Role of active-site residues Tyr55 and Tyr114 in catalysis and substrate specificity ofCorynebacterium diphtheriaeC-S lyase

2014 ◽  
Vol 83 (1) ◽  
pp. 78-90 ◽  
Author(s):  
Alessandra Astegno ◽  
Alessandra Allegrini ◽  
Stefano Piccoli ◽  
Alejandro Giorgetti ◽  
Paola Dominici
Keyword(s):  
Substrate Specificity ◽  
Active Site ◽  
Active Site Residues

scholarly journals Porphyrin Binding and Distortion and Substrate Specificity in the Ferrochelatase Reaction: The Role of Active Site Residues

2008 ◽  
Vol 378 (5) ◽  
pp. 1074-1083 ◽  
Author(s):  
Tobias Karlberg ◽  
Mattias D. Hansson ◽  
Raymond K. Yengo ◽  
Renzo Johansson ◽  
Hege O. Thorvaldsen ◽  
...  
Keyword(s):  
Substrate Specificity ◽  
Active Site ◽  
Active Site Residues

Investigating the role of active site residues of Rhodotorula gracilis d-amino acid oxidase on its substrate specificity

Biochimie ◽  
2007 ◽  
Vol 89 (3) ◽  
pp. 360-368 ◽  
Author(s):  
Angelo Boselli ◽  
Luciano Piubelli ◽  
Gianluca Molla ◽  
Mirella S. Pilone ◽  
Loredano Pollegioni ◽  
...  
Keyword(s):  
Amino Acid ◽  
Substrate Specificity ◽  
Active Site ◽  
Acid Oxidase ◽  
Active Site Residues ◽  
Amino Acid Oxidase ◽  
Rhodotorula Gracilis

scholarly journals Probing the role of the residues in the active site of the transaminase from Thermobaculum terrenum

PLoS ONE ◽  
2021 ◽  
Vol 16 (7) ◽  
pp. e0255098
Author(s):  
Ekaterina Yu. Bezsudnova ◽  
Alena Yu. Nikolaeva ◽  
Alina K. Bakunova ◽  
Tatiana V. Rakitina ◽  
Dmitry A. Suplatov ◽  
...  
Keyword(s):  
Amino Acids ◽  
Substrate Specificity ◽  
Active Site ◽  
Active Sites ◽  
Site Directed Mutagenesis ◽  
Primary Amines ◽  
Active Site Residues ◽  
Type Iv ◽  
Fold Type

Creating biocatalysts for (R)-selective amination effectively is highly desirable in organic synthesis. Despite noticeable progress in the engineering of (R)-amine activity in pyridoxal-5’-phosphate-dependent transaminases of fold type IV, the specialization of the activity is still an intuitive task, as there is poor understanding of sequence-structure-function relationships. In this study, we analyzed this relationship in transaminase from Thermobaculum terrenum, distinguished by expanded substrate specificity and activity in reactions with L-amino acids and (R)-(+)-1-phenylethylamine using α-ketoglutarate and pyruvate as amino acceptors. We performed site-directed mutagenesis to create a panel of the enzyme variants, which differ in the active site residues from the parent enzyme to a putative transaminase specific to (R)-primary amines. The variants were examined in the overall transamination reactions and half-reaction with (R)-(+)-1-phenylethylamine. A structural analysis of the most prominent variants revealed a spatial reorganization in the active sites, which caused changes in activity. Although the specialization to (R)-amine transaminase was not implemented, we succeeded in understanding the role of the particular active site residues in expanding substrate specificity of the enzyme. We showed that the specificity for (R)-(+)-1-phenylethylamine in transaminase from T. terrenum arises without sacrificing the specificity for L-amino acids and α-ketoglutarate and in consensus with it.

Role of the active site residues arginine-216 and arginine-237 in the substrate specificity of mammalian d-aspartate oxidase

Amino Acids ◽  
2010 ◽  
Vol 40 (2) ◽  
pp. 467-476 ◽  
Author(s):  
Masumi Katane ◽  
Yasuaki Saitoh ◽  
Kazuhiro Maeda ◽  
Toshihiko Hanai ◽  
Masae Sekine ◽  
...  
Keyword(s):  
Substrate Specificity ◽  
Active Site ◽  
Active Site Residues

scholarly journals Redesigning the Substrate Specificity of an Enzyme by Cumulative Effects of the Mutations of Non-active Site Residues

1999 ◽  
Vol 274 (4) ◽  
pp. 2344-2349 ◽  
Author(s):  
Shinya Oue ◽  
Akihiro Okamoto ◽  
Takato Yano ◽  
Hiroyuki Kagamiyama
Keyword(s):  
Substrate Specificity ◽  
Active Site ◽  
Cumulative Effects ◽  
Active Site Residues

scholarly journals Mechanism of the Flavoprotein d-6-Hydroxynicotine Oxidase: Substrate Specificity, pH and Solvent Isotope Effects, and Roles of Key Active-Site Residues

Biochemistry ◽  
2019 ◽  
Vol 58 (21) ◽  
pp. 2534-2541
Author(s):  
Paul F. Fitzpatrick ◽  
Vi Dougherty ◽  
Bishnu Subedi ◽  
Jesus Quilantan ◽  
Cynthia S. Hinck ◽  
...  
Keyword(s):  
Substrate Specificity ◽  
Active Site ◽  
Isotope Effects ◽  
Active Site Residues ◽  
Solvent Isotope ◽  
Solvent Isotope Effects
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