scholarly journals Inhibition of RhoA/Rho kinase pathway and smooth muscle contraction by hydrogen sulfide

2017 ◽  
Vol 5 (5) ◽  
pp. e00343 ◽  
Author(s):  
Ancy D. Nalli ◽  
Hongxia Wang ◽  
Sayak Bhattacharya ◽  
Bryan A. Blakeney ◽  
Karnam S. Murthy
Keyword(s):  
Smooth Muscle ◽  
Hydrogen Sulfide ◽  
Muscle Contraction ◽  
Rho Kinase ◽  
Smooth Muscle Contraction ◽  
Kinase Pathway

Effect of oxidative stress on Rho kinase II and smooth muscle contraction in rat stomach

2015 ◽  
Vol 93 (6) ◽  
pp. 405-411 ◽  
Author(s):  
Othman Al-Shboul ◽  
Ayman Mustafa
Keyword(s):  
Oxidative Stress ◽  
Smooth Muscle ◽  
Muscle Contraction ◽  
Muscle Cell ◽  
Rho Kinase ◽  
Smooth Muscle Contraction ◽  
Enzyme Linked Immunosorbent Assay ◽  
Rat Stomach ◽  
Cell Contraction ◽  
Kinase Pathway

Recent studies have shown that both Rho kinase signaling and oxidative stress are involved in the pathogenesis of a number of human diseases, such as diabetes mellitus, hypertension, and atherosclerosis. However, very little is known about the effect of oxidative stress on the gastrointestinal (GI) smooth muscle Rho kinase pathway. The aim of the current study was to investigate the effect of oxidative stress on Rho kinase II and muscle contraction in rat stomach. The peroxynitrite donor 3-morpholinosydnonimine (SIN-1), hydrogen peroxide (H2O2), and peroxynitrite were used to induce oxidative stress. Rho kinase II expression and ACh-induced activity were measured in control and oxidant-treated cells via specifically designed enzyme-linked immunosorbent assay (ELISA) and activity assay kits, respectively. Single smooth muscle cell contraction was measured via scanning micrometry in the presence or absence of the Rho kinase blocker, Y-27632 dihydrochloride. All oxidant agents significantly increased ACh-induced Rho kinase II activity without affecting its expression level. Most important, oxidative stress induced by all three agents augmented ACh-stimulated muscle cell contraction, which was significantly inhibited by Y-27632. In conclusion, oxidative stress activates Rho kinase II and enhances contraction in rat gastric muscle, suggesting an important role in GI motility disorders associated with oxidative stress.

Amitriptyline attenuates smooth muscle contraction of rat trachea through the Rho-kinase pathway

2007 ◽  
Vol 24 (Supplement 39) ◽  
pp. 126
Author(s):  
O. Shibata ◽  
K. Nishioka ◽  
M. Yamaguchi ◽  
T. Makita ◽  
K. Sumikawa
Keyword(s):  
Smooth Muscle ◽  
Muscle Contraction ◽  
Rho Kinase ◽  
Smooth Muscle Contraction ◽  
Rat Trachea ◽  
Kinase Pathway

Anticholinesterase Drugs Stimulate Smooth Muscle Contraction of the Rat Trachea Through the Rho-Kinase Pathway

2006 ◽  
Vol 102 (4) ◽  
pp. 1121-1126 ◽  
Author(s):  
Osamu Shibata ◽  
Masataka Saito ◽  
Maki Yoshimura ◽  
Masakazu Yamaguchi ◽  
Kenji Nishioka ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Muscle Contraction ◽  
Rho Kinase ◽  
Smooth Muscle Contraction ◽  
Rat Trachea ◽  
Kinase Pathway

Role of the RhoA/Rho-kinase pathway in the regulation of pulmonary vasoconstrictor function

2010 ◽  
Vol 88 (1) ◽  
pp. 1-8 ◽  
Author(s):  
Bobby D. Nossaman ◽  
Vaughn E. Nossaman ◽  
Subramanyam N. Murthy ◽  
Philip J. Kadowitz
Keyword(s):  
Smooth Muscle ◽  
Muscle Contraction ◽  
Calcium Binding ◽  
Rho Kinase ◽  
Smooth Muscle Contraction ◽  
Downstream Effector ◽  
Downstream Effectors ◽  
Vascular Beds ◽  
Kinase Pathway

Calcium is the major intracellular messenger that triggers smooth muscle contraction. The study of calcium-binding proteins, such as calmodulin and its downstream effectors, reveals critical regulation of smooth muscle contraction by protein kinases and phosphatases. Moreover, the small GTP-binding protein RhoA and its downstream effector protein, Rho-kinase, have been shown to play a novel role in the regulation of smooth muscle contraction. Studies have shown that the activation of Rho-kinase is involved in the development of endothelial dysfunction, inflammation, restenosis, and increased vascular tone in a number of cardiovascular disorders. Because inhibitors of this pathway promote vasodilation independent of the mechanism that increases vasoconstrictor tone, it is our hypothesis that Rho-kinase is constitutively active in regulating vasoconstrictor tone in the pulmonary and systemic vascular beds. Studies in the literature suggest that the RhoA/Rho-kinase pathway has an important role in the pathogenesis of pulmonary hypertension.

scholarly journals Allergic sensitization enhances the contribution of Rho-kinase to airway smooth muscle contraction

2004 ◽  
Vol 143 (4) ◽  
pp. 477-484 ◽  
Author(s):  
Dedmer Schaafsma ◽  
Reinoud Gosens ◽  
I Sophie T Bos ◽  
Herman Meurs ◽  
Johan Zaagsma ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Muscle Contraction ◽  
Airway Smooth Muscle ◽  
Allergic Sensitization ◽  
Rho Kinase ◽  
Smooth Muscle Contraction

Involvement of RhoA/Rho-kinase in l-cysteine/H2S pathway-induced inhibition of agonist-mediated corpus cavernosal smooth muscle contraction

Nitric Oxide ◽  
2019 ◽  
Vol 85 ◽  
pp. 54-60 ◽  
Author(s):  
Fatma Aydinoglu ◽  
Elif Özveren Adıbelli ◽  
Didem Yılmaz-Oral ◽  
Nuran Ogulener
Keyword(s):  
Smooth Muscle ◽  
Muscle Contraction ◽  
Rho Kinase ◽  
Smooth Muscle Contraction
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