Dissecting the molecular mechanism of drosophila odorant receptors through activity modeling and comparative analysis

2010 ◽  
Vol 78 (2) ◽  
pp. 381-399 ◽  
Author(s):  
Sheng Guo ◽  
Junhyong Kim
Keyword(s):  
Comparative Analysis ◽  
Molecular Mechanism ◽  
Odorant Receptors ◽  
Activity Modeling

scholarly journals Comparative analysis of molecular mechanism of spinal cord injury with time based on bioinformatics data

Spinal Cord ◽  
2015 ◽  
Vol 54 (6) ◽  
pp. 431-438 ◽  
Author(s):  
T Wen ◽  
J Hou ◽  
F Wang ◽  
Y Zhang ◽  
T Zhang ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Comparative Analysis ◽  
Molecular Mechanism ◽  
Cord Injury

scholarly journals Comparative analysis of the molecular mechanism of resistance to vapendavir across a panel of picornavirus species

2021 ◽  
Vol 195 ◽  
pp. 105177
Author(s):  
Kristina Lanko ◽  
Liang Sun ◽  
Mathy Froeyen ◽  
Pieter Leyssen ◽  
Leen Delang ◽  
...  
Keyword(s):  
Comparative Analysis ◽  
Molecular Mechanism ◽  
Mechanism Of Resistance

scholarly journals Comparative analysis of the molecular mechanism of resistance to vapendavir across a panel of picornavirus species

2020 ◽  
Author(s):  
Kristina Lanko ◽  
Liang Sun ◽  
Mathy Froeyen ◽  
Pieter Leyssen ◽  
Leen Delang ◽  
...  
Keyword(s):  
Comparative Analysis ◽  
Molecular Mechanism ◽  
Viral Particle ◽  
Binding Pocket ◽  
Drug Binding ◽  
Viral Capsid ◽  
Drug Resistant ◽  
Hydrophobic Pocket ◽  
Resistant Phenotype

AbstractVapendavir is a rhino/enterovirus inhibitor that targets a hydrophobic pocket in the viral capsid. Drug-resistant variants were selected in vitro. Mutations in the drug-binding pocket in VP1 (C199R/Y in hRV14; I194F in PV1; M252L and A156T in EV-D68), typical for this class of compounds, were identified. We also observed mutations that are located outside the pocket (K167E in EV-D68 and G149C in hRV2) and that contribute to the resistant phenotype. Remarkably, the G149C substitution made the replication of human rhinovirus 2 dependent on the presence of vapendavir. Our data suggest that vapendavir binding to the capsid of the dependent isolate may be required to stabilize the viral particle and to allow efficient dissemination of the virus. Our results demonstrate that vapendavir-resistant pheno- and genotypes of clinically relevant picornavirus species are more complex than generally believed.

scholarly journals Odorant Receptors for Detecting Flowering Plant Cues Are Functionally Conserved across Moths and Butterflies

2020 ◽  
Author(s):  
Mengbo Guo ◽  
Lixiao Du ◽  
Qiuyan Chen ◽  
Yilu Feng ◽  
Jin Zhang ◽  
...  
Keyword(s):  
Comparative Analysis ◽  
Large Scale ◽  
Floral Scent ◽  
Flowering Plant ◽  
Odorant Receptors ◽  
In Vivo Studies ◽  
Systematic Analysis ◽  
Plant Volatile ◽  
Nectar Feeding

Abstract Odorant receptors (ORs) are essential for plant–insect interactions. However, despite the global impacts of Lepidoptera (moths and butterflies) as major herbivores and pollinators, little functional data are available about Lepidoptera ORs involved in plant-volatile detection. Here, we initially characterized the plant-volatile-sensing function(s) of 44 ORs from the cotton bollworm Helicoverpa armigera, and subsequently conducted a large-scale comparative analysis that establishes how most orthologous ORs have functionally diverged among closely related species whereas some rare ORs are functionally conserved. Specifically, our systematic analysis of H. armigera ORs cataloged the wide functional scope of the H. armigera OR repertoire, and also showed that HarmOR42 and its Spodoptera littoralis ortholog are functionally conserved. Pursuing this, we characterized the HarmOR42-orthologous ORs from 11 species across the Glossata suborder and confirmed the HarmOR42 orthologs form a unique OR lineage that has undergone strong purifying selection in Glossata species and whose members are tuned with strong specificity to phenylacetaldehyde, a floral scent component common to most angiosperms. In vivo studies via HarmOR42 knockout support that HarmOR42-related ORs are essential for host-detection by sensing phenylacetaldehyde. Our work also supports that these ORs coevolved with the tube-like proboscis, and has maintained functional stability throughout the long-term coexistence of Lepidoptera with angiosperms. Thus, beyond providing a rich empirical resource for delineating the precise functions of H. armigera ORs, our results enable a comparative analysis of insect ORs that have apparently facilitated and currently sustain the intimate adaptations and ecological interactions among nectar feeding insects and flowering plants.

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