The role of glutathioneS-transferases in the detoxification of some organophosphorus insecticides in larvae and pupae of the yellow mealworm,Tenebrio molitor (Coleoptera: Tenebrionidae)

2001 ◽  
Vol 57 (6) ◽  
pp. 501-508 ◽  
Author(s):  
Iason Kostaropoulos ◽  
Athanasios I Papadopoulos ◽  
Athanasios Metaxakis ◽  
Evridiki Boukouvala ◽  
Euphemia Papadopoulou-Mourkidou
Keyword(s):  
Tenebrio Molitor ◽  
Organophosphorus Insecticides ◽  
Yellow Mealworm ◽  
Coleoptera Tenebrionidae

Dietary fatty acids influence the growth and fatty acid composition of the yellow mealworm Tenebrio molitor (Coleoptera: Tenebrionidae)

Lipids ◽  
2017 ◽  
Vol 52 (3) ◽  
pp. 285-294 ◽  
Author(s):  
Elena Dreassi ◽  
Annarita Cito ◽  
Assunta Zanfini ◽  
Lara Materozzi ◽  
Maurizio Botta ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Acid Composition ◽  
Tenebrio Molitor ◽  
Dietary Fatty Acids ◽  
Yellow Mealworm ◽  
Coleoptera Tenebrionidae

Impact of density, reproduction period and age on fecundity of the yellow mealworm Tenebrio molitor (Coleoptera: Tenebrionidae)

2018 ◽  
Vol 4 (1) ◽  
pp. 43-50 ◽  
Author(s):  
I.E. Berggreen ◽  
J. Offenberg ◽  
M. Calis ◽  
L.-H. Heckmann
Keyword(s):  
Tenebrio Molitor ◽  
Adult Density ◽  
Yellow Mealworm ◽  
Reproduction Period ◽  
Per Capita ◽  
The Impact ◽  
Old Females ◽  
Effect Of Age ◽  
Coleoptera Tenebrionidae

The impact of adult density, reproduction period (days in the same container) and age on reproduction of Tenebrio molitor Linnaeus (Coleoptera: Tenebrionidae) were studied. Data were analysed as total number of larvae in each container (38 cm2) and as daily reproduction per capita. The study was divided in two experiments. Experiment 1 examined the influence of four densities (0.11, 0.21, 0.42 and 0.84 beetles/cm2) and five reproduction periods (1, 2, 3, 4 and 6 days) on reproduction. Experiment 2 examined the effect of age (30-92 days) and three densities (0.11, 0.21 and 0.32 beetles/cm2) on reproduction. The total number of larvae per container was significantly affected by reproduction period and adult density, attaining the highest output at the longest reproduction period of 6 days (214±101 larvae) and highest density of 0.84 beetles/cm2 (215±112 larvae). Daily per capita reproduction, however, was highest at the second lowest density of 0.21 beetles/cm2 (8.58±3.82 larvae/female/day) and shortest reproduction period of 1 day (8.34±4.68 larvae/female/day). In experiment 2, age had a significant effect on reproduction both as total number of larvae per container and number of larvae per female per day. The highest reproduction was found when beetles were 30 days old, but reproduction was in general highest for 13-29 days old females (experiment 1). For production context the highest density of beetles provides highest output of larvae, and beetles are not expected to be stressed under these conditions.

Biosynthesis and Secretion of Serine Peptidase SerP38 from Tenebrio molitor in the Yeast Komagataella kurtzmanii

2020 ◽  
Vol 36 (6) ◽  
pp. 78-86
Author(s):  
P.I. Akentyev ◽  
I.I. Gubaidullin ◽  
N.I. Zhiganov ◽  
V.F. Tereshchenkova ◽  
E.N. Elpidina ◽  
...  
Keyword(s):  
Culture Medium ◽  
Polypeptide Chain ◽  
Tenebrio Molitor ◽  
Enzyme Secretion ◽  
Trypsin Treatment ◽  
Yellow Mealworm ◽  
Serine Peptidase

A strain of the Komagataella kurtzmanii yeast, a producer of recombinant peptidase SerP38 from the yellow mealworm Tenebrio molitor, has been obtained. The level of the pro-enzyme secretion was 20-50 mg/L. It was shown that, during secretion in yeast, the target His6-tagged protein was produced in two forms. One of them was a monomer that was efficiently purified by Ni-NTA chromatography and then activated with trypsin. Another form accumulated in the culture medium as oligomers prone to aggregation in the presence of Ni2+ ions and was not activated by trypsin treatment. Aggregation is likely the result of incorrect folding of the polypeptide chain. Tenebrio molitor, S1 family serine peptidase, SerP38, yeast, Komagataella kurtzmanii, ion-dependent aggregation

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