Structural determinants of ligand migration in Mycobacterium tuberculosis truncated hemoglobin O

2008 ◽  
Vol 73 (2) ◽  
pp. 372-379 ◽  
Author(s):  
Leonardo Boechi ◽  
Marcelo A. Martí ◽  
Mario Milani ◽  
Martino Bolognesi ◽  
F. Javier Luque ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Truncated Hemoglobin ◽  
Structural Determinants ◽  
Ligand Migration

scholarly journals Ligand uptake in Mycobacterium tuberculosis truncated hemoglobins is controlled by both internal tunnels and active site water molecules

F1000Research ◽  
2015 ◽  
Vol 4 ◽  
pp. 22 ◽  
Author(s):  
Ignacio Boron ◽  
Juan Pablo Bustamante ◽  
Kelly S Davidge ◽  
Sandip Singh ◽  
Lesley AH Bowman ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Active Site ◽  
Water Molecules ◽  
Single Mutation ◽  
Truncated Hemoglobin ◽  
Kinetic Measurements ◽  
Migration Rates ◽  
Ligand Migration ◽  
Site Water ◽  
Dynamics Simulations

Mycobacterium tuberculosis, the causative agent of human tuberculosis, has two proteins belonging to the truncated hemoglobin (trHb) family. Mt-trHbN presents well-defined internal hydrophobic tunnels that allow O2 and •NO to migrate easily from the solvent to the active site, whereas Mt-trHbO possesses tunnels that are partially blocked by a few bulky residues, particularly a tryptophan at position G8. Differential ligand migration rates allow Mt-trHbN to detoxify •NO, a crucial step for pathogen survival once under attack by the immune system, much more efficiently than Mt-trHbO. In order to investigate the differences between these proteins, we performed experimental kinetic measurements, •NO decomposition, as well as molecular dynamics simulations of the wild type Mt-trHbN and two mutants, VG8F and VG8W. These mutations introduce modifications in both tunnel topologies and affect the incoming ligand capacity to displace retained water molecules at the active site. We found that a single mutation allows Mt-trHbN to acquire ligand migration rates comparable to those observed for Mt-trHbO, confirming that ligand migration is regulated by the internal tunnel architecture as well as by water molecules stabilized in the active site.

scholarly journals Ligand migration in the truncated hemoglobin of Mycobacterium tuberculosis

IUBMB Life ◽  
2011 ◽  
Vol 63 (3) ◽  
pp. 214-220 ◽  
Author(s):  
Maxime S. Heroux ◽  
Anne D. Mohan ◽  
Kenneth W. Olsen
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Truncated Hemoglobin ◽  
Ligand Migration

scholarly journals Ligand uptake in Mycobacterium tuberculosis truncated hemoglobins is controlled by both internal tunnels and active site water molecules

F1000Research ◽  
2015 ◽  
Vol 4 ◽  
pp. 22 ◽  
Author(s):  
Ignacio Boron ◽  
Juan Pablo Bustamante ◽  
Kelly S Davidge ◽  
Sandip Singh ◽  
Lesley AH Bowman ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Active Site ◽  
Water Molecules ◽  
Single Mutation ◽  
Truncated Hemoglobin ◽  
Kinetic Measurements ◽  
Migration Rates ◽  
Ligand Migration ◽  
Site Water ◽  
Dynamics Simulations

Mycobacterium tuberculosis, the causative agent of human tuberculosis, has two proteins belonging to the truncated hemoglobin (trHb) family. Mt-trHbN presents well-defined internal hydrophobic tunnels that allow O2 and •NO to migrate easily from the solvent to the active site, whereas Mt-trHbO possesses tunnels interrupted by a few bulky residues, particularly a tryptophan at position G8. Differential ligand migration rates allow Mt-trHbN to detoxify •NO, a crucial step for pathogen survival once under attack by the immune system, much more efficiently than Mt-trHbO. In order to investigate the differences between these proteins, we performed experimental kinetic measurements, •NO decomposition, as well as molecular dynamics simulations of the wild type Mt-trHbN and two mutants, VG8F and VG8W. These mutations affect both the tunnels accessibility as well as the affinity of distal site water molecules, thus modifying the ligand access to the iron. We found that a single mutation allows Mt-trHbN to acquire ligand migration rates comparable to those observed for Mt-trHbO, confirming that ligand migration is regulated by the internal tunnel architecture as well as by water molecules stabilized in the active site.

