scholarly journals Species- and organ-specificity of secretory proteins derived from human prostate and seminal vesicles

The Prostate ◽  
1990 ◽  
Vol 17 (1) ◽  
pp. 31-40 ◽  
Author(s):  
G. Aumüller ◽  
J. Seitz ◽  
H. Lilja ◽  
P.-A. Abrahamsson ◽  
H. Der Von Kammer ◽  
...  
Keyword(s):  
Seminal Vesicles ◽  
Human Prostate ◽  
Organ Specificity ◽  
Secretory Proteins

Induction of functional cytodifferentiation in the epithelium of tissue recombinants. I. Homotypic seminal vesicle recombinants

Development ◽  
1989 ◽  
Vol 106 (2) ◽  
pp. 219-234 ◽  
Author(s):  
S.J. Higgins ◽  
P. Young ◽  
J.R. Brody ◽  
G.R. Cunha
Keyword(s):  
Seminal Vesicle ◽  
Time Course ◽  
Full Range ◽  
Seminal Vesicles ◽  
Neonatal Rat ◽  
Secretory Proteins ◽  
Functional Variation ◽  
Adult Rats ◽  
Normal Functional

Functional cytodifferentiation of seminal vesicle epithelium was investigated in tissue recombinants. Neonatal rat and mouse seminal vesicles were separated into epithelium and mesenchyme using trypsin. Epithelium and mesenchyme were then recombined in vitro to form interspecific rat/mouse homotypic recombinants. Growth as renal grafts in adult male athymic mice resulted in seminal vesicle morphogenesis in 70% of the recombinants (the remaining 30% failed to grow). Functional cytodifferentiation was judged by the expression of the major androgen-dependent secretory proteins characteristic of the seminal vesicles of adult rats and mice. Antibodies specific for each of these proteins were used to screen tissue sections by immunocytochemistry and to probe protein extracts by immunoblotting techniques. The heterospecific recombinants synthesized the full range of seminal vesicle secretory proteins that typifies the species providing the epithelium of the recombinant, not the mesenchyme. There was little functional variation between individual recombinants. The time course of development corresponded to that of intact neonatal seminal vesicles grown under the same conditions. Morphogenesis and functional cytodifferentiation were not evident after one week, but were well advanced after two weeks. Seminal vesicle recombinants grown for three weeks were indistinguishable morphologically and functionally from normal adult seminal vesicles. In addition, the ability of adult seminal vesicle epithelium to be induced to proliferate was examined. In association with neonatal seminal vesicle mesenchyme, the epithelium of the adult seminal vesicle proliferated and retained its normal functional activity. Thus, seminal vesicle functional cytodifferentiation can be faithfully reproduced in homotypic tissue recombinants. The methods used in this study will be used to investigate seminal vesicle development in instructive inductions of heterotypic epithelia.

Induction of functional cytodifferentiation in the epithelium of tissue recombinants. II. Instructive induction of Wolffian duct epithelia by neonatal seminal vesicle mesenchyme

Development ◽  
1989 ◽  
Vol 106 (2) ◽  
pp. 235-250 ◽  
Author(s):  
S.J. Higgins ◽  
P. Young ◽  
G.R. Cunha
Keyword(s):  
Seminal Vesicle ◽  
Normal Development ◽  
Seminal Vesicles ◽  
Wolffian Duct ◽  
Secretory Proteins ◽  
Ductus Deferens ◽  
Tissue Sections ◽  
Duct Epithelium ◽  
Mouse Tissues ◽  
Renal Grafts

When grown as renal grafts in adult male hosts, the upper (cranial), middle and lower (caudal) portions of fetal mouse and rat Wolffian ducts developed into epididymis, epididymis plus ductus deferens, and seminal vesicle, respectively. In heterotypic tissue recombinants, the epithelia from upper and middle Wolffian ducts were instructively induced to undergo seminal vesicle morphogenesis by neonatal seminal vesicle mesenchyme. Functional cytodifferentiation was examined in these recombinants using antibodies against major androgen-dependent, seminal vesicle-specific secretory proteins. The instructively induced Wolffian duct epithelia synthesized normal amounts of all of the secretory proteins characteristic of mature seminal vesicles, as judged by immunocytochemistry on tissue sections and gel electrophoresis plus immunoblotting of secretions extracted from the recombinants. In heterospecific recombinants composed of rat and mouse tissues, the seminal vesicle proteins induced were specific for the species that had provided the epithelium. This showed that the seminal vesicle epithelium in the recombinants was derived from instructively induced Wolffian duct epithelium and not from epithelial contamination of the mesenchymal inductor. Upper Wolffian duct epithelium, instructively induced to undergo seminal vesicle morphogenesis, did not express epididymis-specific secretory proteins, showing that its normal development had been simultaneously repressed.

