A method for determining the positions of polar hydrogens added to a protein structure that maximizes protein hydrogen bonding

1992 ◽  
Vol 12 (3) ◽  
pp. 266-277 ◽  
Author(s):  
Michael B. Bass ◽  
Derek F. Hopkins ◽  
W. Andrew N. Jaquysh ◽  
Rick L. Ornstein
Keyword(s):  
Hydrogen Bonding ◽  
Protein Structure

Topological analysis of hydrogen bonding in protein structure

1990 ◽  
Vol 188 (2) ◽  
pp. 361-365 ◽  
Author(s):  
Boryeu MAO ◽  
Kuo-Chen CHOU ◽  
Gerald M. MAGGIORA
Keyword(s):  
Hydrogen Bonding ◽  
Protein Structure ◽  
Topological Analysis

Structural and Dynamic Evidence for C−H···O Hydrogen Bonding in Lariat Ethers:  Implications for Protein Structure

2000 ◽  
Vol 122 (14) ◽  
pp. 3325-3335 ◽  
Author(s):  
Eric S. Meadows ◽  
Stephen L. De Wall ◽  
Leonard J. Barbour ◽  
Frank R. Fronczek ◽  
Min-Sook Kim ◽  
...  
Keyword(s):  
Hydrogen Bonding ◽  
Protein Structure ◽  
Lariat Ethers

Adsorption, desorption, and activity of glucose oxidase on selected clay species

1976 ◽  
Vol 22 (5) ◽  
pp. 684-693 ◽  
Author(s):  
H. W. Morgan ◽  
C. T. Corke
Keyword(s):  
Hydrogen Bonding ◽  
Protein Structure ◽  
Cation Exchange ◽  
Glucose Oxidase ◽  
Isoelectric Point ◽  
Active Form ◽  
Temperature Dependent ◽  
Specific Activities ◽  
Ph Dependent ◽  
Adsorption Desorption

The adsorption of the enzyme glucose oxidase (EC 1.1.3.4) to clays followed the pattern described for other proteins as being pH dependent. Maximum adsorption occurred at or below the isoelectric point of the enzyme. The amount of enzyme adsorbed to clay was influenced by the type of clay used, and also the saturating cations. Initially adsorbed enzyme showed low specific activities, and as amounts of enzyme adsorbed approached maximum saturation of clay, specific activities increased approaching that determined for free enzyme.The adsorption of glucose oxidase involved a temperature-independent cation-exchange mechanism, and enzyme adsorbed to surfaces of clay could be desorbed in active form by elevation of pH of suspending solution. This was followed by a slower temperature-dependent fixation, probably by hydrogen bonding, which resulted in protein being irreversibly adsorbed to clay surfaces.It is proposed that on adsorption of glucose oxidase to clay surfaces unravelling of the protein structure occurred, which allowed penetration of protein into the interlamellar spaces of montmorillonite. This proposal was based on the observed expansion of montmorillonite to 23 Å, and the decreases in amount of a second-protein lysozyme adsorbed with extended incubation times of glucose oxidase – clay complexes at pH 4.5.

scholarly journals Protein structure prediction: Do hydrogen bonding and water-mediated interactions suffice?

Methods ◽  
2010 ◽  
Vol 52 (1) ◽  
pp. 84-90 ◽  
Author(s):  
Vanessa Oklejas ◽  
Chenghang Zong ◽  
Garegin A. Papoian ◽  
Peter G. Wolynes
Keyword(s):  
Hydrogen Bonding ◽  
Protein Structure ◽  
Protein Structure Prediction ◽  
Structure Prediction
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