Structure of a glycoside hydrolase family 50 enzyme from a subfamily that is enriched in human gut microbiome bacteroidetes

2016 ◽  
Vol 85 (1) ◽  
pp. 182-187 ◽  
Author(s):  
Kaleigh Giles ◽  
Benjamin Pluvinage ◽  
Alisdair B. Boraston
Keyword(s):  
Gut Microbiome ◽  
Glycoside Hydrolase ◽  
Glycoside Hydrolase Family ◽  
Human Gut ◽  
Human Gut Microbiome ◽  
Hydrolase Family

scholarly journals Discovery of α-l-arabinopyranosidases from human gut microbiome expands the diversity within glycoside hydrolase family 42

2017 ◽  
Vol 292 (51) ◽  
pp. 21092-21101 ◽  
Author(s):  
Alexander Holm Viborg ◽  
Takane Katayama ◽  
Takatoshi Arakawa ◽  
Maher Abou Hachem ◽  
Leila Lo Leggio ◽  
...  
Keyword(s):  
Gut Microbiome ◽  
Glycoside Hydrolase ◽  
Glycoside Hydrolase Family ◽  
Human Gut ◽  
Human Gut Microbiome ◽  
Hydrolase Family

scholarly journals Backbone 1H, 13C, and 15N resonance assignments of BoMan26A, a β-mannanase of the glycoside hydrolase family 26 from the human gut bacterium Bacteroides ovatus

2019 ◽  
Vol 13 (1) ◽  
pp. 213-218 ◽  
Author(s):  
Sven Wernersson ◽  
Viktoria Bågenholm ◽  
Cecilia Persson ◽  
Santosh Kumar Upadhyay ◽  
Henrik Stålbrand ◽  
...  
Keyword(s):  
Glycoside Hydrolase ◽  
Resonance Assignments ◽  
Glycoside Hydrolase Family ◽  
Human Gut ◽  
Bacteroides Ovatus ◽  
Hydrolase Family

scholarly journals Structure and function of Bs164 β-mannosidase from Bacteroides salyersiae the founding member of glycoside hydrolase family GH164

2019 ◽  
Vol 295 (13) ◽  
pp. 4316-4326 ◽  
Author(s):  
Zachary Armstrong ◽  
Gideon J. Davies
Keyword(s):  
Glycoside Hydrolase ◽  
Biochemical Characterization ◽  
Glycoside Hydrolase Family ◽  
Human Gut ◽  
X Ray Crystallography ◽  
Founding Member ◽  
And Function ◽  
Protein Sequence Space ◽  
Kinetics Of ◽  
Hydrolase Family

Recent work exploring protein sequence space has revealed a new glycoside hydrolase (GH) family (GH164) of putative mannosidases. GH164 genes are present in several commensal bacteria, implicating these genes in the degradation of dietary glycans. However, little is known about the structure, mechanism of action, and substrate specificity of these enzymes. Herein we report the biochemical characterization and crystal structures of the founding member of this family (Bs164) from the human gut symbiont Bacteroides salyersiae. Previous reports of this enzyme indicated that it has α-mannosidase activity, however, we conclusively show that it cleaves only β-mannose linkages. Using NMR spectroscopy, detailed enzyme kinetics of WT and mutant Bs164, and multiangle light scattering we found that it is a trimeric retaining β-mannosidase, that is susceptible to several known mannosidase inhibitors. X-ray crystallography revealed the structure of Bs164, the first known structure of a GH164, at 1.91 Å resolution. Bs164 is composed of three domains: a (β/α)8 barrel, a trimerization domain, and a β-sandwich domain, representing a previously unobserved structural-fold for β-mannosidases. Structures of Bs164 at 1.80–2.55 Å resolution in complex with the inhibitors noeuromycin, mannoimidazole, or 2,4-dinitrophenol 2-deoxy-2-fluoro-mannoside reveal the residues essential for specificity and catalysis including the catalytic nucleophile (Glu-297) and acid/base residue (Glu-160). These findings further our knowledge of the mechanisms commensal microbes use for nutrient acquisition.

scholarly journals Adaptation of Gut Microbiome to Transgenic Pigs Secreting β-Glucanase, Xylanase, and Phytase

2021 ◽  
Vol 12 ◽  
Author(s):  
Jianxin Mo ◽  
Guoling Li ◽  
Guangyan Huang ◽  
Haoqiang Wang ◽  
Junsong Shi ◽  
...  
Keyword(s):  
Gut Microbiome ◽  
Glycoside Hydrolase ◽  
Lactobacillus Reuteri ◽  
Nutrient Loss ◽  
Regulatory Subunit ◽  
Glycoside Hydrolase Family ◽  
Transgenic Pigs ◽  
Intestinal Microbes ◽  
Type D ◽  
Hydrolase Family

We previously generated transgenic pigs with enhanced growth rate and reduced nutrient loss. However, the composition of their gut microbiome is unknown. In this study, we successfully generated EGFP marker-free transgenic (MF-TG) pigs with high expression levels of microbial β-glucanase, xylanase, and phytase in the parotid gland. We collected intestinal contents from the ileum, cecum and colon of five MF-TG and five wild-type (WT) sows and investigated the gut microbiome of the transgenic pigs via metagenomic analysis. Results showed that the levels of probiotics, such as Lactobacillus reuteri and Streptococcus, were more abundant in the cecum of the MF-TG pigs and higher than those of WT pigs. By contrast, the levels of harmful microorganisms, such as Campylobacter, Chlamydia trachomatis, and Campylobacter fetus, and various unidentified viruses, were higher in the cecum of the WT pigs than those of the MF-TG pigs. By comparing unigenes and the eggNOG database, we found that the microorganisms in the colon of the MF-TG pigs had high fractional abundance in DNA (cytosine-5)-methyltransferase 1 and serine-type D-Ala-D-Ala carboxypeptidase, whereas the aspartate carbamoyltransferase regulatory subunit and outer membrane protein pathways were enriched in the WT pigs. Moreover, the microorganisms in the cecum of the MF-TG pigs were active in GlycosylTransferase Family 8 (GT8), Glycoside Hydrolase Family 13 (GH13), and Glycoside Hydrolase Family 32 (GH32). Furthermore, the levels of numerous carbohydrases, such as glucan 1,3-beta-glucosidase, xylan 1,4-beta-xylosidase and exo-1,3-1,4-glucanase, were higher in the cecum of the MF-TG pigs than those of the WT pigs. The results indicated that intestinal microbes can change adaptively to the secretion of transgenic enzymes, thereby forming a benign cooperation with their host. This cooperation could be beneficial for improving feed efficiency.

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