Relationship between protein structures and disulfide-bonding patterns

2003 ◽  
Vol 53 (1) ◽  
pp. 1-5 ◽  
Author(s):  
Chao-Chun Chuang ◽  
Chun-Yin Chen ◽  
Jinn-Moon Yang ◽  
Ping-Chiang Lyu ◽  
Jenn-Kang Hwang
Keyword(s):  
Protein Structures ◽  
Disulfide Bonding ◽  
Bonding Patterns

Mass Spectrometric Based Mapping of the Disulfide Bonding Patterns of Integrin α Chains†

Biochemistry ◽  
2003 ◽  
Vol 42 (44) ◽  
pp. 12950-12959 ◽  
Author(s):  
Oleg V. Krokhin ◽  
Keding Cheng ◽  
Sandra L. Sousa ◽  
Werner Ens ◽  
Kenneth G. Standing ◽  
...  
Keyword(s):  
Mass Spectrometric ◽  
Disulfide Bonding ◽  
Bonding Patterns

scholarly journals Disulfide bonding patterns and protein topologies

1993 ◽  
Vol 2 (1) ◽  
pp. 41-54 ◽  
Author(s):  
Craig J. Benham ◽  
M. Saleet Jafri
Keyword(s):  
Disulfide Bonding ◽  
Bonding Patterns

scholarly journals The effect of meat processing methods on changes in disulfide bonding and alteration of protein structures: impact on protein digestion products

RSC Advances ◽  
2018 ◽  
Vol 8 (31) ◽  
pp. 17595-17605 ◽  
Author(s):  
Jing He ◽  
Guanghong Zhou ◽  
Yun Bai ◽  
Chao Wang ◽  
Shuran Zhu ◽  
...  
Keyword(s):  
Protein Structure ◽  
Disulfide Bonds ◽  
Protein Structures ◽  
Protein Digestion ◽  
Meat Processing ◽  
Processing Methods ◽  
Disulfide Bonding ◽  
Structure Changes ◽  
Different Levels

Processing caused different levels of formation of disulfide bonds, and associated it with protein structure changes and digested products.

Kinetic and Structural Properties of Disulfide Engineered Phospholipase A2:  Insight into the Role of Disulfide Bonding Patterns†,‡

Biochemistry ◽  
2005 ◽  
Vol 44 (9) ◽  
pp. 3369-3379 ◽  
Author(s):  
Bao-Zhu Yu ◽  
Ying H. Pan ◽  
Marcel J. W. Janssen ◽  
Brian J. Bahnson ◽  
Mahendra K. Jain
Keyword(s):  
Phospholipase A2 ◽  
Structural Properties ◽  
Disulfide Bonding ◽  
Insight Into ◽  
Bonding Patterns

Heat-Induced Redistribution of Disulfide Bonds in Milk Proteins. 2. Disulfide Bonding Patterns between Bovine β-Lactoglobulin and κ-Casein

2004 ◽  
Vol 52 (25) ◽  
pp. 7669-7680 ◽  
Author(s):  
Edwin K. Lowe ◽  
Skelte G. Anema ◽  
Annie Bienvenue ◽  
Michael J. Boland ◽  
Lawrence K. Creamer ◽  
...  
Keyword(s):  
Disulfide Bonds ◽  
Milk Proteins ◽  
Disulfide Bonding ◽  
Β Lactoglobulin ◽  
Bonding Patterns

Insights into the biochemical and functional characterization of sortase E transpeptidase of Corynebacterium glutamicum

2019 ◽  
Vol 476 (24) ◽  
pp. 3835-3847 ◽  
Author(s):  
Aliyath Susmitha ◽  
Kesavan Madhavan Nampoothiri ◽  
Harsha Bajaj
Keyword(s):  
Protein Engineering ◽  
Cell Attachment ◽  
Functional Characterization ◽  
Protein Structures ◽  
Structural Similarity ◽  
Surface Proteins ◽  
Sortase A ◽  
Peptide Cleavage ◽  
New Findings

Most Gram-positive bacteria contain a membrane-bound transpeptidase known as sortase which covalently incorporates the surface proteins on to the cell wall. The sortase-displayed protein structures are involved in cell attachment, nutrient uptake and aerial hyphae formation. Among the six classes of sortase (A–F), sortase A of S. aureus is the well-characterized housekeeping enzyme considered as an ideal drug target and a valuable biochemical reagent for protein engineering. Similar to SrtA, class E sortase in GC rich bacteria plays a housekeeping role which is not studied extensively. However, C. glutamicum ATCC 13032, an industrially important organism known for amino acid production, carries a single putative sortase (NCgl2838) gene but neither in vitro peptide cleavage activity nor biochemical characterizations have been investigated. Here, we identified that the gene is having a sortase activity and analyzed its structural similarity with Cd-SrtF. The purified enzyme showed a greater affinity toward LAXTG substrate with a calculated KM of 12 ± 1 µM, one of the highest affinities reported for this class of enzyme. Moreover, site-directed mutation studies were carried to ascertain the structure functional relationship of Cg-SrtE and all these are new findings which will enable us to perceive exciting protein engineering applications with this class of enzyme from a non-pathogenic microbe.

Sign in / Sign up

Export Citation Format

Share Document