scholarly journals Scaling‐up a fragment‐based protein‐protein interaction method using a human reference interaction set

2021 ◽  
Author(s):  
Stephanie Schaefer‐Ramadan ◽  
Jovana Aleksic ◽  
Nayra M. Al‐Thani ◽  
Yasmin A. Mohamoud ◽  
David E. Hill ◽  
...  
Keyword(s):  
Protein Interaction ◽  
Scaling Up ◽  
Interaction Method ◽  
Protein Protein Interaction

scholarly journals A Cell-Based Protein-Protein Interaction Method Using a Permuted Luciferase Reporter

2011 ◽  
Vol 5 ◽  
pp. 122-128 ◽  
Author(s):  
Haifeng Eishingdrelo ◽  
Jidong Cai ◽  
Paul Weissensee ◽  
Praveen Sharma ◽  
Michael J Tocci ◽  
...  
Keyword(s):  
Protein Interaction ◽  
Luciferase Reporter ◽  
Interaction Method ◽  
Protein Protein Interaction ◽  
A Cell

An efficient transient assays system using Agrobacterium-mediated transformation of onion (Allium cepa) epidermal cells

2020 ◽  
Vol 80 (03) ◽  
Author(s):  
Yu-Miao Zhang ◽  
Jun Wang ◽  
Tao Wu
Keyword(s):  
Subcellular Localization ◽  
Protein Interaction ◽  
Protein Interactions ◽  
Epidermal Cells ◽  
Cyclin Dependent Kinase ◽  
Protein Subcellular Localization ◽  
Protein Protein Interactions ◽  
Efficient System ◽  
Protein Protein Interaction ◽  
Onion Epidermal Cells

In this study, the Agrobacterium infection medium, infection duration, detergent, and cell density were optimized. The sorghum-based infection medium (SbIM), 10-20 min infection time, addition of 0.01% Silwet L-77, and Agrobacterium optical density at 600 nm (OD600), improved the competence of onion epidermal cells to support Agrobacterium infection at >90% efficiency. Cyclin-dependent kinase D-2 (CDKD-2) and cytochrome c-type biogenesis protein (CYCH), protein-protein interactions were localized. The optimized procedure is a quick and efficient system for examining protein subcellular localization and protein-protein interaction.

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