scholarly journals Characterization of a quaternary-structured folding intermediate of an antibody Fab-fragment

2008 ◽  
Vol 4 (5) ◽  
pp. 917-924 ◽  
Author(s):  
Hauke Lilie ◽  
Rainer Jaenicke ◽  
Johannes Buchner
Keyword(s):  
Folding Intermediate ◽  
Fab Fragment

Characterization of a folding intermediate of apoplastocyanin trapped by proline isomerization

Biochemistry ◽  
1993 ◽  
Vol 32 (46) ◽  
pp. 12299-12310 ◽  
Author(s):  
Shohei Koide ◽  
H. Jane Dyson ◽  
Peter E. Wright
Keyword(s):  
Folding Intermediate ◽  
Proline Isomerization

scholarly journals Characterization of Humanized Antibodies Secreted by Aspergillus niger

2004 ◽  
Vol 70 (5) ◽  
pp. 2567-2576 ◽  
Author(s):  
Michael Ward ◽  
Cherry Lin ◽  
Doreen C. Victoria ◽  
Bryan P. Fox ◽  
Judith A. Fox ◽  
...  
Keyword(s):  
Aspergillus Niger ◽  
Mutant Form ◽  
Fab Fragment ◽  
Antibody Purification ◽  
Heavy Chains ◽  
Charge Induction ◽  
Mammalian Cell Cultures ◽  
Significant Difference ◽  
Hydrophobic Charge Induction Chromatography

ABSTRACT Two different humanized immunoglobulin G1(κ) antibodies and an Fab′ fragment were produced by Aspergillus niger. The antibodies were secreted into the culture supernatant. Both light and heavy chains were initially synthesized as fusion proteins with native glucoamylase. After antibody assembly, cleavage by A. niger KexB protease allowed the release of free antibody. Purification by hydrophobic charge induction chromatography proved effective at removing any antibody to which glucoamylase remained attached. Glycosylation at N297 in the Fc region of the heavy chain was observed, but this site was unoccupied on approximately 50% of the heavy chains. The glycan was of the high-mannose type, with some galactose present, and the size ranged from Hex6GlcNAc2 to Hex15GlcNAc2. An aglycosyl mutant form of antibody was also produced. No significant difference between the glycosylated antibody produced by Aspergillus and that produced by mammalian cell cultures was observed in tests for affinity, avidity, pharmacokinetics, or antibody-dependent cellular cytotoxicity function.

Engineering and Characterization of a Chimeric Anti-platelet Glycoprotein Ibα Monoclonal Antibody and Preparation of Its Fab Fragment

Hybridoma ◽  
2010 ◽  
Vol 29 (2) ◽  
pp. 125-132 ◽  
Author(s):  
Jianfeng Yang ◽  
Shundong Ji ◽  
Ningzheng Dong ◽  
Yiming Zhao ◽  
Changgeng Ruan
Keyword(s):  
Monoclonal Antibody ◽  
Platelet Glycoprotein ◽  
Fab Fragment

scholarly journals Phage Display Cloning and Characterization of Monoclonal Antibody Genes and Recombinant Fab Fragment against the CD98 Oncoprotein

2001 ◽  
Vol 92 (12) ◽  
pp. 1313-1321 ◽  
Author(s):  
Kunihiko Itoh ◽  
Kazuyuki Inoue ◽  
Kazumi Hirooka ◽  
Kumiko Maruyama ◽  
Mayumi Ohkawa ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Phage Display ◽  
Fab Fragment

scholarly journals Characterization of a Shiga Toxin 1-Neutralizing Recombinant Fab Fragment Isolated by Phage Display System

2004 ◽  
Vol 203 (4) ◽  
pp. 295-303 ◽  
Author(s):  
Kazuyuki Inoue ◽  
Kunihiko Itoh ◽  
Hiroshi Nakao ◽  
Tae Takeda ◽  
Toshio Suzuki
Keyword(s):  
Phage Display ◽  
Shiga Toxin ◽  
Display System ◽  
Fab Fragment

α-Lytic Protease Precursor:  Characterization of a Structured Folding Intermediate†

Biochemistry ◽  
1999 ◽  
Vol 38 (15) ◽  
pp. 4728-4735 ◽  
Author(s):  
D. Eric Anderson ◽  
Reuben J. Peters ◽  
Barry Wilk ◽  
David A. Agard
Keyword(s):  
Folding Intermediate ◽  
Protease Precursor
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