scholarly journals Inhibition of prostatic epithelial cell proliferation by a factor secreted specifically by prostatic stromal cells

The Prostate ◽  
1995 ◽  
Vol 26 (3) ◽  
pp. 123-132 ◽  
Author(s):  
Anko Kooistra ◽  
Josée J. König ◽  
Diederick M. Keizer ◽  
Johan C. Romijn ◽  
Fritz H. Schröder
Keyword(s):  
Cell Proliferation ◽  
Epithelial Cell ◽  
Stromal Cells ◽  
Epithelial Cell Proliferation ◽  
Prostatic Epithelial Cell

Androgen-independent Effects of Serenoa repens Extract (Prostasan®) on Prostatic Epithelial Cell Proliferation and Inflammation

2011 ◽  
Vol 26 (2) ◽  
pp. 259-264 ◽  
Author(s):  
Diego Iglesias-Gato ◽  
Tober Carsten ◽  
Mattias Vesterlund ◽  
Ake Pousette ◽  
Roland Schoop ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Epithelial Cell ◽  
Epithelial Cell Proliferation ◽  
Serenoa Repens ◽  
Independent Effects ◽  
Prostatic Epithelial Cell ◽  
Androgen Independent

scholarly journals Juxtacrine Activity of Estrogen Receptor α in Uterine Stromal Cells is Necessary for Estrogen-Induced Epithelial Cell Proliferation

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Wipawee Winuthayanon ◽  
Sydney L. Lierz ◽  
Karena C. Delarosa ◽  
Skylar R. Sampels ◽  
Lauren J. Donoghue ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Estrogen Receptor ◽  
Epithelial Cell ◽  
Stromal Cells ◽  
Estrogen Receptor Α ◽  
Epithelial Cell Proliferation

Vasoactive intestinal peptide (VIP) stimulates rat prostatic epithelial cell proliferation

The Prostate ◽  
2001 ◽  
Vol 47 (4) ◽  
pp. 285-292 ◽  
Author(s):  
Maria G. Juarranz ◽  
Guillermo Bodega ◽  
Juan C. Prieto ◽  
Luis G. Guijarro
Keyword(s):  
Cell Proliferation ◽  
Epithelial Cell ◽  
Vasoactive Intestinal Peptide ◽  
Epithelial Cell Proliferation ◽  
Prostatic Epithelial Cell

Establishment of a Primary Culture Model of Mouse Uterine and Vaginal Stroma for Studying In Vitro Estrogen Effects

2006 ◽  
Vol 231 (3) ◽  
pp. 303-310 ◽  
Author(s):  
Keiko Inada ◽  
Shinji Hayashi ◽  
Taisen Iguchi ◽  
Tomomi Sato
Keyword(s):  
Cell Proliferation ◽  
Epithelial Cell ◽  
Primary Culture ◽  
Stromal Cells ◽  
Renal Capsule ◽  
Epithelial Cell Proliferation ◽  
Culture Model ◽  
Brdu Labeling

Effects of 17β-estradiol (E2) on uterine and vaginal epithelial cell proliferation could be mediated by stromal cell-derived paracrine factors. To study the epithelial-stromal interactions in mice, an in vitro model of uterine and vaginal stromal cells of immature mice is essential. Therefore, we established a primary culture model of stromal cells both from uterus and vagina and examined the effect of E2 on proliferation of cultured stromal cells. We found that E2 stimulated proliferation of stromal cells from both organs in vitro, showing an increase in the number of cells and the percentage of 5-bromo-2'-deoxyurldine (BrdU)-labeled cells. Interestingly, vaginal stromal cells responded to lower E2 than uterine stromal cells in proliferation (10−12 M vs. 10−8 M) and BrdU labeling (10−14 -10−10 M vs. 10−10 - 10−6 M). To examine the effect of E2 in vivo, cells were grafted into the subrenal capsule of the host mice and grown for 2 weeks. The BrdU labeling in cultured stromal cells was increased by E2 in vivo. To examine the effect of cultured stromal cells on epithelial cell proliferation, uterine and vaginal epithelium of adult mice were separated, recombined with the cultured stromal cells, and grafted under the renal capsule of hosts for 3 weeks. Epithelial cells recombined with cultured stromal cells showed simple columnar morphology in uterine grafts and stratified and keratinized morphology in vaginal grafts under the influence of the hormonal environment of the hosts. The BrdU labeling in epithelial cells was increased by E2, suggesting that cultured stromal cells can stimulate epithelial cell proliferation. In conclusion, we established a primary culture model of uterine and vaginal stromal cells, which can be mitogenically stimulated by E2 in vitro and in vivo after being grafted under the renal capsule. This culture system will be useful for investigating the underlying molecular mechanisms of uterine and vaginal epithelial-stromal interactions.

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