scholarly journals In vitro methionine oxidation of escherichia coli -derived human stem cell factor: Effects on the molecular structure, biological activity, and dimerization

1996 ◽  
Vol 5 (6) ◽  
pp. 1165-1173 ◽  
Author(s):  
Yueh-Rong Hsu ◽  
Linda O. Narhi ◽  
Christopher Spahr ◽  
Keith E. Langley ◽  
Hsieng S. Lu
Keyword(s):  
Molecular Structure ◽  
Escherichia Coli ◽  
Biological Activity ◽  
Stem Cell ◽  
Stem Cell Factor ◽  
Methionine Oxidation ◽  
Human Stem Cell ◽  
Human Stem Cell Factor

Effects of recombinant human stem cell factor (SCF) on the growth of human progenitor cells in vitro

1991 ◽  
Vol 148 (3) ◽  
pp. 503-509 ◽  
Author(s):  
Giovanni Migliaccio ◽  
Anna Rita Migliaccio ◽  
Maurice L. Druzin ◽  
Patricia-Jane V. Giardina ◽  
Krisztina M. Zsebo ◽  
...  
Keyword(s):  
Stem Cell ◽  
Progenitor Cells ◽  
Stem Cell Factor ◽  
Human Stem Cell ◽  
Human Stem Cell Factor

scholarly journals Recombinant human stem cell factor, a c-kit ligand, stimulates hematopoiesis in primates

Blood ◽  
1991 ◽  
Vol 78 (8) ◽  
pp. 1975-1980 ◽  
Author(s):  
RG Andrews ◽  
GH Knitter ◽  
SH Bartelmez ◽  
KE Langley ◽  
D Farrar ◽  
...  
Keyword(s):  
Stem Cell ◽  
Stem Cell Factor ◽  
Absolute Number ◽  
Human Stem Cell ◽  
Clinical Role ◽  
Gm Csf ◽  
Macrophage Colony ◽  
Human Stem Cell Factor

Abstract Recombinant human stem cell factor (SCF) is homologous with recombinant rat SCF (rrSCF) and is a ligand for c-kit. We determined the influence of SCF on hematopoiesis in vitro and in vivo in baboons. In vitro, SCF alone stimulated little growth of hematopoietic colony-forming cells from baboon marrow, but did increase the number of colonies formed in response to erythropoietin (Epo), interleukin-3 (IL-3), and granulocyte- macrophage colony-stimulating factor (GM-CSF). In vivo, SCF caused an increase in the peripheral blood of the number of erythrocytes, neutrophils, lymphocytes, monocytes, eosinophils, and basophils. In marrow, it caused an increase in marrow cellularity and in the absolute number of colony-forming unit-granulocyte-monocyte (CFU-GM) and burst- forming unit-erythroid (BFU-E) in marrow following infusion of SCF. The in vivo stimulation of multiple lymphohematopoietic lineages corroborates previous in vitro studies and suggests a potentially important clinical role for SCF.

Human stem cell factor does not affect the morphology and expression of functionally relevant molecules of Langerhans cells in vitro

1998 ◽  
Vol 16 ◽  
pp. S96
Author(s):  
Francesca Prignano ◽  
Gianni Gerlini ◽  
Nicola Pimpinelli ◽  
Paolo Romagnoli ◽  
Benvenuto Giannotti
Keyword(s):  
Stem Cell ◽  
Stem Cell Factor ◽  
Langerhans Cells ◽  
Human Stem Cell ◽  
Human Stem Cell Factor

High-level expression of human stem cell factor fused with erythropoietin mimetic peptide in Escherichia coli

2006 ◽  
Vol 47 (2) ◽  
pp. 477-482 ◽  
Author(s):  
Lin Su ◽  
Song-Sen Chen ◽  
Ke-Gong Yang ◽  
Chang-Zheng Liu ◽  
Yan-Li Zhang ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Stem Cell ◽  
Stem Cell Factor ◽  
Human Stem Cell ◽  
High Level Expression ◽  
Mimetic Peptide ◽  
High Level ◽  
Human Stem Cell Factor

scholarly journals Recombinant human stem cell factor, a c-kit ligand, stimulates hematopoiesis in primates

Blood ◽  
1991 ◽  
Vol 78 (8) ◽  
pp. 1975-1980 ◽  
Author(s):  
RG Andrews ◽  
GH Knitter ◽  
SH Bartelmez ◽  
KE Langley ◽  
D Farrar ◽  
...  
Keyword(s):  
Stem Cell ◽  
Stem Cell Factor ◽  
Absolute Number ◽  
Human Stem Cell ◽  
Clinical Role ◽  
Gm Csf ◽  
Macrophage Colony ◽  
Human Stem Cell Factor

Recombinant human stem cell factor (SCF) is homologous with recombinant rat SCF (rrSCF) and is a ligand for c-kit. We determined the influence of SCF on hematopoiesis in vitro and in vivo in baboons. In vitro, SCF alone stimulated little growth of hematopoietic colony-forming cells from baboon marrow, but did increase the number of colonies formed in response to erythropoietin (Epo), interleukin-3 (IL-3), and granulocyte- macrophage colony-stimulating factor (GM-CSF). In vivo, SCF caused an increase in the peripheral blood of the number of erythrocytes, neutrophils, lymphocytes, monocytes, eosinophils, and basophils. In marrow, it caused an increase in marrow cellularity and in the absolute number of colony-forming unit-granulocyte-monocyte (CFU-GM) and burst- forming unit-erythroid (BFU-E) in marrow following infusion of SCF. The in vivo stimulation of multiple lymphohematopoietic lineages corroborates previous in vitro studies and suggests a potentially important clinical role for SCF.

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