NMR structure and dynamics of recombinant wild type and mutated jerdostatin, a selective inhibitor of integrin α1 β1

2011 ◽  
Vol 79 (8) ◽  
pp. 2530-2542 ◽  
Author(s):  
Rodrigo J. Carbajo ◽  
Libia Sanz ◽  
Silvia Mosulén ◽  
Alicia Pérez ◽  
Cezary Marcinkiewicz ◽  
...  
Keyword(s):  
Selective Inhibitor ◽  
Nmr Structure ◽  
Wild Type ◽  
Structure And Dynamics

scholarly journals Catecholamine Surges Cause Cardiomyocyte Necroptosis via a RIPK1–RIPK3-Dependent Pathway in Mice

2021 ◽  
Vol 8 ◽  
Author(s):  
Penglong Wu ◽  
Mingqi Cai ◽  
Jinbao Liu ◽  
Xuejun Wang
Keyword(s):  
Heart Failure ◽  
Cardiac Injury ◽  
Underlying Mechanism ◽  
Selective Inhibitor ◽  
Blue Dye ◽  
Wild Type ◽  
Adrenergic Stimulation ◽  
Protein Levels ◽  
Regulated Necrosis ◽  
Dependent Pathway

Background: Catecholamine surges and resultant excessive β-adrenergic stimulation occur in a broad spectrum of diseases. Excessive β-adrenergic stimulation causes cardiomyocyte necrosis, but the underlying mechanism remains obscure. Necroptosis, a major form of regulated necrosis mediated by RIPK3-centered pathways, is implicated in heart failure; however, it remains unknown whether excessive β-adrenergic stimulation-induced cardiac injury involves necroptosis. Hence, we conducted the present study to address these critical gaps.Methods and Results: Two consecutive daily injections of isoproterenol (ISO; 85 mg/kg, s.c.) or saline were administered to adult mixed-sex mice. At 24 h after the second ISO injection, cardiac area with Evans blue dye (EBD) uptake and myocardial protein levels of CD45, RIPK1, Ser166-phosphorylated RIPK1, RIPK3, and Ser345-phosphorylated MLKL (p-MLKL) were significantly greater, while Ser321-phosphorylated RIPK1 was significantly lower, in the ISO-treated than in saline-treated wild-type (WT) mice. The ISO-induced increase of EBD uptake was markedly less in RIPK3−/− mice compared with WT mice (p = 0.016). Pretreatment with the RIPK1-selective inhibitor necrostatin-1 diminished ISO-induced increases in RIPK3 and p-MLKL in WT mice and significantly attenuated ISO-induced increases of EBD uptake in WT but not RIPK3−/− mice.Conclusions: A large proportion of cardiomyocyte necrosis induced by excessive β-adrenergic stimulation belongs to necroptosis and is mediated by a RIPK1–RIPK3-dependent pathway, identifying RIPK1 and RIPK3 as potential therapeutic targets for catecholamine surges.

scholarly journals NMR Structure and Dynamics Studies of Yeast Respiratory Supercomplex Factor 2

Structure ◽  
2020 ◽  
Author(s):  
Shu Zhou ◽  
Pontus Pettersson ◽  
Jingjing Huang ◽  
Peter Brzezinski ◽  
Régis Pomès ◽  
...  
Keyword(s):  
Nmr Structure ◽  
Structure And Dynamics

scholarly journals NMR structure and dynamics of the RNA-binding site for the histone mRNA stem-loop binding protein

RNA ◽  
2002 ◽  
Vol 8 (1) ◽  
pp. 83-96 ◽  
Author(s):  
ERIC S. DEJONG ◽  
WILLIAM F. MARZLUFF ◽  
EDWARD P. NIKONOWICZ
Keyword(s):  
Binding Site ◽  
Rna Binding ◽  
Binding Protein ◽  
Nmr Structure ◽  
Histone Mrna ◽  
Stem Loop ◽  
Structure And Dynamics

Structure and Dynamics of the Glucocorticoid Receptor DNA-Binding Domain:  Comparison of Wild Type and a Mutant with Altered Specificity†

Biochemistry ◽  
1997 ◽  
Vol 36 (37) ◽  
pp. 11188-11197 ◽  
Author(s):  
Helena Berglund ◽  
Magnus Wolf-Watz ◽  
Thomas Lundbäck ◽  
Susanne van den Berg ◽  
Torleif Härd
Keyword(s):  
Dna Binding ◽  
Glucocorticoid Receptor ◽  
Binding Domain ◽  
Dna Binding Domain ◽  
Wild Type ◽  
Structure And Dynamics

scholarly journals NMR Structure and Dynamics of a Receptor-active Apolipoprotein E Peptide

2002 ◽  
Vol 277 (32) ◽  
pp. 29172-29180 ◽  
Author(s):  
Vincent Raussens ◽  
Carolyn M. Slupsky ◽  
Robert O. Ryan ◽  
Brian D. Sykes
Keyword(s):  
Apolipoprotein E ◽  
Nmr Structure ◽  
Structure And Dynamics
Sign in / Sign up

Export Citation Format

Share Document