harzianum and B. thuringiensis were more efficient in inducing plant growth and disease resistance of local varieties of patchouli using the closed seedling method.

Sign in / Sign up

Export Citation Format

Share Document