scholarly journals Unfolding and refolding of dimeric creatine kinase equilibrium and kinetic studies

1998 ◽  
Vol 7 (12) ◽  
pp. 2631-2641 ◽  
Author(s):  
Ying-Xin Fan ◽  
Jun-Mei Zhou ◽  
Chen-Lu Tsou ◽  
Hiroshi Kihara
Keyword(s):  
Creatine Kinase ◽  
Kinetic Studies ◽  
Creatine Kinase Equilibrium

Fish muscle energy metabolism measured during hypoxia and recovery: an in vivo 31P-NMR study

1995 ◽  
Vol 268 (5) ◽  
pp. R1178-R1187 ◽  
Author(s):  
V. van Ginneken ◽  
G. van den Thillart ◽  
A. Addink ◽  
C. Erkelens
Keyword(s):  
Creatine Kinase ◽  
Fish Muscle ◽  
High Energy ◽  
Anaerobic Glycolysis ◽  
O2 Consumption ◽  
Nmr Study ◽  
Creatine Kinase Equilibrium ◽  
Creatine Kinase Reaction ◽  
Muscle Energy Metabolism

Three fish species were exposed to graded hypoxia levels and allowed to recover. Levels of high-energy phosphate compounds in epaxial white muscle were monitored by in vivo 31P nuclear magnetic resonance (NMR) spectroscopy. Furthermore, O2 consumption of the animals was measured. With increasing hypoxia load, metabolic parameters started to change in the following order: phosphocreatine (PCr)-to-Pi ratio (decrease), O2 consumption (decrease), [PCr] (decrease), intracellular pH (pHi; decrease), Pi (increase), free ADP concentration ([ADP]free; increase), [ATP] (decrease). PCr levels fell with the PO2. After each increment, the [PCr] reached a stable plateau value while, in some cases, a recovery was observed. This recovery could be explained because the balance between anaerobic and aerobic metabolism is continuously fluctuating during hypoxia as a consequence of changes in the activity of the fish. Consequently the [ADP]free are fluctuating, resulting in an activation of the creatine kinase reaction and the anaerobic glycolysis. In all three species, anaerobic glycolysis was activated, but in contrast to anoxia exposure, metabolic suppression was absent. The changes of [ADP]free and [H+] (which influences the position of the creatine kinase equilibrium) are species dependent. Species differences observed in the other parameters were small. It is concluded that the pattern of the activation of anaerobic metabolism under deep hypoxia is different from that under anoxia.

Kinetic studies on the inhibition of creatine kinase activity by branched‐chain α‐amino acids in the brain cortex of rats

2003 ◽  
Vol 21 (3) ◽  
pp. 145-151 ◽  
Author(s):  
Carmen Pilla ◽  
Rui Felipe Oliveira Cardozo ◽  
Paula Karine Barcelos Dornelles ◽  
Carlos Severo Dutra‐Filho ◽  
Angela Terezinha Souza Wyse ◽  
...  
Keyword(s):  
Amino Acids ◽  
Creatine Kinase ◽  
Brain Cortex ◽  
Kinase Activity ◽  
Kinetic Studies ◽  
Creatine Kinase Activity ◽  
Branched Chain ◽  
The Brain

Kinetic studies on the inhibition of creatine kinase activity by 3-butyl-1-phenyl-2-(phenyltelluro)oct-en-1-one in the cerebral cortex of rats

2012 ◽  
Vol 50 (10) ◽  
pp. 3468-3474 ◽  
Author(s):  
Rodrigo Binkowski de Andrade ◽  
Tanise Gemelli ◽  
Robson Brum Guerra ◽  
Cláudia Funchal ◽  
Clovis Milton Duval Wannmacher
Keyword(s):  
Creatine Kinase ◽  
Cerebral Cortex ◽  
Kinase Activity ◽  
Kinetic Studies ◽  
Creatine Kinase Activity
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