scholarly journals Role of the amino-terminal region of streptokinase in the generation of a fully functional plasminogen activator complex probed with synthetic peptides

1998 ◽  
Vol 7 (3) ◽  
pp. 637-648 ◽  
Author(s):  
Deepak Nihalani ◽  
Rajesh Kumarm ◽  
K. Rajagopal ◽  
Girish Sahni
Keyword(s):  
Plasminogen Activator ◽  
Synthetic Peptides ◽  
Terminal Region ◽  
Amino Terminal ◽  
Activator Complex

scholarly journals Role of the amino-terminal region of the DnaA protein in opening of the duplex DNA at theoriCregion

1999 ◽  
Vol 176 (1) ◽  
pp. 163-167 ◽  
Author(s):  
Shinji Mima ◽  
Yoshihiro Yamagachi ◽  
Taemi Kondo ◽  
Tomofusa Tsuchiya ◽  
Tohru Mizushima
Keyword(s):  
Terminal Region ◽  
Duplex Dna ◽  
Dnaa Protein ◽  
Amino Terminal

scholarly journals Role of positive charge on the amino-terminal region of the signal peptide in protein secretion across the membrane.

1982 ◽  
Vol 79 (11) ◽  
pp. 3438-3441 ◽  
Author(s):  
S. Inouye ◽  
X. Soberon ◽  
T. Franceschini ◽  
K. Nakamura ◽  
K. Itakura ◽  
...  
Keyword(s):  
Protein Secretion ◽  
Signal Peptide ◽  
Positive Charge ◽  
Terminal Region ◽  
Amino Terminal

The Role of the Unique Motifs in the Amino-Terminal Region of PKN on Its Enzymatic Activity

1996 ◽  
Vol 220 (3) ◽  
pp. 963-968 ◽  
Author(s):  
Michinori Kitagawa ◽  
Hideki Shibata ◽  
Masanao Toshimori ◽  
Hideyuki Mukai ◽  
Yoshitaka Ono
Keyword(s):  
Enzymatic Activity ◽  
Terminal Region ◽  
Amino Terminal

SYNTHETIC PEPTIDE PROBES OF FACTOR VIII IMMUNOLOGY AND FUNCTION

1987 ◽  
Author(s):  
C A Fulcher ◽  
R A Houghten ◽  
S de Graaf Mahoney ◽  
J R Roberts ◽  
T S Zimmerman
Keyword(s):  
Amino Acid ◽  
Synthetic Peptide ◽  
Synthetic Peptides ◽  
Polyclonal Antibodies ◽  
Keyhole Limpet Hemocyanin ◽  
Significant Inhibition ◽  
Terminal Region ◽  
Amino Terminal ◽  
Microtiter Plates ◽  
Peptide Immunogen

In order to develop specific immunologic reagents for mapping functionally important sites on FVIII, we have prepared rabbit polyclonal antibodies against synthetic peptides of FVIII derived from regions along the entire FVIII amino acid sequence. To date, a total of 70 peptides have been synthesized and characterized by amino acid and HPLC analysis. The peptides were coupled to keyhole limpet hemocyanin with glutaraldehyde as a linkage reagent and used to immunize rabbits. Antisera were tested by ELISA assay on polystyrene microtiter plates coated with either the peptide immunogen, or purified FVIII. The antisera were also tested for their ability to inhibit FVIII clotting activity and to react with separated FVIII polypeptides on immunoblots.Of the 70 peptides, all reacted with the peptide immunogen, 45 reacted with purified FVIII and 33 reacted with FVIII on immunoblots. Because we had obtained evidence that cleavage of the amino terminal region of the 80 kDa polypeptide may play a role in FVIII activation by thrombin, a series of partially overlapping peptides, 15 residues in length, were synthesized in this area. After affinity purifying these antibodies on columns of FVIII immobilized on agarose, adjusting the antibodies to equal antigen binding titers by dot immunoblotting and testing for inhibition of FVIII activity, only one antibody could strongly inhibit FVIII clotting activity. This inhibition could be blocked by the peptide itself at nanomolar concentrations and no significant inhibition could be shown by antibodies to partially overlapping peptides individually, or in combination. These data suggest that a site important to FVIII function can be localized to a 15 amino acid residue region of the 80 kDa polypeptide of FVIII. In addition, a second inhibitoryantibody was identified which was produced against a peptide in the carboxy terminal region of the 54 kDa thrombin fragment of FVIII and this area is currently being studied in a similar manner. In addition, two monoclonal anti-FVIII synthetic peptide antibodies have been produced which react with purified FVIII on immunoblots. One of these antibodies also functions as an immunoadsorbent when linked to agarose and FVII can be purified in this manner, using the synthetic peptide as eluant. It is evident that antibodies to synthetic peptides of FVIII can be useful probes of FVIII structure, function and interactions as well as being of use in FVIII purification.

Human Parathyroid Hormone: Immunological Properties of the Amino Terminus and of Synthetic Fragments

1974 ◽  
Vol 47 (6) ◽  
pp. 567-576 ◽  
Author(s):  
G. N. Hendy ◽  
P. M. Barling ◽  
J. L. H. O'Riordan
Keyword(s):  
Parathyroid Hormone ◽  
Synthetic Peptides ◽  
Terminal Region ◽  
Amino Terminus ◽  
Human Parathyroid Hormone ◽  
Immunological Properties ◽  
Immunological Assay ◽  
Amino Terminal

1. Immunological assay systems specific to the amino-terminal region of parathyroid hormone were used to study the properties of human parathyroid hormone (HPTH 1-84) and of synthetic peptides. One of these peptides (HPTH 1-34A) had the sequence proposed by Niall, Sauer, Jacobs, Keutmann, Segre, O'Riordan, Aurbach & Potts (1974). The others (HPTH 1-34B and C, synthesized by different methods) had the sequence proposed by Brewer, Fairwell, Ronan, Sizemore & Arnaud (1972). 2. HPTH 1-84 and HPTH 1-34A behaved identically in a number of these systems. In some systems, especially those using antisera obtained by immunizing with HPTH 1-34A, differences were, however, found. These may be due to conformational or other physical differences or to the presence of an immunologically incomplete site in the fragment. 3. In systems in which HPTH 1–84 and HPTH 1-34A behaved identically the peptides with the sequence proposed by Brewer et al. (1972) were of low reactivity by 100–2000-fold.

scholarly journals Structural and functional role of the amino-terminal region of porcine cytosolic aspartate aminotransferase

1991 ◽  
Vol 266 (7) ◽  
pp. 4187-4193
Author(s):  
Y Fukumoto ◽  
S Tanase ◽  
F Nagashima ◽  
S Ueda ◽  
K Ikegami ◽  
...  
Keyword(s):  
Aspartate Aminotransferase ◽  
Functional Role ◽  
Terminal Region ◽  
Amino Terminal
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