The role of calcium in the conformational dynamics and thermal stability of the D-galactose/D-glucose-binding protein from Escherichia coli

2005 ◽  
Vol 61 (1) ◽  
pp. 184-195 ◽  
Author(s):  
Petr Herman ◽  
Jaroslav Vecer ◽  
Ivan Barvik ◽  
Viviana Scognamiglio ◽  
Maria Staiano ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Thermal Stability ◽  
Binding Protein ◽  
Conformational Dynamics ◽  
Glucose Binding ◽  
Glucose Binding Protein ◽  
Thermal Stability Of

Pressure effect on the stability and the conformational dynamics of the D-Galactose/D-Glucose-binding protein from Escherichia coli

2005 ◽  
Vol 62 (1) ◽  
pp. 193-201 ◽  
Author(s):  
Anna Marabotti ◽  
Petr Herman ◽  
Maria Staiano ◽  
Antonio Varriale ◽  
Marcella de Champdoré ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Pressure Effect ◽  
Binding Protein ◽  
Conformational Dynamics ◽  
Glucose Binding ◽  
The Stability ◽  
Glucose Binding Protein

scholarly journals Structural and Thermal Stability Characterization of Escherichia colid-Galactose/d-Glucose-Binding Protein

2008 ◽  
Vol 20 (1) ◽  
pp. 330-337 ◽  
Author(s):  
Sabato d'Auria ◽  
Fabrizio Alfieri ◽  
Maria Staiano ◽  
Fabrizio Pelella ◽  
Mose' Rossi ◽  
...  
Keyword(s):  
Thermal Stability ◽  
Binding Protein ◽  
Glucose Binding ◽  
Glucose Binding Protein

scholarly journals D-galactose/D-glucose-binding Protein from Escherichia coli as Probe for a Non-consuming Glucose Implantable Fluorescence Biosensor

Sensors ◽  
2007 ◽  
Vol 7 (10) ◽  
pp. 2484-2491 ◽  
Author(s):  
Viviana Scognamiglio ◽  
Vincenzo Aurilia ◽  
Nunzio Cennamo ◽  
Paola Ringhieri ◽  
Luisa Iozzino ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Binding Protein ◽  
Glucose Binding ◽  
Fluorescence Biosensor ◽  
Glucose Binding Protein

scholarly journals Structure and stability of D-galactose/D-glucose-binding protein. The role of D-glucose binding and Ca ion depletion

2010 ◽  
Vol 24 (3-4) ◽  
pp. 355-359 ◽  
Author(s):  
Olga V. Stepanenko ◽  
Olga I. Povarova ◽  
Olesya V. Stepanenko ◽  
Alexander V. Fonin ◽  
Irina M. Kuznetsova ◽  
...  
Keyword(s):  
Protein Unfolding ◽  
Binding Protein ◽  
Guanidine Hydrochloride ◽  
Protein Fluorescence ◽  
Structure And Stability ◽  
Glucose Binding ◽  
One Step ◽  
The Stability ◽  
Glucose Binding Protein

The effects of guanidine hydrochloride (GdnHCl) on the structure and stability of the D-galactose/D-glucose-binding protein fromEscherichia coli(GGBP) and its complex with D-glucose (GGBP/Glc) were investigated by intrinsic protein fluorescence and far-UV circular dichroism (CD). The role of calcium in the stability of the protein structure was also studied. It was shown that the processes of GGBP and GGBP/Glc unfolding induced by GdnHCl followed one-step reversible denaturation mechanism. The obtained data showed that the binding of glucose to GGBP resulted in an increase of the protein stability towards the actions of the GdnHCl which made protein unfolding more cooperative. The stabilities of GGBP alone, GGBP in the presence of glucose, GGBP-depleted calcium (GGBP-Ca), and GGBP/Glc-depleted calcium (GGBP/Glc-Ca) were characterized by difference of Gibbs free energies.

scholarly journals The role of the substrate on the mechanical and thermal stability of Pd thin films during hydrogen (de)sorption

2021 ◽  
Vol 46 (5) ◽  
pp. 4137-4153
Author(s):  
Neha Verma ◽  
Rob Delhez ◽  
Niek M. van der Pers ◽  
Frans D. Tichelaar ◽  
Amarante J. Böttger
Keyword(s):  
Thin Films ◽  
Thermal Stability ◽  
Thermal Stability Of
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