Cross-resistance between azinphos-methyl and acetamiprid in populations of codling moth,Cydia pomonella(L.) (Lepidoptera: Tortricidae), from Washington State

2010 ◽  
pp. n/a-n/a ◽  
Author(s):  
Alan L Knight
Keyword(s):  
Cydia Pomonella ◽  
Codling Moth ◽  
Washington State ◽  
Cross Resistance ◽  
Azinphos Methyl

The toxicity of insecticides to larvae of the codling moth, Cydia pomonella (L.). II.—Maintenance of a toxic deposit in the field

1965 ◽  
Vol 56 (2) ◽  
pp. 377-388 ◽  
Author(s):  
M. Gratwick ◽  
J. M. Sillibourne ◽  
R. P. Tew
Keyword(s):  
Larval Mortality ◽  
Cydia Pomonella ◽  
Field Performance ◽  
Heavy Rain ◽  
Codling Moth ◽  
Chemical Methods ◽  
Azinphos Methyl ◽  
Field Control ◽  
Wettable Powder ◽  
Rates Of Decay

The persistence, as assessed by biological and chemical methods, of field deposits on apples from two spray programmes, each of DDT, carbaryl or azinphos-methyl, was compared throughout eight weeks following the first application in an orchard in south-eastern England. Newly emerged larvae of the codling moth, Cydia pomonella (L.), were used to assess, in the laboratory, the biological activity of the deposits. The spray programmes studied were (1) two applications of conventional concentrations (i.e., 0·1 per cent, for DDT and for carbaryl, 0·04 per cent, for azinphos-methyl) three weeks apart and (2) three applications of half these concentrations at two-weekly intervals. DDT wettable powder, carbaryl and azinphos-methyl were compared in 1961, DDT emulsion, DDT wettable powder and carbaryl in 1963. Results of biological and chemical assessment of the deposits are presented graphically. Over-all, the performance of the three-application programmes was as satisfactory as that of the two-application programmes, although a smaller amount of chemical was used. The serious reduction in deposit caused by heavy rain shortly after the first application of DDT wettable powder and of carbaryl in 1961 is noted. In the absence of heavy rain, carbaryl was biologically the most persistent, but the best performances of the other chemicals were almost as good as that of carbaryl.LD50 values of fresh deposits obtained by field spraying were shown to be similar to those obtained by laboratory dipping.Comparison of the toxicity of fresh and weathered field deposits of similar magnitude indicated that the biologically available proportion of a DDT deposit from either formulation decreases as a result of weathering. This phenomenon was not found to occur with carbaryl and either not at all or to a much lesser extent with azinphos-methyl.The deposits obtained in the comparison of the spray programmes were separated into their fresh and aged components, so that differences in the contribution to the final deposit provided by each application could be seen. With the very persistent DDT emulsion, only about half of the six-week deposits was composed of chemical from the final application, but, with the much less persistent carbaryl and azinphos methyl, the six-week deposits were almost entirely derived from the final application. By substitution in an equation to obtain the rates of decay of the deposits from the three-application programmes of all chemicals, it is shown that deposits from the second and third applications were, in all cases, more persistent than those from the first. Possible reasons for this difference are discussed.The significance, in terms of field control, of the results for the larvicidal activity of the deposits from the different spray programmes is indicated. It is concluded that data on the LD90 of insecticidal deposits on apples, together with measurement of their persistence in the field, can be used to assess the probable field performance of codling-moth insecticides and to determine the most efficient means of employing them, although the deposit level that ensures 90 per cent, larval mortality in the field is higher than the LD90 determined in the laboratory.

scholarly journals Assessment of control strategies against Cydia pomonella (L.) in Morocco

