scholarly journals Tissue culture media supplemented with 10% fetal calf serum contains a castrate level of testosterone

The Prostate ◽  
2009 ◽  
Vol 69 (16) ◽  
pp. 1724-1729 ◽  
Author(s):  
J.P. Michiel Sedelaar ◽  
John T. Isaacs
Keyword(s):  
Tissue Culture ◽  
Fetal Calf Serum ◽  
Culture Media ◽  
Calf Serum ◽  
Tissue Culture Media

scholarly journals The Modification of Factor VIII Procoagulant Activity in Tissue Culture Media Supplemented with Fetal Calf Serum

1977 ◽  
Author(s):  
Nancy W. Stead ◽  
P. A. McKee
Keyword(s):  
Endothelial Cells ◽  
Factor Viii ◽  
Fetal Calf Serum ◽  
Culture Media ◽  
Umbilical Vein ◽  
Calf Serum ◽  
Procoagulant Activity ◽  
Growth Media ◽  
Tissue Culture Media ◽  
Plasma Factor

Cultured human umbilical vein endothelial cells produce a protein that reacts with rabbit antibody to purified plasma factor VIII (FVIII)/von Willebrand factor (vWF) and has vWF activity, but not FVIII procoagulant activity (PCA) (Jaffe et al. JCI 52: 2757, 1973) . In our laboratory endothelial cell cultures produce vWF activity that increases to 3 µg/culture by 72 hrs; partially purified protein from culture media forms an immunoprecipitin line of identity with human, but not bovine, plasma FVIII/vWF. Of the 3 functions of plasma FVIII/vWF protein, PCA, but not vWF activity or antigenicity, is modified by trace proteases. An assay that measures hydrolysis of p-nitroaniline from the tripeptide Bz-phe-val-arg-p-nitroanilide detects trace proteases in growth media; 0.001 NIH u thrombin releases measurable quantities of p-nitroaniline. Culture media without fetal calf serum (FCS) did not hydrolyze the tripeptide. Media 20% in: FCS, FCS heated to 56° for 30 min, FCS made 1.7×10-3M diisopropylfluorphosphate (DFP), FCS and 4.4x10-8 moles soybean trypsin inhibitor, or FCS and 4.4xl0-8 moles Trasylol releases 3.5x10-6, 3.6x10-7, 3.6xl0-7, 3.5xl0-6 and 2.8xl0-6 mmole of p-nitroaniline per ml of media per min. PCA of FVIII/vWF molecule is measured by one stage (PCA1) and two stage (PCA2) assays. Although >85% PCA1 or PCA2 of purified plasma FVIIl/vWF added to media without FCS remains at 6 hours, varying amounts of PCA1 and only 10% PCA2 of the FVIII/vWF added to media 20% in FCS remained in 6 hrs. Hence endothelial cells make a protein with vWF activity and FVIII/vWF antigenicity; but were the protein to have PCA at the time of release, trace proteases in the growth media could quickly destroy it.

Fractional Factorial Design for Optimization of the SELDI Protocol for Human Adipose Tissue Culture Media

2007 ◽  
Vol 23 (1) ◽  
pp. 217-224 ◽  
Author(s):  
E. Szalowska ◽  
S.A.F.T. vanHijum ◽  
H. Roelofsen ◽  
A. Hoek ◽  
R.J. Vonk ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Adipose Tissue ◽  
Factorial Design ◽  
Fractional Factorial Design ◽  
Culture Media ◽  
Human Adipose Tissue ◽  
Fractional Factorial ◽  
Tissue Culture Media

scholarly journals Reversible oxidant-induced increases in albumin transfer across cultured endothelium: alterations in cell shape and calcium homeostasis

Blood ◽  
1985 ◽  
Vol 65 (3) ◽  
pp. 605-614
Author(s):  
DM Shasby ◽  
SE Lind ◽  
SS Shasby ◽  
JC Goldsmith ◽  
GW Hunninghake
Keyword(s):  
Tissue Culture ◽  
Xanthine Oxidase ◽  
Calcium Homeostasis ◽  
Actin Filaments ◽  
Cell Shape ◽  
Culture Media ◽  
Extracellular Calcium ◽  
Lanthanum Chloride ◽  
Tissue Culture Media ◽  
Induced Changes

