scholarly journals The G protein-coupled estrogen receptor agonist, G-1, attenuates BK channel activation in cerebral arterial smooth muscle cells

2018 ◽  
Vol 6 (4) ◽  
pp. e00409 ◽  
Author(s):  
Kirk W. Evanson ◽  
Jacob A. Goldsmith ◽  
Payal Ghosh ◽  
Michael D. Delp
Keyword(s):  
Estrogen Receptor ◽  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
G Protein ◽  
Receptor Agonist ◽  
Muscle Cells ◽  
Bk Channel ◽  
Channel Activation ◽  
Arterial Smooth Muscle Cells ◽  
G Protein Coupled

Overexpression of G Protein-Coupled Receptor Kinase-2 in Smooth Muscle Cells Reduces Neointimal Hyperplasia

2002 ◽  
Vol 34 (10) ◽  
pp. 1399-1409 ◽  
Author(s):  
Karsten Peppel ◽  
Lisheng Zhang ◽  
Tam T.T. Huynh ◽  
Xuewei Huang ◽  
Anne Jacobson ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
G Protein ◽  
Neointimal Hyperplasia ◽  
Muscle Cells ◽  
Receptor Kinase ◽  
G Protein Coupled Receptor ◽  
G Protein Coupled

scholarly journals Selective Activation of Nuclear Phospholipase D-1 by G Protein–Coupled Receptor Agonists in Vascular Smooth Muscle Cells

2006 ◽  
Vol 99 (2) ◽  
pp. 132-139 ◽  
Author(s):  
Stéphanie Gayral ◽  
Paul Déléris ◽  
Karine Laulagnier ◽  
Muriel Laffargue ◽  
Jean-Pierre Salles ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
G Protein ◽  
Vascular Smooth Muscle Cells ◽  
Phospholipase D ◽  
Muscle Cells ◽  
G Protein Coupled Receptor ◽  
Selective Activation ◽  
G Protein Coupled

scholarly journals Type 1 IP3 receptors activate BKCa channels via local molecular coupling in arterial smooth muscle cells

2010 ◽  
Vol 136 (3) ◽  
pp. 283-291 ◽  
Author(s):  
Guiling Zhao ◽  
Zachary P. Neeb ◽  
M. Dennis Leo ◽  
Judith Pachuau ◽  
Adebowale Adebiyi ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Muscle Cells ◽  
Bkca Channel ◽  
Ip3 Receptors ◽  
Arterial Smooth Muscle ◽  
Bkca Channels ◽  
Channel Activation ◽  
Arterial Smooth Muscle Cells

Plasma membrane large-conductance Ca2+-activated K+ (BKCa) channels and sarcoplasmic reticulum inositol 1,4,5-trisphosphate (IP3) receptors (IP3Rs) are expressed in a wide variety of cell types, including arterial smooth muscle cells. Here, we studied BKCa channel regulation by IP3 and IP3Rs in rat and mouse cerebral artery smooth muscle cells. IP3 activated BKCa channels both in intact cells and in excised inside-out membrane patches. IP3 caused concentration-dependent BKCa channel activation with an apparent dissociation constant (Kd) of ∼4 µM at physiological voltage (−40 mV) and intracellular Ca2+ concentration ([Ca2+]i; 10 µM). IP3 also caused a leftward-shift in BKCa channel apparent Ca2+ sensitivity and reduced the Kd for free [Ca2+]i from ∼20 to 12 µM, but did not alter the slope or maximal Po. BAPTA, a fast Ca2+ buffer, or an elevation in extracellular Ca2+ concentration did not alter IP3-induced BKCa channel activation. Heparin, an IP3R inhibitor, and a monoclonal type 1 IP3R (IP3R1) antibody blocked IP3-induced BKCa channel activation. Adenophostin A, an IP3R agonist, also activated BKCa channels. IP3 activated BKCa channels in inside-out patches from wild-type (IP3R1+/+) mouse arterial smooth muscle cells, but had no effect on BKCa channels of IP3R1-deficient (IP3R1−/−) mice. Immunofluorescence resonance energy transfer microscopy indicated that IP3R1 is located in close spatial proximity to BKCa α subunits. The IP3R1 monoclonal antibody coimmunoprecipitated IP3R1 and BKCa channel α and β1 subunits from cerebral arteries. In summary, data indicate that IP3R1 activation elevates BKCa channel apparent Ca2+ sensitivity through local molecular coupling in arterial smooth muscle cells.

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