2016 ◽  
Vol 56 (1) ◽  
pp. 82-88 ◽  
Author(s):  
Salma El Iraqui ◽  
M’hamed Hmimina
Keyword(s):  
Chemical Control ◽  
Control Strategies ◽  
Cydia Pomonella ◽  
Codling Moth ◽  
Average Frequency ◽  
Cross Resistance ◽  
Moth Population ◽  
Action Threshold ◽  
Two Parameters ◽  
Neonate Larvae

Abstract The codling moth, Cydia pomonella (L.), is the key pest of apple production worldwide. In Morocco, there is a sustainable presence of codling moth causing considerable damage in apple orchards despite frequent applications of broad spectrum insecticides. For 12 years, sexual trapping and chemical control were performed and the development of the codling moth population was analysed in an orchard which was in the region of Azrou. The efficacy of some insecticides (azinphos-methyl, chlorpyriphos-ethyl, diflubenzuron, thiacloprid, methoxyfenozide, spinosad, and deltamethrin) was also evaluated on neonate larvae and compared with a laboratory sensitive strain. This procedure was done to assess an eventual resistance in Moroccan populations. The action threshold was usually exceeded, leading to an intensive chemical control, with an average frequency of 9 to 13 days. The chemical control was done according to the action persistence time of the insecticides and the trap captures. However, those two parameters are compromised in Moroccan conditions because of the high summer temperatures which disrupt the action of insecticides and exacerbate populations. The pheromone traps may become ineffective and useless. Neonate larvae were resistant to five insecticides out of seven. Such results suggest the presence of a cross resistance in local strains. Overall, the insect resistance, the functioning of the sexual traps, and some insecticides properties (persistence action, pre-harvest interval) are the key factors that could explain the failure to control these moths under Moroccan conditions.

scholarly journals Cross-Resistance of the Codling Moth against Different Isolates of Cydia pomonella Granulovirus Is Caused by Two Different but Genetically Linked Resistance Mechanisms

Viruses ◽  
2021 ◽  
Vol 13 (10) ◽  
pp. 1952
Author(s):  
Annette J. Sauer ◽  
Eva Fritsch ◽  
Karin Undorf-Spahn ◽  
Kento Iwata ◽  
Regina G. Kleespies ◽  
...  
Keyword(s):  
Biological Control Agent ◽  
Cydia Pomonella ◽  
Codling Moth ◽  
Resistance Mechanisms ◽  
Cross Resistance ◽  
Viral Gene ◽  
Type I ◽  
Type Ii ◽  
Cydia Pomonella Granulovirus ◽  
Type Ii Resistance

Cydia pomonella granulovirus (CpGV) is a widely used biological control agent of the codling moth. Recently, however, the codling moth has developed different types of field resistance against CpGV isolates. Whereas type I resistance is Z chromosomal inherited and targeted at the viral gene pe38 of isolate CpGV-M, type II resistance is autosomal inherited and targeted against isolates CpGV-M and CpGV-S. Here, we report that mixtures of CpGV-M and CpGV-S fail to break type II resistance and is expressed at all larval stages. Budded virus (BV) injection experiments circumventing initial midgut infection provided evidence that resistance against CpGV-S is midgut-related, though fluorescence dequenching assay using rhodamine-18 labeled occlusion derived viruses (ODV) could not fully elucidate whether the receptor binding or an intracellular midgut factor is involved. From our peroral and intra-hemocoel infection experiments, we conclude that two different (but genetically linked) resistance mechanisms are responsible for type II resistance in the codling moth: resistance against CpGV-M is systemic whereas a second and/or additional resistance mechanism against CpGV-S is located in the midgut of CpR5M larvae.

scholarly journals Economic Impact of a Potential Expansion of Pest Infestation: Apple Maggot in Washington State

2018 ◽  
Vol 28 (5) ◽  
pp. 651-659
Author(s):  
Suzette P. Galinato ◽  
R. Karina Gallardo ◽  
David M. Granatstein ◽  
Mike Willett
Keyword(s):  
Insect Pest ◽  
Cydia Pomonella ◽  
Codling Moth ◽  
Commercial Production ◽  
Washington State ◽  
Rhagoletis Pomonella ◽  
Pest Infestation ◽  
Additional Costs ◽  
Apple Production ◽  
Production Areas

