The expression of xenobiotic-metabolizing enzymes in human prostate and in prostate epithelial cells (PECs) derived from primary cultures

The Prostate ◽  
2006 ◽  
Vol 66 (8) ◽  
pp. 876-885 ◽  
Author(s):  
S.Z. Al-Buheissi ◽  
K.J. Cole ◽  
A. Hewer ◽  
V. Kumar ◽  
R.L. Bryan ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Primary Cultures ◽  
Human Prostate ◽  
Metabolizing Enzymes ◽  
Xenobiotic Metabolizing Enzymes ◽  
Prostate Epithelial Cells

Testosterone metabolism in primary cultures of human prostate epithelial cells and fibroblasts

1995 ◽  
Vol 55 (3-4) ◽  
pp. 375-383 ◽  
Author(s):  
Sylvie De´los ◽  
Jean-Louis Carsol ◽  
Evelyne Ghazarossian ◽  
Jean-Pierre Raynaud ◽  
Pierre-Marie Martin
Keyword(s):  
Epithelial Cells ◽  
Primary Cultures ◽  
Human Prostate ◽  
Testosterone Metabolism ◽  
Prostate Epithelial Cells

scholarly journals Increased expression of type 2 3α-hydroxysteroid dehydrogenase/type 5 17β-hydroxysteroid dehydrogenase (AKR1C3) and its relationship with androgen receptor in prostate carcinoma

2006 ◽  
Vol 13 (1) ◽  
pp. 169-180 ◽  
Author(s):  
K-M Fung ◽  
E N S Samara ◽  
C Wong ◽  
A Metwalli ◽  
R Krlin ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Androgen Receptor ◽  
Epithelial Cells ◽  
Prostate Carcinoma ◽  
Primary Cultures ◽  
Hydroxysteroid Dehydrogenase ◽  
Human Prostate ◽  
Normal Prostate ◽  
Prostate Epithelial Cells

Type 2 3α-hydroxysteroid dehydrogenase (3α-HSD) is a multi-functional enzyme that possesses 3α-, 17β- and 20α-HSD, as well as prostaglandin (PG) F synthase activities and catalyzes androgen, estrogen, progestin and PG metabolism. Type 2 3α-HSD was cloned from human prostate, is a member of the aldo-keto reductase (AKR) superfamily and was named AKR1C3. In androgen target tissues such as the prostate, AKR1C3 catalyzes the conversion of Δ4-androstene-3,17-dione to testosterone, 5α-dihydrotestosterone to 5α-androstane-3α,17β-diol (3α-diol), and 3α-diol to androsterone. Thus AKR1C3 may regulate the balance of androgens and hence trans-activation of the androgen receptor in these tissues. Tissue distribution studies indicate that AKR1C3 transcripts are highly expressed in human prostate. To measure AKR1C3 protein expression and its distribution in the prostate, we raised a monoclonal antibody specifically recognizing AKR1C3. This antibody allowed us to distinguish AKR1C3 from other AKR1C family members in human tissues. Immunoblot analysis showed that this monoclonal antibody binds to one species of protein in primary cultures of prostate epithelial cells and in LNCaP prostate cancer cells. Immunohistochemistry with this antibody on human prostate detected strong nuclear immunoreactivity in normal stromal and smooth muscle cells, perineurial cells, urothelial (transitional) cells, and endothelial cells. Normal prostate epithelial cells were only faintly immunoreactive or negative. Positive immunoreactivity was demonstrated in primary prostatic adenocarcinoma in 9 of 11 cases. Variable increases in immunoreactivity for AKR1C3 was also demonstrated in non-neoplastic changes in the prostate including chronic inflammation, atrophy and urothelial (transitional) cell metaplasia. We conclude that elevated expression of AKR1C3 is highly associated with prostate carcinoma. Although the biological significance of elevated AKR1C3 in prostatic carcinoma is uncertain, AKR1C3 may be responsible for the trophic effects of androgens and/or PGs on prostatic epithelial cells.

MP36-16 THE EXPRESSION OF BETA-DEFENSIN 131 PROMOTES INNATE IMMUNITY IN HUMAN PROSTATE EPITHELIAL CELLS

2016 ◽  
Vol 195 (4S) ◽  
Author(s):  
Jung Hoon Kim ◽  
Kyeoung-Hwa Kim ◽  
Hae Jong Kim ◽  
Jaekhyouk Lee ◽  
Soon Chul Myung ◽  
...  
Keyword(s):  
Innate Immunity ◽  
Epithelial Cells ◽  
Human Prostate ◽  
Prostate Epithelial Cells

E-cadherin expression and PSA secretion in human prostate epithelial cells

2001 ◽  
Vol 29 (4) ◽  
pp. 287-292 ◽  
Author(s):  
Diane Krill ◽  
Angela Thomas ◽  
Su-Ping Wu ◽  
Rajiv Dhir ◽  
Michael J. Becich
Keyword(s):  
Epithelial Cells ◽  
Human Prostate ◽  
Prostate Epithelial Cells ◽  
E Cadherin
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