Female detection of the synthetic sex pheromone contributes to the efficacy of mating disruption of the European grapevine moth, Lobesia botrana

2014 ◽  
Vol 71 (2) ◽  
pp. 316-322 ◽  
Author(s):  
Ally R Harari ◽  
Tirtza Zahavi ◽  
Hadass Steinitz
Keyword(s):  
Sex Pheromone ◽  
Mating Disruption ◽  
Lobesia Botrana ◽  
European Grapevine Moth ◽  
Synthetic Sex Pheromone ◽  
Grapevine Moth

scholarly journals Airborne Pheromone Quantification in Treated Vineyards with Different Mating Disruption Dispensers against Lobesia botrana

Insects ◽  
2020 ◽  
Vol 11 (5) ◽  
pp. 289
Author(s):  
Aitor Gavara ◽  
Sandra Vacas ◽  
Ismael Navarro ◽  
Jaime Primo ◽  
Vicente Navarro-Llopis
Keyword(s):  
Mating Disruption ◽  
Lobesia Botrana ◽  
Spectroscopic Methods ◽  
Concentration Profiles ◽  
European Grapevine Moth ◽  
Pheromone Concentration ◽  
Crop Cycle ◽  
Competitive Mechanisms ◽  
Grapevine Moth ◽  
Airborne Pheromone

Mating disruption (MD) is widely used against the European grapevine moth (EGVM), Lobesia botrana (Denis and Schiffermüller; Lepidoptera: Tortricidae), by installing passive dispensers or aerosol devices. The present work reports a new sampling and quantification methodology to obtain absolute data about field airborne pheromone concentration based on air samplings and sensitive chromatographic-spectroscopic methods. Samplings were performed in fields treated with passive dispensers or aerosol devices at different moments throughout the crop cycle to study how they act and how the disruption is triggered. Moreover, pheromone adsorption and releasing capacity of vine leaves were studied to elucidate their role in the disruption. Although both types of dispensers were effective in limiting the damage inflicted by EGVM, they performed differently and provided different airborne pheromone concentration profiles. Results also proved that leaves were able to adsorb and release part of the airborne pheromone acting as subsequent and additional pheromone sources. This fact could explain the different concentration profiles. Moreover, our results suggest that lower pheromone emission than that of the current passive dispensers still could provide an adequate performance in the field. Competitive mechanisms involved in MD using both dispensers, the dynamics of the airborne pheromone throughout the time and the importance of the canopy are discussed.

scholarly journals Biosynthesis of the Sex Pheromone Component (E,Z)-7,9-Dodecadienyl Acetate in the European Grapevine Moth, Lobesia botrana, Involving ∆11 Desaturation and an Elusive ∆7 Desaturase

2021 ◽  
Author(s):  
Bao-Jian Ding ◽  
Yi-Han Xia ◽  
Hong-Lei Wang ◽  
Fredrik Andersson ◽  
Erik Hedenström ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Double Bond ◽  
Sex Pheromone ◽  
Fatty Acyl ◽  
Lobesia Botrana ◽  
Pheromone Component ◽  
Functional Assay ◽  
Tetradecanoic Acid ◽  
European Grapevine Moth ◽  
Grapevine Moth

AbstractThe European grapevine moth, Lobesia botrana, uses (E,Z)-7,9-dodecadienyl acetate as its major sex pheromone component. Through in vivo labeling experiments we demonstrated that the doubly unsaturated pheromone component is produced by ∆11 desaturation of tetradecanoic acid, followed by chain shortening of (Z)-11-tetradecenoic acid to (Z)-9-dodecenoic acid, and subsequently introduction of the second double bond by an unknown ∆7 desaturase, before final reduction and acetylation. By sequencing and analyzing the transcriptome of female pheromone glands of L. botrana, we obtained 41 candidate genes that may be involved in sex pheromone production, including the genes encoding 17 fatty acyl desaturases, 13 fatty acyl reductases, 1 fatty acid synthase, 3 acyl-CoA oxidases, 1 acetyl-CoA carboxylase, 4 fatty acid transport proteins and 2 acyl-CoA binding proteins. A functional assay of desaturase and acyl-CoA oxidase gene candidates in yeast and insect cell (Sf9) heterologous expression systems revealed that Lbo_PPTQ encodes a ∆11 desaturase producing (Z)-11-tetradecenoic acid from tetradecanoic acid. Further, Lbo_31670 and Lbo_49602 encode two acyl-CoA oxidases that may produce (Z)-9-dodecenoic acid by chain shortening (Z)-11-tetradecenoic acid. The gene encoding the enzyme introducing the E7 double bond into (Z)-9-dodecenoic acid remains elusive even though we assayed 17 candidate desaturases in the two heterologous systems.

scholarly journals Grape variety affects larval performance and also female reproductive performance of the European grapevine moth Lobesia botrana (Lepidoptera: Tortricidae)

2006 ◽  
Vol 96 (2) ◽  
pp. 205-212 ◽  
Author(s):  
J. Moreau ◽  
B. Benrey ◽  
D. Thiéry
Keyword(s):  
Host Plant ◽  
Adult Emergence ◽  
Lobesia Botrana ◽  
Larval Performance ◽  
European Grapevine Moth ◽  
Wild Grape ◽  
Larval Host ◽  
Fitness Index ◽  
Grapevine Moth

AbstractFor insect herbivores, the quality of the larval host plant is a key determinant of fitness. Therefore, insect populations are supposed to be positively correlated with the nutritional quality of their host plant. This study aimed to determine if and how different varieties of grapes (including the wild grape Lambrusque) affect both larval and adult performance of the polyphagous European grapevine moth Lobesia botrana (Denis & Schiffermüller). Significant differences were found in larval development time, but not in pupal mass, adult emergence rate, or sex ratio. Although the fecundity of females is not different among varieties, females fed on some varieties produced eggs of different sizes which are correlated to their fertility. Thus, females adapt resource allocation to eggs depending on their diet as larvae. Using a fitness index, the average reproductive output was found to be highest for females reared on cv. Chardonnay. Females reared on wild grape produced a fitness index identical to the cultivated grapes. However, Lambrusque and Gewurztraminer separate themselves from the cultivated varieties according to our discriminant analyses. It is emphasized, through this study, that cultivars fed on by larvae should be considered in the population dynamics of L. botrana and that egg number is insufficient to determine host plant quality.

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