To determine whether reactive oxygen molecules could directly and reversibly increase the transfer of albumin across an endothelial barrier, we measured albumin transfer across monolayers of endothelium cultured on micropore filters before and after exposure to xanthine and xanthine oxidase. Xanthine and xanthine oxidase increased endothelial albumin transfer in a dose-dependent fashion. Parallel phase contrast and fluorescence microscopy demonstrated retraction of adjacent cells from one another and disruption of the actin filaments. The oxidant- induced increases in albumin transfer and changes in cell shape were reversed by removing xanthine oxidase and then incubating the monolayers for 3 1/2 hours in tissue culture media enriched with fetal bovine serum. However, incubation in tissue culture media without serum resulted in progressive injury and cell death. Hence, the brief exposure to oxidants initiated a progressive injury process that was reversed by incubation in serum. Because intracellular and extracellular calcium are important determinants of cell shape, and because some oxidized membrane lipids act as calcium ionophores, we asked whether oxidants altered endothelial calcium homeostasis. Xanthine-xanthine oxidase increased release of 45Ca++ from preloaded cells. The calcium antagonist lanthanum chloride prevented xanthine- xanthine oxidase increases in endothelial albumin transfer and prevented the changes in cell shape; chelation of extracellular calcium inhibited lysis of endothelium by xanthine-xanthine oxidase; and the calcium ionophore A23187 increased endothelial albumin transfer and mimicked the oxidant-induced changes in cell shape. Lanthanum chloride inhibited these effects of A23187. These data suggest that oxygen radicals can reversibly increase endothelial permeability to macromolecules, that this is associated with reversible changes in endothelial cell shape and actin filaments, and that the changes in cell shape are related to oxidant-induced changes in endothelial calcium homeostasis.

Retinoic acid increases the sensitivity of the rat embryo fibroblast transformation assay

1988 ◽  
Vol 8 (4) ◽  
pp. 1845-1848
Author(s):  
T D Halazonetis ◽  
C Daugherty ◽  
P Leder
Keyword(s):  
Tissue Culture ◽  
Retinoic Acid ◽  
Culture Media ◽  
Rat Embryo ◽  
Transformation Assay ◽  
Tissue Culture Media ◽  
Focus Assay ◽  
Fibroblast Transformation

The rat embryo fibroblast focus assay is used to evaluate the transforming potential of several oncogenes. The sensitivity of this assay increased fivefold when retinoic acid was added to tissue culture media. Retinoic acid probably acts by selectively inhibiting the proliferation of nontransformed cells.

scholarly journals Usefulness of serology for the evaluation of Trypanosoma cruzi transmission in endemic areas of Chagas' disease

1989 ◽  
Vol 22 (3) ◽  
pp. 119-124 ◽  
Author(s):  
Roberto Chuit ◽  
Elisabet Subias ◽  
Analia C. Pérez ◽  
Irene Paulone ◽  
Cristina Wisnivesky-Colli ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Trypanosoma Cruzi ◽  
Chagas Disease ◽  
Rural Areas ◽  
Culture Media ◽  
Previous Treatment ◽  
Insecticide Treatment ◽  
Tissue Culture Media ◽  
Indirect Hemagglutination ◽  
History Of

Thirteen communities from 7 Argentinian provinces were selected for the evaluation of serology as an indicator of transmission of Chagas disease. Of the communities appraised, 6 did not have a history of previous treatment with insecticides and 7 had received sporadic or continuous insecticide treatment. The inhabitants of 20% of the houses of each locality were studied by serology. The samples were obtained byfinger pricking and 50 fil of blood were mixed with 150μl of 50% glycerine solution in tissue culture media to be assayed by Indirect Hemagglutination and Indirect Immunofluorescence tests. In untreated areas, the prevalence of infection in infants 0-4 years old was 17.5%, reaching to over 22% for the 5-9 year old group, and to 33.3% in 10-14 year old individuals. The prevalence in treated and surveyed areas was 2.6% in 0-4 year old children, 5.4% in 5-9 year old and 6,2% in 10-14 year old youngsters. The differences between both areas were statistically significant (p < 0.005). This study favors serology as a valid indicator for the evaluation of transmission of Chagas disease in rural areas.

scholarly journals The Use of Bean Sprout Extract as Supplement for the Growth of Plaintain Unti Sayang (Musa paradisiaca L.) by Tissue Culture

2014 ◽  
Vol 2 (1) ◽  
pp. 99
Author(s):  
Nirwana Jufri ◽  
Dr. Abdullah ◽  
Devi Susanti
Keyword(s):  
Tissue Culture ◽  
Root Length ◽  
Culture Media ◽  
Leaf Number ◽  
Wide Field ◽  
Root Number ◽  
Bean Sprout ◽  
Musa Paradisiaca ◽  
Tissue Culture Media ◽  
Growth Quality

One of the problems in plaintain development is on the plant breeding that has been conducted convensionally by using seedlings, so that it requires time and wide field, different treatment, and potentially brings pests and deseases. The bean sprout extract is potentially useful in improving the explant growth quality of plaintain by tissue culture. This study aims to know the influence of bean sprout extract concentration as supplement in tissue culture media upon the explant growth of plaintain Unti sayang (Musa paradisiaca L). There are 4 dosages of bean sprout extract as control. The data was analyzed by using ANOVA and was continued by using BNJ test. The result of the experiment shows that the bean sprout extract influences significatly on the plantlets height, leaf number, root length, and root number. The dosage of 100 g/l and 200 g/l bean sprout extracts provide the best result on the experimenting component of plantlet height, leaf number, root length, and root number. Meanwhile, the experimenting component of leaf number and the wet weight of the plantlets provide the best result on MS without any addition of bean sprout extract.

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