Apple maggot (Rhagoletis pomonella) is an insect pest of apple (Malus domestica) that is currently limited in extent in the commercial production areas of Washington State thanks to a quarantine program. We estimate the costs to the Washington economy if this pest were to spread more widely. Apple maggot control costs are related to the pressure of codling moth (Cydia pomonella), the most prevalent insect pest in commercial apple production in Washington State. It was found that the losses for the Washington apple industry’s range from $510 million to $557 million, depending on the codling moth pressure. Our findings underscore the importance of an efficient quarantine program that minimized the risk of spreading the pest along with additional costs associated with quarantined areas.

Efficacy of Codling Moth Granulovirus: Effect of Adjuvants on Persistence of Activity and Comparison With Other Larvicides in a Pacific Northwest Apple Orchard

2004 ◽  
Vol 39 (4) ◽  
pp. 500-513 ◽  
Author(s):  
Lawrence A. Lacey ◽  
Steven Arthurs ◽  
Alan Knight ◽  
Kimberly Becker ◽  
Heather Headrick
Keyword(s):  
Pacific Northwest ◽  
Broad Spectrum ◽  
Cydia Pomonella ◽  
Microbial Control ◽  
Residual Activity ◽  
Codling Moth ◽  
Apple Orchard ◽  
Azinphos Methyl ◽  
Control Options ◽  
Neonate Larvae

Control of codling moth, Cydia pomonella (L), in conventional orchards has relied heavily on broad spectrum insecticides such as azinphos-methyl (Guthion®, Bayer CropScience, Research Triangle Park, NC). Alternative control options are needed for a variety of reasons, including environmental impact and worker and food safety concerns. Microbial control agents such as the codling moth granulovirus (CpGV) offer alternatives to conventional insecticides for the control of codling moth. Six weekly applications of the label rate (1 L/ha) of the Carpovirusine® formulation of CpGV in an experimental orchard naturally infested with codling moth provided control of first generation codling moth that was comparable to that of larvicidal oil and azinphos-methyl. Although the number of codling moth entries in fruit that were treated with virus alone was similar to that of control trees, the number of deep entries and the number of living codling moth larvae were significantly reduced on CpGV treated fruit. Despite blemishing, virus-treated fruit with minute entries were suitable for consumption or for processing. Studies on the residual activity of Carpovirusine revealed a steady decline in virus activity 1 to 3 d following application. The use of two adjuvants, Nu-Film-17® and Raynox®, did not protect virus from solar inactivation. Among the biological control options available for codling moth, CpGV provides effective and selective control of neonate larvae. Its use in lieu of broad spectrum insecticides will contribute significantly to the conservation of other natural enemies in the orchard agroecosystem.

scholarly journals Novel resistance to Cydia pomonella granulovirus (CpGV) in codling moth shows autosomal and dominant inheritance and confers cross-resistance to different CpGV genome groups

PLoS ONE ◽  
2017 ◽  
Vol 12 (6) ◽  
pp. e0179157 ◽  
Author(s):  
Annette J. Sauer ◽  
Eva Fritsch ◽  
Karin Undorf-Spahn ◽  
Petr Nguyen ◽  
Frantisek Marec ◽  
...  
Keyword(s):  
Cydia Pomonella ◽  
Codling Moth ◽  
Cross Resistance ◽  
Dominant Inheritance ◽  
Cydia Pomonella Granulovirus

The toxicity of insecticides to larvae of the codling moth, Cydia pomonella (L.). I.—Intrinsic toxicity and persistence

