scholarly journals Crystal structure of VC1805, a conserved hypothetical protein from a Vibrio cholerae pathogenicity island, reveals homology to human p32

2008 ◽  
Vol 71 (3) ◽  
pp. 1563-1571 ◽  
Author(s):  
Md. Arif Sheikh ◽  
Jane A. Potter ◽  
Kenneth A. Johnson ◽  
Robert B. Sim ◽  
E. Fidelma Boyd ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Vibrio Cholerae ◽  
Pathogenicity Island ◽  
Hypothetical Protein

Crystal structure of VC0702 at 2.0 Å: Conserved hypothetical protein from Vibrio cholerae

2006 ◽  
Vol 63 (4) ◽  
pp. 733-741 ◽  
Author(s):  
Shuisong Ni ◽  
Farhad Forouhar ◽  
Dirksen E. Bussiere ◽  
Howard Robinson ◽  
Michael A. Kennedy
Keyword(s):  
Crystal Structure ◽  
Vibrio Cholerae ◽  
Hypothetical Protein

scholarly journals Pathogenic Potential of Environmental Vibrio cholerae Strains Carrying Genetic Variants of the Toxin-Coregulated Pilus Pathogenicity Island

2003 ◽  
Vol 71 (2) ◽  
pp. 1020-1025 ◽  
Author(s):  
Shah M. Faruque ◽  
M. Kamruzzaman ◽  
Ismail M. Meraj ◽  
Nityananda Chowdhury ◽  
G. Balakrish Nair ◽  
...  
Keyword(s):  
Vibrio Cholerae ◽  
Genetic Variants ◽  
Pathogenicity Island ◽  
Biologically Active ◽  
Pathogenic Potential ◽  
Origin And Evolution ◽  
Colonization Factor ◽  
Evolution Of Virulence ◽  
Toxin Coregulated Pilus ◽  
El Tor

ABSTRACT The major virulence factors of toxigenic Vibrio cholerae are cholera toxin (CT), which is encoded by a lysogenic bacteriophage (CTXΦ), and toxin-coregulated pilus (TCP), an essential colonization factor which is also the receptor for CTXΦ. The genes for the biosynthesis of TCP are part of a larger genetic element known as the TCP pathogenicity island. To assess their pathogenic potential, we analyzed environmental strains of V. cholerae carrying genetic variants of the TCP pathogenicity island for colonization of infant mice, susceptibility to CTXΦ, and diarrheagenicity in adult rabbits. Analysis of 14 environmental strains, including 3 strains carrying a new allele of the tcpA gene, 9 strains carrying a new allele of the toxT gene, and 2 strains carrying conventional tcpA and toxT genes, showed that all strains colonized infant mice with various efficiencies in competition with a control El Tor biotype strain of V. cholerae O1. Five of the 14 strains were susceptible to CTXΦ, and these transductants produced CT and caused diarrhea in adult rabbits. These results suggested that the new alleles of the tcpA and toxT genes found in environmental strains of V. cholerae encode biologically active gene products. Detection of functional homologs of the TCP island genes in environmental strains may have implications for understanding the origin and evolution of virulence genes of V. cholerae.

scholarly journals Examination of Diverse Toxin-Coregulated Pilus-Positive Vibrio cholerae Strains Fails To Demonstrate Evidence for Vibrio Pathogenicity Island Phage

2003 ◽  
Vol 71 (6) ◽  
pp. 2993-2999 ◽  
Author(s):  
Shah M. Faruque ◽  
Jun Zhu ◽  
Asadulghani ◽  
M. Kamruzzaman ◽  
John J. Mekalanos
Keyword(s):  
Vibrio Cholerae ◽  
Virulence Factors ◽  
Genetic Variants ◽  
Horizontal Transfer ◽  
Pathogenicity Island ◽  
Culture Conditions ◽  
Filamentous Phage ◽  
Colonization Factor ◽  
Toxin Coregulated Pilus ◽  
El Tor

ABSTRACT The major virulence factors of toxigenic Vibrio cholerae are cholera toxin, which is encoded by a lysogenic filamentous bacteriophage (CTXΦ), and toxin-coregulated pilus (TCP), an essential colonization factor that is also the receptor for CTXΦ. The genes involved in the biosynthesis of TCP reside in a pathogenicity island, which has been reported to correspond to the genome of another filamentous phage (designated VPIΦ) and to encode functions necessary for the production of infectious VPIΦ particles. We examined 46 V. cholerae strains having diverse origins and carrying different genetic variants of the TCP island for the production of the VPIΦ and CTXΦ in different culture conditions, including induction of prophages with mitomycin C and UV irradiation. Although 9 of 10 V. cholerae O139 strains and 12 of 15 toxigenic El Tor strains tested produced extracellular CTXΦ, none of the 46 TCP-positive strains produced detectable VPIΦ in repeated assays, which detected as few as 10 particles of a control CTX phage per ml. These results contradict the previous report regarding VPIΦ-mediated horizontal transfer of the TCP genes and suggest that the TCP island is unable to support the production of phage particles. Further studies are necessary to understand the mechanism of horizontal transfer of the TCP island.

