Crystal structure of the C67A mutant of isopentenyl diphosphate isomerase complexed with a mechanism-based irreversible inhibitor

2003 ◽  
Vol 54 (2) ◽  
pp. 216-221 ◽  
Author(s):  
J. Wouters ◽  
Y. Oudjama ◽  
V. Stalon ◽  
L. Droogmans ◽  
C.D. Poulter
Keyword(s):  
Crystal Structure ◽  
Isopentenyl Diphosphate ◽  
Irreversible Inhibitor ◽  
Isopentenyl Diphosphate Isomerase

scholarly journals Crystal Structure of Type 2 Isopentenyl Diphosphate Isomerase fromThermus thermophilusin Complex with Inorganic Pyrophosphate†

Biochemistry ◽  
2008 ◽  
Vol 47 (35) ◽  
pp. 9051-9053 ◽  
Author(s):  
Jérôme de Ruyck ◽  
Jenny Pouyez ◽  
Steven C. Rothman ◽  
Dale Poulter ◽  
Johan Wouters
Keyword(s):  
Crystal Structure ◽  
Isopentenyl Diphosphate ◽  
Inorganic Pyrophosphate ◽  
Isopentenyl Diphosphate Isomerase

scholarly journals Biosynthesis of pinene in purple non-sulfur photosynthetic bacteria

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Xiaomin Wu ◽  
Guang Ma ◽  
Chuanyang Liu ◽  
Xin-yuan Qiu ◽  
Lu Min ◽  
...  
Keyword(s):  
Fusion Protein ◽  
Heterologous Expression ◽  
Rhodobacter Sphaeroides ◽  
Photosynthetic Bacteria ◽  
Fine Chemicals ◽  
Isopentenyl Diphosphate ◽  
Isopentenyl Diphosphate Isomerase ◽  
Geranyl Diphosphate ◽  
Ribosome Binding ◽  
Geranyl Diphosphate Synthase

Abstract Background Pinene is a monoterpene, that is used in the manufacture of fragrances, insecticide, fine chemicals, and renewable fuels. Production of pinene by metabolic-engineered microorganisms is a sustainable method. Purple non-sulfur photosynthetic bacteria belong to photosynthetic chassis that are widely used to synthesize natural chemicals. To date, researches on the synthesis of pinene by purple non-sulfur photosynthetic bacteria has not been reported, leaving the potential of purple non-sulfur photosynthetic bacteria synthesizing pinene unexplored. Results Rhodobacter sphaeroides strain was applied as a model and engineered to express the fusion protein of heterologous geranyl diphosphate synthase (GPPS) and pinene synthase (PS), hence achieving pinene production. The reaction condition of pinene production was optimized and 97.51 μg/L of pinene was yielded. Then, genes of 1-deoxy-d-xylulose 5-phosphate synthase, 1-deoxy-d-xylulose 5-phosphate reductoisomerase and isopentenyl diphosphate isomerase were overexpressed, and the ribosome binding site of GPPS-PS mRNA was optimized, improving pinene titer to 539.84 μg/L. Conclusions In this paper, through heterologous expression of GPPS-PS, pinene was successfully produced in R. sphaeroides, and pinene production was greatly improved by optimizing the expression of key enzymes. This is the first report on pinene produce by purple non-sulfur photosynthetic bacteria, which expands the availability of photosynthetic chassis for pinene production.

scholarly journals Improved Squalene Production via Modulation of the Methylerythritol 4-Phosphate Pathway and Heterologous Expression of Genes from Streptomyces peucetius ATCC 27952 in Escherichia coli

2009 ◽  
Vol 75 (22) ◽  
pp. 7291-7293 ◽  
Author(s):  
Gopal Prasad Ghimire ◽  
Hei Chan Lee ◽  
Jae Kyung Sohng
Keyword(s):  
Escherichia Coli ◽  
Heterologous Expression ◽  
Farnesyl Diphosphate ◽  
Farnesyl Diphosphate Synthase ◽  
Isopentenyl Diphosphate ◽  
Isopentenyl Diphosphate Isomerase ◽  
Streptomyces Peucetius ◽  
E Coli ◽  
Expression Of Genes ◽  
Genes Encoding

ABSTRACT Putative hopanoid genes from Streptomyces peucetius were introduced into Escherichia coli to improve the production of squalene, an industrially important compound. High expression of hopA and hopB (encoding squalene/phytoene synthases) together with hopD (encoding farnesyl diphosphate synthase) yielded 4.1 mg/liter of squalene. This level was elevated to 11.8 mg/liter when there was also increased expression of dxs and idi, E. coli genes encoding 1-deoxy-d-xylulose 5-phosphate synthase and isopentenyl diphosphate isomerase.

Isopentenyl diphosphate isomerase, a cholesterol synthesizing enzyme, is localized in Lewy bodies

2015 ◽  
Vol 35 (5) ◽  
pp. 432-440 ◽  
Author(s):  
Keiko Nakamura ◽  
Fumiaki Mori ◽  
Kunikazu Tanji ◽  
Yasuo Miki ◽  
Masahito Yamada ◽  
...  
Keyword(s):  
Lewy Bodies ◽  
Isopentenyl Diphosphate ◽  
Isopentenyl Diphosphate Isomerase

Isopentenyl diphosphate isomerase and prenyltransferase activities in rubiaceous and apocynaceous cultures

1998 ◽  
Vol 48 (6) ◽  
pp. 961-969 ◽  
Author(s):  
Ana C Ramos-Valdivia ◽  
Robert van der Heijden ◽  
Robert Verpoorte
Keyword(s):  
Isopentenyl Diphosphate ◽  
Isopentenyl Diphosphate Isomerase

scholarly journals Characterization of an isopentenyl diphosphate isomerase involved in the juvenile hormone pathway in Aedes aegypti

2012 ◽  
Vol 42 (10) ◽  
pp. 751-757 ◽  
Author(s):  
Miguel E. Diaz ◽  
Jaime G. Mayoral ◽  
Horacio Priestap ◽  
Marcela Nouzova ◽  
Crisalejandra Rivera-Perez ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Juvenile Hormone ◽  
Isopentenyl Diphosphate ◽  
Isopentenyl Diphosphate Isomerase

Cloning and heterologous expression of isopentenyl diphosphate isomerase gene from Lycium chinense

2015 ◽  
Vol 25 (1) ◽  
pp. 40-48 ◽  
Author(s):  
Zhaodi Li ◽  
Jing Ji ◽  
Gang Wang ◽  
Tchouopou Lontchi Josine ◽  
Jiang Wu ◽  
...  
Keyword(s):  
Heterologous Expression ◽  
Isopentenyl Diphosphate ◽  
Lycium Chinense ◽  
Isopentenyl Diphosphate Isomerase
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