scholarly journals Following Ligand Migration Pathways from Picoseconds to Milliseconds in Type II Truncated Hemoglobin from Thermobifida fusca

PLoS ONE ◽  
2012 ◽  
Vol 7 (7) ◽  
pp. e39884 ◽  
Author(s):  
Agnese Marcelli ◽  
Stefania Abbruzzetti ◽  
Juan Pablo Bustamante ◽  
Alessandro Feis ◽  
Alessandra Bonamore ◽  
...  
Keyword(s):  
Thermobifida Fusca ◽  
Type Ii ◽  
Truncated Hemoglobin ◽  
Ligand Migration ◽  
Migration Pathways

scholarly journals Theoretical Investigations of Nitric Oxide Channeling in Mycobacterium tuberculosis Truncated Hemoglobin N

2009 ◽  
Vol 97 (11) ◽  
pp. 2967-2977 ◽  
Author(s):  
Richard Daigle ◽  
Julie-Anne Rousseau ◽  
Michel Guertin ◽  
Patrick Lagüe
Keyword(s):  
Nitric Oxide ◽  
Mycobacterium Tuberculosis ◽  
Truncated Hemoglobin ◽  
Theoretical Investigations

scholarly journals Role of PheE15 Gate in Ligand Entry and Nitric Oxide Detoxification Function of Mycobacterium tuberculosis Truncated Hemoglobin N

PLoS ONE ◽  
2012 ◽  
Vol 7 (11) ◽  
pp. e49291 ◽  
Author(s):  
Ana Oliveira ◽  
Sandeep Singh ◽  
Axel Bidon-Chanal ◽  
Flavio Forti ◽  
Marcelo A. Martí ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Mycobacterium Tuberculosis ◽  
Truncated Hemoglobin ◽  
Nitric Oxide Detoxification

scholarly journals Structural Determinants in the Group III Truncated Hemoglobin fromCampylobacter jejuni

2006 ◽  
Vol 281 (49) ◽  
pp. 37803-37812 ◽  
Author(s):  
Marco Nardini ◽  
Alessandra Pesce ◽  
Marie Labarre ◽  
Christian Richard ◽  
Alessandro Bolli ◽  
...  
Keyword(s):  
Truncated Hemoglobin ◽  
Structural Determinants ◽  
Group Iii

Dynamical Regulation of Ligand Migration by a Gate-Opening Molecular Switch in Truncated Hemoglobin-N fromMycobacterium tuberculosis

2007 ◽  
Vol 129 (21) ◽  
pp. 6782-6788 ◽  
Author(s):  
Axel Bidon-Chanal ◽  
Marcelo A. Martí ◽  
Darío A. Estrin ◽  
F. Javier Luque
Keyword(s):  
Molecular Switch ◽  
Truncated Hemoglobin ◽  
Gate Opening ◽  
Ligand Migration

scholarly journals Isoniazid Inhibits the Heme-Based Reactivity of Mycobacterium tuberculosis Truncated Hemoglobin N

PLoS ONE ◽  
2013 ◽  
Vol 8 (8) ◽  
pp. e69762 ◽  
Author(s):  
Paolo Ascenzi ◽  
Andrea Coletta ◽  
Yu Cao ◽  
Viviana Trezza ◽  
Loris Leboffe ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Truncated Hemoglobin

Ultrafast heme–ligand recombination in truncated hemoglobin HbO from Mycobacterium tuberculosis: A ligand cage

2012 ◽  
Vol 396 ◽  
pp. 10-16 ◽  
Author(s):  
Audrius Jasaitis ◽  
Hugues Ouellet ◽  
Jean-Christophe Lambry ◽  
Jean-Louis Martin ◽  
Joel M. Friedman ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Truncated Hemoglobin
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