Conformational stability and DNA binding energetics of the rat thyroid transcription factor 1 homeodomain

2008 ◽  
Vol 70 (3) ◽  
pp. 748-760 ◽  
Author(s):  
Pompea Del Vecchio ◽  
Paola Carullo ◽  
Guido Barone ◽  
Bruno Pagano ◽  
Giuseppe Graziano ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Dna Binding ◽  
Conformational Stability ◽  
Thyroid Transcription Factor 1 ◽  
Thyroid Transcription Factor ◽  
Binding Energetics ◽  
Rat Thyroid ◽  
Transcription Factor 1

Disrupted co-ordination of Pax-8 and thyroid transcription factor-1 gene expression in a dedifferentiated rat thyroid tumour cell line derived from FRTL-5

1996 ◽  
Vol 150 (3) ◽  
pp. 377-382 ◽  
Author(s):  
C J H van der Kallen ◽  
D C J Spierings ◽  
J H H Thijssen ◽  
M A Blankenstein ◽  
T W A de Bruin
Keyword(s):  
Gene Expression ◽  
Transcription Factor ◽  
Cell Line ◽  
Thyroid Peroxidase ◽  
Wild Type ◽  
Specific Expression ◽  
Thyroid Transcription Factor 1 ◽  
Thyroid Transcription Factor ◽  
Rat Thyroid ◽  
Transcription Factor 1

Abstract The mutant rat thyroid cell line FRTL-5/TA, isolated from a non-functional tumour which originated spontaneously from wild-type FRTL-5 cells, shows autonomous TSH-independent growth and loss of the thyroid-specific phenotype, lacking thyroid-specific expression of thyroglobulin (Tg) and thyroid peroxidase (TPO) genes. To investigate the role of the transcription factors Pax-8 and thyroid transcription factor-1 (TTF-1) in rat thyroid tumorigenesis, RNA expression of these two thyroid-specific nuclear factors was measured in FRTL-5/TA tumour cells and compared with the expression in wild-type FRTL-5 cells. TTF-1 gene expression was similar to that in wild-type FRTL-5, and showed a similar down-regulation after stimulation with TSH. The finding suggested normal TTF-1 mRNA and protein expression in both cell lines. By contrast, Pax-8 mRNA transcript signal was markedly reduced in FRTL-5/TA cells, reaching levels as low as 8% of the normal, basal level in FRTL-5 cells. These data indicated that the loss of thyroid-specific expression of Tg and TPO genes in FRTL-5/TA cells was not related to changes in TTF-1 gene expression but rather to reduced Pax-8 gene expression. It was concluded that a disruption of the co-ordinated expression of TTF-1 and Pax-8 is implicated in the loss of thyroid phenotype of FRTL-5/TA cells in terms of reduced Tg and TPO expression. Journal of Endocrinology (1996) 150, 377–382

scholarly journals Thyroid Transcription Factor-1 Activates the Promoter Activity of Rat Thyroid Na+/I−Symporter Gene

1997 ◽  
Vol 11 (11) ◽  
pp. 1747-1755 ◽  
Author(s):  
Toyoshi Endo ◽  
Masahiro Kaneshige ◽  
Minoru Nakazato ◽  
Masayuki Ohmori ◽  
Norikazu Harii ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Promoter Activity ◽  
Thyroid Transcription Factor 1 ◽  
Thyroid Transcription Factor ◽  
Rat Thyroid ◽  
Transcription Factor 1
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