1965 ◽  
Vol 56 (2) ◽  
pp. 367-376 ◽  
Author(s):  
M. Gratwick ◽  
J. M. Sillibourne ◽  
R. P. Tew
Keyword(s):  
Half Life ◽  
Cydia Pomonella ◽  
Codling Moth ◽  
Lead Arsenate ◽  
Azinphos Methyl ◽  
Wettable Powder ◽  
Cent Mortality ◽  
Biological Tests ◽  
Life Values ◽  
Good Agreement

The deposits found on apples at various times after application of 11 insecticides were determined by chemical analysis of samples from an orchard in south-eastern England sprayed in 1959 and 1960. The results, from samples taken simultaneously, of studies of the effectiveness of these and other chemicals for control of the codling moth, Cydia pomonella (L.), have already been reported.The period in which 50 per cent, of an initial deposit disappeared in the field (half-life) was calculated from the chemical data and used as a measure of persistence. For comparisons of intrinsic toxicity, the levels of fresh deposits causing 50 per cent, mortality of newly emerged codling-moth larvae (LD50) were determined in the laboratory by chemical and biological tests on apples dipped in a range of concentrations of commercial formulations. The LD50's, in μg./cm.2, were less than 0·025 for carbophenothion, parathion and diazinon, between 0·025 and 0·05 for azinphos-methyl and -ethyl, between 0·05 and 0·10 for malathion, DDT and carbaryl, between 0·1 and 0·5 for DDD and dimethoate, between 0·5 and 1·5 for lead arsenate and above 1·5 for phenkapton.Chemicals with an LD50 exceeding about 0·1 μg./cm.2 were considered not worth further investigation as codling-moth larvicides. The LD90's of the remainder were found and were taken as the minimum deposits that would have an adequate effect from the standpoint of control (minimum effective levels). From the half-life values, the periods for which the deposits found in the orchard in 1960 should have remained above the minimum effective levels were calculated; these were 24 days for DDT emulsion, 22 for DDT wettable powder, 15 for carbophenothion, 14 for carbaryl, 12 for azinphos-ethyl, 11 for parathion, 7 for malathion and 6 for diazinon. They were in good agreement with the results of the biological tests except in the case of DDT emulsion, the effectiveness of which was unexpectedly short-lived.

scholarly journals Genome-Wide Selective Signature Analysis Revealed Insecticide Resistance Mechanisms in Cydia pomonella

Insects ◽  
2021 ◽  
Vol 13 (1) ◽  
pp. 2
Author(s):  
Wen-Ting Dai ◽  
Jin Li ◽  
Li-Ping Ban
Keyword(s):  
Insecticide Resistance ◽  
Insect Pest ◽  
Glycine Receptor ◽  
Cydia Pomonella ◽  
Resistance Management ◽  
Codling Moth ◽  
Resistance Mechanisms ◽  
Expression Level ◽  
Invasive Pest ◽  
Cross Resistance

The codling moth, Cydia pomonella L. (Lepidoptera, Tortricidae), is a serious invasive pest of pome fruits. Currently, C. pomonella management mainly relies on the application of insecticides, which have driven the development of resistance in the insect. Understanding the genetic mechanisms of insecticide resistance is of great significance for developing new pest resistance management techniques and formulating effective resistance management strategies. Using existing genome resequencing data, we performed selective sweep analysis by comparing two resistant strains and one susceptible strain of the insect pest and identified seven genes, among which, two (glycine receptor and glutamate receptor) were under strong insecticide selection, suggesting their functional importance in insecticide resistance. We also found that eight genes including CYP6B2, CYP307a1, 5-hydroxytryptamine receptor, cuticle protein, and acetylcholinesterase, are potentially involved in cross-resistance to azinphos-methyl and deltamethrin. Moreover, among several P450s identified as positively selected genes, CYP6B2, CYP4C1, and CYP4d2 showed the highest expression level in larva compared to other stages tested, and CYP6B2 also showed the highest expression level in midgut, supporting the roles they may play in insecticide metabolism. Our results provide several potential genes that can be studied further to advance understanding of complexity of insecticide resistance mechanisms in C. pomonella.

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