scholarly journals The red‐vine‐leaf extract AS195 increases nitric oxide synthase–dependent nitric oxide generation and decreases oxidative stress in endothelial and red blood cells

2016 ◽  
Vol 4 (1) ◽  
Author(s):  
Marijke Grau ◽  
Birgit Bölck ◽  
Daniel Alexander Bizjak ◽  
Christina Julia Annika Stabenow ◽  
Wilhelm Bloch
Keyword(s):  
Oxidative Stress ◽  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Leaf Extract ◽  
Oxide Synthase

scholarly journals Mature Twin Neonates Exhibit Oxidative Stress via Nitric Oxide Synthase Dysfunctionality: A Prognostic Stress Marker in the Red Blood Cells and Umbilical Cord Vessels

Antioxidants ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 845
Author(s):  
Payal Chakraborty ◽  
Krisztina N. Dugmonits ◽  
Hajnalka Orvos ◽  
Edit Hermesz
Keyword(s):  
Oxidative Stress ◽  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Red Blood Cells ◽  
Umbilical Cord ◽  
Blood Cells ◽  
Low Birthweight ◽  
Multiple Pregnancy ◽  
No Production ◽  
Oxide Synthase

Intrauterine hypoxic condition increases the generation of reactive oxygen species and fetal oxidative stress. Multiple pregnancy always bears an additional oxidative stress condition with severe complications, such as prematurity, structural abnormalities, delayed development and low birthweight. The umbilical cord (UC) vessels, along with circulating fetal red blood cells (RBCs), highly determine the oxygenation status of fetus and regulate the feto-placental circulation. As UC lacks innervation, the activation of the endothelial nitric oxide synthase (NOS3) is fundamental for proper NO production. Therefore, we aimed to study the NOS3 activation pathways along with damages to macromolecules in the endothelium of UC vessels and RBCs of mature non-discordant twins, in connection to major differences in their birth weight. We provide evidence that, under severe hypoxic conditions such as twin pregnancy, the NOS3-related NO production pathways are altered both in UC vessels and RBCs; moreover, the extent of changes is highly birthweight-specific. Furthermore, macromolecular damages are prominent in the RBCs and arteries compared to the vein, with a similar increase in the Arginase1 level, which is believed to play a role in NOS3 functionality, resulting in endothelial dysfunctionality, which might have relevance to the major etiologies of cardiovascular diseases in later life.

scholarly journals Shear stress activation of nitric oxide synthase and increased nitric oxide levels in human red blood cells

Nitric Oxide ◽  
2011 ◽  
Vol 24 (4) ◽  
pp. 184-191 ◽  
Author(s):  
Pinar Ulker ◽  
Nazmi Yaras ◽  
Ozlem Yalcin ◽  
Ciler Celik-Ozenci ◽  
Paul C. Johnson ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Shear Stress ◽  
Nitric Oxide Synthase ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Human Red Blood Cells ◽  
Oxide Synthase ◽  
Stress Activation

scholarly journals Mechanical perturbations trigger endothelial nitric oxide synthase activity in human red blood cells

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Shunmugan Nagarajan ◽  
Rajendran Kadarkarai Raj ◽  
Venkatesan Saravanakumar ◽  
Uma Maheswari Balaguru ◽  
Jyotirmaya Behera ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Endothelial Nitric Oxide Synthase ◽  
Human Red Blood Cells ◽  
Oxide Synthase ◽  
Endothelial Nitric Oxide ◽  
Nitric Oxide Synthase Activity

scholarly journals Nuclear factor-kB and nitric oxide synthases in red blood cells: good or bad in obesity? A preliminary study

2020 ◽  
Vol 64 (1) ◽  
Author(s):  
Monika Široká ◽  
Caterina Franco ◽  
Zuzana Guľašová ◽  
Zdenka Hertelyová ◽  
Vladimíra Tomečková ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Antioxidant Enzymes ◽  
Nitric Oxide Synthase ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Nuclear Factor ◽  
Obese Patients ◽  
Omega 3 ◽  
Oxide Synthase ◽  
Nuclear Factor Kb

Emerging evidence suggests that red blood cells (RBCs) are involved in many functions essential for life. Nuclear factor-kB (NF-kB), nitric oxide synthases (inducible nitric oxide synthase -iNOS-, endothelial nitric oxide synthase -eNOS-) and interleukin-1β (-IL-1β-) are all proteins that have been identified in RBCs. In nucleated cells, such as white blood cells (WBCs), these proteins have well investigated roles, linked to stress and inflammation. It is not the same in erythrocytes, for this reason, we considered obese patients for studying the morphology of RBCs. We studied a possible correlation between their morphological changes and several protein expressions. Moreover, we compared the results about the aforementioned proteins and antioxidant markers with those obtained in WBCs from healthy and obese patients before and after omega-3 polyunsaturated fatty acid supplementation. This latter scientific point is important in order to determine whether there are differences in the expression of nucleated and anucleated cells. The morphology of RBCs changed in obese patients, but it is significantly restored after six weeks of supplementation. The expression of antioxidant enzymes changed in RBCs and WBCs in obesity but all proteins restore their positivity after supplementation. We found that: the presence of NF-kB, antioxidant enzymes and eNOS in healthy RBCs could indicate a role of these proteins as regulators of cellular metabolism; obese WBCs showed a higher NF-kB, iNOS and IL-1β positivity, whereas eNOS presence did not significantly change in these cells. We tried to explain the different positivity of NF-kB, proposing a dual role for this protein, as prolifespan and as proinflammatory processes, depending on examined cells. In conclusion, we have considered the literature that focuses on the omega-6/omega-3 ratio. The ratio changed from the past, especially in people whose diet is strongly westernized worsening the state of health of the patient and leading to an higher incidence of obesity. Our study hypothesizes that the supplementation could help to restore the correct ratio.

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