scholarly journals Comparative Genomic Analyses of the Vibrio Pathogenicity Island and Cholera Toxin Prophage Regions in Nonepidemic Serogroup Strains of Vibrio cholerae

2003 ◽  
Vol 69 (3) ◽  
pp. 1728-1738 ◽  
Author(s):  
Manrong Li ◽  
Mamuka Kotetishvili ◽  
Yuansha Chen ◽  
Shanmuga Sozhamannan
Keyword(s):  
Cholera Toxin ◽  
Vibrio Cholerae ◽  
Fragment Length Polymorphism ◽  
Pathogenicity Island ◽  
Filamentous Phage ◽  
Comparative Genomic ◽  
Genomic Analyses ◽  
Ctxφ Prophage ◽  
Horizontal Acquisition ◽  
Restriction Fragment Length

ABSTRACT Two major virulence factors are associated with epidemic strains (O1 and O139 serogroups) of Vibrio cholerae: cholera toxin encoded by the ctxAB genes and toxin-coregulated pilus encoded by the tcpA gene. The ctx genes reside in the genome of a filamentous phage (CTXφ), and the tcpA gene resides in a vibrio pathogenicity island (VPI) which has also been proposed to be a filamentous phage designated VPIφ. In order to determine the prevalence of horizontal transfer of VPI and CTXφ among nonepidemic (non-O1 and non-O139 serogroups) V. cholerae, 300 strains of both clinical and environmental origin were screened for the presence of tcpA and ctxAB. In this paper, we present the comparative genetic analyses of 11 nonepidemic serogroup strains which carry the VPI cluster. Seven of the 11 VPI+ strains have also acquired the CTXφ. Multilocus sequence typing and restriction fragment length polymorphism analyses of the VPI and CTXφ prophage regions revealed that the non-O1 and non-O139 strains were genetically diverse and clustered in lineages distinct from that of the epidemic strains. The left end of the VPI in the non-O1 and non-O139 strains exhibited extensive DNA rearrangements. In addition, several CTXφ prophage types characterized by novel repressor (rstR) and ctxAB genes and VPIs with novel tcpA genes were found in these strains. These data suggest that the potentially pathogenic, nonepidemic, non-O1 and non-O139 strains identified in our study most likely evolved by sequential horizontal acquisition of the VPI and CTXφ independently rather than by exchange of O-antigen biosynthesis regions in an existing epidemic strain.

Crystal structure and activity of protein L-isoaspartyl-O-methyltransferase from Vibrio cholerae, and the effect of AdoHcy binding

2015 ◽  
Vol 583 ◽  
pp. 140-149 ◽  
Author(s):  
Tanaya Chatterjee ◽  
Debadrita Mukherjee ◽  
Mousumi Banerjee ◽  
Barun K. Chatterjee ◽  
Pinak Chakrabarti
Keyword(s):  
Crystal Structure ◽  
Vibrio Cholerae ◽  
Protein L ◽  
Structure And Activity

Crystal structure of the hypothetical protein TTHA1013 from Thermus thermophilus HB8

2005 ◽  
Vol 61 (4) ◽  
pp. 1117-1120 ◽  
Author(s):  
Motoyuki Hattori ◽  
Eiichi Mizohata ◽  
Miho Manzoku ◽  
Yoshitaka Bessho ◽  
Kazutaka Murayama ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Thermus Thermophilus ◽  
Hypothetical Protein ◽  
Thermus Thermophilus Hb8

Crystal structure analysis of a hypothetical protein (MJ0366) from Methanocaldococcus jannaschii revealed a novel topological arrangement of the knot fold

2017 ◽  
Vol 482 (2) ◽  
pp. 264-269 ◽  
Author(s):  
Viswanathan Thiruselvam ◽  
Thirumananseri Kumarevel ◽  
Ponnuraj Karthe ◽  
Seiki Kuramitsu ◽  
Shigeyuki Yokoyama ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Structure Analysis ◽  
Hypothetical Protein ◽  
Crystal Structure Analysis ◽  
Methanocaldococcus Jannaschii

scholarly journals Crystal structure of hypothetical protein PH0828 fromPyrococcus horikoshii

2004 ◽  
Vol 57 (4) ◽  
pp. 862-865 ◽  
Author(s):  
Yosuke Tajika ◽  
Naoki Sakai ◽  
Tomohiro Tamura ◽  
Min Yao ◽  
Nobuhisa Watanabe ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Hypothetical Protein
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