scholarly journals Isolation and characterisation of genes for androgen-responsive secretory proteins of rat seminal vesicles

1983 ◽  
Vol 11 (4) ◽  
pp. 917-930 ◽  
Author(s):  
Charles McDonald ◽  
Lindsay Williams ◽  
Pamela McTurk ◽  
Frances Fuller ◽  
Elizabeth Mclntosh ◽  
...  
Keyword(s):  
Seminal Vesicles ◽  
Secretory Proteins

Metabolism of Testosterone in the Human Prostate and Seminal Vesicles

1977 ◽  
Vol 11 (1) ◽  
pp. 1-6 ◽  
Author(s):  
O. Djøseland ◽  
K. J. Tveter ◽  
A. Attramadal ◽  
V. Hansson ◽  
H. N. Haugen ◽  
...  
Keyword(s):  
Seminal Vesicles ◽  
Human Prostate

Neonatal seminal vesicle mesenchyme induces a new morphological and functional phenotype in the epithelia of adult ureter and ductus deferens

Development ◽  
1991 ◽  
Vol 111 (1) ◽  
pp. 145-158 ◽  
Author(s):  
G.R. Cunha ◽  
P. Young ◽  
S.J. Higgins ◽  
P.S. Cooke
Keyword(s):  
Epithelial Cells ◽  
Seminal Vesicle ◽  
Developmental Plasticity ◽  
Androgen Receptors ◽  
Seminal Vesicles ◽  
Neonatal Mouse ◽  
Secretory Proteins ◽  
Complete Spectrum ◽  
Ductus Deferens ◽  
Functional Phenotype

Mesenchyme from neonatal mouse and rat seminal vesicles (SVM) was grown in association with postnatal (adult) epithelial cells from the ureter (URE) and ductus deferens (DDE) in chimeric tissue recombinants composed of mouse mesenchyme and rat epithelium or vice versa. Functional cytodifferentiation was examined in these SVM + URE and SVM + DDE tissue recombinants with antibodies against major androgen-dependent seminal-vesicle-specific secretory proteins. Adult DDE and URE were induced to express seminal cytodifferentiation and produced the complete spectrum of major seminal vesicle secretory (SVS) proteins. The SVS proteins produced were specific for the species that provided the epithelium. In the case of SVM + URE recombinants, the URE, which normally lacks androgen receptors (AR), expressed AR. These results demonstrate that adult epithelial cells retain a developmental plasticity equivalent to their undifferentiated fetal counterparts and are capable of being reprogrammed to express a completely new morphological, biochemical and functional phenotype.

IN VITRO UPTAKE OF TRITIATED TESTOSTERONE AND OESTRADIOL BY THE LATERAL LOBE OF THE HYPERTROPHIED HUMAN PROSTATE AND THE ACCESSORY SEX GLANDS OF THE RAT – A PILOT STUDY

1969 ◽  
Vol 61 (1) ◽  
pp. 25-32 ◽  
Author(s):  
Carl-Evert Jonsson
Keyword(s):  
Cyproterone Acetate ◽  
Lateral Lobe ◽  
Blocking Effect ◽  
Seminal Vesicles ◽  
Human Prostate ◽  
Ventral Prostate ◽  
Target Tissue ◽  
Immature Rat ◽  
Accessory Sex Glands

ABSTRACT Slices of the lateral lobe of hypertrophied human prostate were incubated with tritium labelled testosterone or oestradiol. After adding non-labelled testosterone in excess there was a marked reduction in uptake of tritiated testosterone, but neither non-radioactive oestradiol nor the antiandrogenically active Cyproterone acetate® had any effect on uptake of radioactivity. After pre-incubation with testosterone, oestradiol or Cyproterone acetate®, followed by washing and reincubation with tritiated testosterone, no blocking effect could be demonstrated. Addition of non-radioactive oestradiol in excess did not affect the uptake of tritiated oestradiol. In the seminal vesicles and ventral prostate of the immature rat there was an accumulation of tritiated testosterone as compared to the diaphragm. Non-radioactive testosterone in excess reduced this uptake in the target tissue.

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