scholarly journals An NMR and MD study of complexes of bacteriophage lambda lysozyme with tetra‐ and hexa‐N‐acetylchitohexaose

2019 ◽  
Vol 88 (1) ◽  
pp. 82-93 ◽  
Author(s):  
Aysegul Turupcu ◽  
Alice M. Bowen ◽  
Alexandre Di Paolo ◽  
André Matagne ◽  
Chris Oostenbrink ◽  
...  
Keyword(s):  
1987 ◽  
Vol 262 (36) ◽  
pp. 17651-17658
Author(s):  
D A Steege ◽  
K C Cone ◽  
C Queen ◽  
M Rosenberg

1993 ◽  
Vol 268 (12) ◽  
pp. 8943-8948
Author(s):  
P.A. Hershberger ◽  
B.C. Mita ◽  
A. Tripatara ◽  
P.L. deHaseth

Genetics ◽  
1983 ◽  
Vol 104 (4) ◽  
pp. 549-570
Author(s):  
Ichizo Kobayashi ◽  
Mary M Stahl ◽  
David Leach ◽  
Franklin W Stahl

ABSTRACT Chi (5′-GCTGGTGG) is a recombinator in RecA- RecBC-mediated recombination in Escherichia coli. In bacteriophage λ vegetative recombination, Chi is fully active only when it is correctly oriented with respect to cos, the site that defines the ends of the packaged chromosome. Here we demonstrate that packaging from cos is not necessary for this cos-Chi interaction. Our evidence suggests that correctly oriented cos is an activator of Chi. cos, as an activator, is (1) dominant over cos  -, (2) active opposite an extensive heterology, (3) able to interact with Chi only when on the same (cis) chromosome, and (4) able to interact with Chi at distances as far as ≥ 20 kb. Thus, cos and Chi form a two-component recombinator system for general recombination. cos may serve as an asymmetric entry site for a recombination enzyme that recognizes Chi in an asymmetric way.


Gene ◽  
1979 ◽  
Vol 8 (1) ◽  
pp. 107-119 ◽  
Author(s):  
N.C. Franklin ◽  
G.N. Bennett

Genetics ◽  
1989 ◽  
Vol 121 (3) ◽  
pp. 401-409
Author(s):  
P Guzmán ◽  
G Guarneros

Abstract The rap mutation of Escherichia coli prevents the growth of bacteriophage lambda. We have isolated phage mutants that compensate for the host deficiency. The mutations, named bar, were genetically located to three different loci of the lambda genome: barI in the attP site, barII in the cIII ea10 region, and barIII within or very near the imm434 region. The level of lambda leftward transcription correlates with rap exclusion. Phage lambda mutants partially defective in the pL promoter or in pL-transcript antitermination showed a Bar- phenotype. Conversely, mutants constitutive for transcription from the pI or pL promoters were excluded more stringently by rap bacteria. We conclude that rap exclusion depends on the magnitude of transcription through the wild type bar loci in the phage genome.


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