scholarly journals Altered prostate epithelial development and IGF-1 signal in mice lacking the androgen receptor in stromal smooth muscle cells

The Prostate ◽  
2010 ◽  
Vol 71 (5) ◽  
pp. 517-524 ◽  
Author(s):  
Shengqiang Yu ◽  
Caixia Zhang ◽  
Chiu-Chun Lin ◽  
Yuanjie Niu ◽  
Kuo-Pao Lai ◽  
...  
1993 ◽  
Vol 90 (2) ◽  
pp. 219-229 ◽  
Author(s):  
Néstor F. González-Cadavid ◽  
Dolores Vernet ◽  
Angela Fuentes Navarro ◽  
JoséAntonio Rodŕiguez ◽  
Ronald S. Swerdloff ◽  
...  

2017 ◽  
Vol 1 (suppl_1) ◽  
pp. 777-777
Author(s):  
M. Nanao-Hamai ◽  
B. Son ◽  
T. Hashizume ◽  
S. Ogawa ◽  
M. Akishita

Endocrinology ◽  
2017 ◽  
Vol 159 (2) ◽  
pp. 945-956 ◽  
Author(s):  
Nahuel Peinetti ◽  
María Victoria Scalerandi ◽  
Mariana Micaela Cuello Rubio ◽  
Carolina Leimgruber ◽  
Juan Pablo Nicola ◽  
...  

Author(s):  
J.M. Minda ◽  
E. Dessy ◽  
G. G. Pietra

Pulmonary lymphangiomyomatosis (PLAM) is a rare disease occurring exclusively in women of reproductive age. It involves the lungs, lymph nodes and lymphatic ducts. In the lungs, it is characterized by the proliferation of smooth muscle cells around lymphatics in the bronchovascular bundles, lobular septa and pleura The nature of smooth muscle proliferation in PLAM is still unclear. Recently, reactivity of the smooth muscle cells for HMB-45, a melanoma-related antigen has been reported by immunohistochemistry. The purpose of this study was the ultrastructural localization of HMB-45 immunoreactivity in these cells using gold-labeled antibodies.Lung tissue from three cases of PLAM, referred to our Institution for lung transplantation, was embedded in either Poly/Bed 812 post-fixed in 1% osmium tetroxide, or in LR White, without osmication. For the immunogold technique, thin sections were placed on Nickel grids and incubated with affinity purified, monoclonal anti-melanoma antibody HMB-45 (1:1) (Enzo Diag. Co) overnight at 4°C. After extensive washing with PBS, grids were treated with Goat-anti-mouse-IgG-Gold (5nm) (1:10) (Amersham Life Sci) for 1 hour, at room temperature.


Sign in / Sign up

Export Citation Format

Share Document